Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Antioxidant and Hepatoprotective Activity of Root extract of Baliospermum montanum (Willd) Muell Arg


Affiliations
1 Institute of Pharmacy, Guru Ghasidas University, Bilaspur (C.G.), India
2 School of Pharmacy, Chouksey Engineering College, Bilaspur (CG), India
     

   Subscribe/Renew Journal


The hydroalcoholic extract of Baliospermum montanum Muell Arg ischolar_main was screened for their possible hepatoprotective and antioxidant effect. Carbon tetrachloride (CCl4) and ethanol were used to produce liver toxicity. The hydroalcoholic extract of plant ischolar_main was administered and biochemical parameters were estimated. Concurrently, silymarin was used as a standard hepatoprotective agent. The ischolar_main extract was also studied for in vitro total antioxidant capacity by ABTS assay. The results indicated that biochemical parameter changes produced by CCL4 and ethanol were restored to normal by the hydroalcoholic extract. The hydroalcoholic extract showed significant (p<0.05) protection of liver cell against CCL4 and ethanol-induced hepatocellular injury in a dose-dependent manner. The effects of hydroalcoholic extract were comparable with silymarin. Hepatoprotection produced by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation. The antioxidant potential of the extract may be attributed to the presence of phenolic and flavonoid compounds. The ischolar_main extract of Baliospermum montanum Muell Arg. exhibited significant antioxidant and hepatoprotective activity.

Keywords

Antioxidants Activity, Ethanol, Hepatoprotective Activity, Baliospermum montanum Muell Arg.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Mayuresh R, Andrze P, Patrycja LK, Wojeich Z, Rafal, F. Herbal medicine for treatment and prevention of liver diseases. Journal Preclinical and Clinical Research. 2014; 2: 55-60.

  • Feng Y, Wang N, Ye X, Li H, Feng Y, et al. Hepatoprotective effect and its possible mechanism of Coptidis rhizoma aqueous extract on carbon tetrachloride-induced chronic liver hepatotoxicity in rats. J. Ethnopharmacol. 2011; 138: 683–690.

  • Ansari JA. Ansari Therapeutic Approaches in Management of Drug-induced Hepatotoxicity. J Biol. Sci. 2010; 10:386–395.

  • Dianzani MU, Muzia G, Biocca ME, Canuto RA. Lipid peroxidation in fatty liver induced by caffeine in rats. International Journal of Tissue Reaction. 1991; 13: 79-85.

  • Tiwari P, Ahirwar D, Chandy A, Ahirwar B. Evaluation of hepaptoprotective activity of alcoholic and aqueous extract of Selaginell alepidophylla. Asian Pac. J. Trop Dis. 2014; 4: 81-86.

  • Ronis MJ, Hakkar R, Korourian S, Albano E. 2004. Alcohalic liver disease in rat fed ethanol as part of oral and intragastric low- carbohydrate liquid diets. Exp. Biol. Med. 2004; 229: 351-60.

  • Chattopadhyay RR. Possible mechanisms of hepatoprotective activity of Azadirachta indica leaf extract. J. Ethnopharmacol. 2003; 89: 217-219.

  • Ravindra GM, Raju RW. 2008. Baliospermum montanum (Danti): Ethnobotany, Phytochemistry and Pharmacology- A review. Int. J. Green Pharmacy. 2008; 2:194-199.

  • Nadkarni KM. The Indian Materia Medica. 3rd ed. Popular Prakashan, Bombay; 1998.

  • Chopra RN, Chopra IC, Handi KL, Kopor LD. Indigenous drugs of India, Academic Publishers, Calcutta; 1994.

  • Goel AK, Sahoo AK, Mudgal VA. Contribution to the Ethnobotany of Santal Pargana, Bihar. Bull. Bot Survey Ind. 1984; 31: 22-26.

  • Bhatt AV, Nair KV, Nair CA, Puri HS. Ethnobotany studies in the silent valley and adjoining areas. Bull. Med. Ethnobot. Res. 1982; 3: 153-161.

  • Shanmugasundaram P, Venkatraman S. Hepatoprotective and antioxidant effects of Hygrophilla auriculata (K. Schun) Heine Acantheceae ischolar_main extract. J. Ethnopharmacol. 2006; 104: 124-128.

  • Zafer SR, Mujahid A. Anti-hepatotoxicity effect of ischolar_main and ischolar_main culture of Cichoriumin btybus Linn. J. Ethnopharmacol. 1998; 63: 227-231.

  • Allam RM, Selim DA, Ghoneim AI, Radwan MM, Nofal SM. Et al. Hepatoprotective effects of Astragalus kahiricus ischolar_main extract against ethanol-induced liver apoptosis in rats. Chin. J. Nat. Med. 2013; 11: 354-361.

  • Afeefa CM, Surendra M, Rasheed A, Rajesh V, Babu G. et al. Comprehensive review on in-vitro and in-vitro models for hepatoprotective activity. J. Pharm. Sci. Res. 2016; 8: 184-189.

  • Husain A, Ahmes M, Kajeeb SA, Khan AG, Alghamdi FA. Quantitative analysis of total phenolics, flavonoids contents and HPTLC fingerprinting for standardization of Glycyrrhiza glabra L. ischolar_mains. Herbal Medicine. 2015; 1: 1-9.

  • Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocher P. Vidal Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry. 2006; 97: 654-660.

  • Gupta A, Sheth NR, Pandey S, Yadav JS, Joshi SV. 2015. Screening of flavonoids rich fractions of the three Indian medicinal plants used for the management of liver diseases. Revista Brasiteria de Farmacognosia. 2015; 25: 485-490.

  • Pal P, Ray S. 2016. Alcoholic liver diseases: A comprehensive review. European Medical Journal. 2016; 85-92.

  • Kamble PB, Kulkarni DK. 2010. Medicinal plants traditionally used in jaundice from Bhor region, Pune district. Geobios. 2010; 37, 9-12.

  • Ighodaro O, Ebuehi O. Aqueous leaf extracts of Ocimum gratissimum potentiates activities of plasma and hepatic antioxidant enzymes in rats. Niger. Q. J. Hosp. Med. 2008; 19: 106–109.

  • Patil B. In vitro study of cytoprotection by Aloe vera leaf gel against APAP (AAP) mediated oxidative stress in rat liver slices. Int. J. Adva. Biotech. Res. 2010; 2: 436-441.

  • Baranisrinivasan P, Elumalai EK, Sivakumar C, Viviyan ST, David E. Hepatoprotective effect of Enicostemma littorale blume and Eclipta albaduring ethanol induced oxidative stress in albino rats. Int. J. Pharmacol. 2009; 5: 268–272.

  • Janbaz K, Saeed S, Gilani A. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine. 2004; 11: 424–430.

  • John S, Kale M, Rathore N, Bhatnagar D. Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes. J. Nutr. Biochem. 2001; 12: 500–504.

  • Mak DH, Ip SP, Li PC, Poon MK, Ko KM. 1996. Alterations in tissue glutathione antioxidant system in Streptozotocin-induced diabetic rats. Mol. Cell Biochem. 1996; 162: 153–158.

  • Sen S, Chakroborty R, Sridhar C, Reddy YSR, Biplab D. Free radicals antioxidants disease and phytommedicine current status and future prospects. International Journal of Pharmaceutical Science Review and Research. 2010; 39: 91-100.

  • Cichoz-Lach H, Michalak A. 2014. Oxidative stress as a crucial factor in liver diseases. World J. Gastroenterol. 2014; 20: 8082–8091.

  • Singal AK, Jampana SC, Weinman SA. Antioxidants as therapeutic agents for liver disease. Liver Int. 2011; 31: 1432–1448.

  • Duryee MJ, Klaseen LW, Thiele GM. 2007. Immunological response in alcoholic liver disease. World J Gastroenterol. 2007; 13: 4938–4946.

  • Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB. Resources and biological activities of natural polyphenols. Nutrients. 2014; 6: 6020–6047.

  • Mahmud ZA, Bachar SC, Qais N. Antioxidant and hepatoprotective activities of ethanolic extracts of leaves of Premna esculenta Roxb. against carbon tetrachloride-induced liver damage in rats. J. Young Pharm. 2012; 4: 228–234.

  • Yen GC, Duh PD, Tsai CL. Relationship between antioxidant activity and maturity of peanut hulls. J. Agric. Food Chem. 1993; 41: 67–70.

  • Chang JC, Lin CC, Wu SJ, Lin DL, Wang SS, Miaw CL. Antioxidative and hepatoprotective effects of Physalis peruviana extract against acetaminophen-induced liver injury in rats. Pharm. Biol. 2008; 46: 724–31.

  • Yoshikawa M, Ninomiya K, Shimoda H, Nishida N, Matsuda H. Hepatoprotective and antioxidative properties of Salacia reticulata: Preventive effects of phenolic constituents on CCl4-induced liver injury in mice. Biol. Pharm. Bull. 2002; 25: 72–6.

  • Zhod Z, Sun X, Kang Y. Methionine protection against alcohol liver injury through inhibition of oxidative stress. Exp. Biol. Med. 2002; 222: 214-222.

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice CE. 1999. Antioxidant activity applying improved ABTS radical cation decolorization assay Free Radicals. Biol. Med. 1999; 26: 1231-1237.

  • Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y. 2015. The role of oxidative stress and antioxidants in liver diseases. Int. J. Mol. Sci. 2015; 16: 26087-26124.


Abstract Views: 191

PDF Views: 0




  • Antioxidant and Hepatoprotective Activity of Root extract of Baliospermum montanum (Willd) Muell Arg

Abstract Views: 191  |  PDF Views: 0

Authors

B. Ahirwar
Institute of Pharmacy, Guru Ghasidas University, Bilaspur (C.G.), India
D. Ahirwar
School of Pharmacy, Chouksey Engineering College, Bilaspur (CG), India

Abstract


The hydroalcoholic extract of Baliospermum montanum Muell Arg ischolar_main was screened for their possible hepatoprotective and antioxidant effect. Carbon tetrachloride (CCl4) and ethanol were used to produce liver toxicity. The hydroalcoholic extract of plant ischolar_main was administered and biochemical parameters were estimated. Concurrently, silymarin was used as a standard hepatoprotective agent. The ischolar_main extract was also studied for in vitro total antioxidant capacity by ABTS assay. The results indicated that biochemical parameter changes produced by CCL4 and ethanol were restored to normal by the hydroalcoholic extract. The hydroalcoholic extract showed significant (p<0.05) protection of liver cell against CCL4 and ethanol-induced hepatocellular injury in a dose-dependent manner. The effects of hydroalcoholic extract were comparable with silymarin. Hepatoprotection produced by decreasing the activity of serum enzymes, bilirubin, and lipid peroxidation. The antioxidant potential of the extract may be attributed to the presence of phenolic and flavonoid compounds. The ischolar_main extract of Baliospermum montanum Muell Arg. exhibited significant antioxidant and hepatoprotective activity.

Keywords


Antioxidants Activity, Ethanol, Hepatoprotective Activity, Baliospermum montanum Muell Arg.

References