Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In-Vivo Antioxidant Activity of Hibiscus plantifolius Stems


Affiliations
1 Department of Pharmaceutical Chemistry, Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India
2 Department of Pharmaceutical and Phytochemistry, Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh,-522503, India
3 Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India
     

   Subscribe/Renew Journal


Objective: Hibiscus plantifolius is a shrub with various medicinal uses and belongs to the Malvaceae family. The present study is aimed at determining in vivo antioxidant activity of MEHP using the paracetamol-induced hepatotoxicity test in rats. Methods: The animals were randomly divided into 7 experimental groups and administered with test solutions. Rats were divided into seven groups and received 10% DMSO (negative control), Paracetamol (2 gm/kg bd. wt.), Silymarin (10 mg/kg bd.wt.) or 50mg/kg MEHP (50, 150 and 300 mg/kg) orally once daily for 7 consecutive days. The oral administration of paracetamol was performed 3 hours after the last extract administration on the 7th day. The blood and liver tissues were collected and subjected to biochemical and microscopical analysis. Results: In this study, it was observed that MEHP extract significantly (p<0.05) increased hepatic SOD and CAT activities in paracetamol-induced liver damage in rats. The antioxidant activity was exhibited due to presence of flavonoids and tannins, phenolic compounds which was present methanolic extract of Hibiscus plantifolius. Conclusion: The antioxidant activity was exhibited due to presence of flavonoids and tannins, phenolic compounds which was present methanolic extract of Hibiscus plantifolius.

Keywords

In-Vivo Antioxidant Activity, Hepato Protective, DPPH,, Nitric Oxide, Hibiscus plantifolius.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Ellman GL., et al 1959, Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77.
  • Sinha AK., et al 1972, Colorimetric assay of catalase. Analyt Biochem 47:389–94.
  • Marklund S., et al 1974, Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474.
  • Lin CC., et al 1997, Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J Ethnopharmacol 56:193–200.
  • Aldridge WN., et al 1981, Mechanism of toxicity: New concepts are required in toxicology. Trends in Pharmacol Sci 2 :228–231
  • Gilani AH., et al 2005, Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the Carum copticum seed extract. J Ethnopharmacol 98:127–135.
  • Curtis SJ., et al 1972, Serum enzyme derived from liver cell fractions .The response to carbon tetrachloride intoxication in rats. Gastroentrology 62(1): 84-92.
  • Prakash J., et al 2001, Chemopreventive activity of Withania somnifera in experimentally induced fibrosarcoma tumours in Swiss albino mice. Phytother Res 15:240–244.

Abstract Views: 310

PDF Views: 0




  • In-Vivo Antioxidant Activity of Hibiscus plantifolius Stems

Abstract Views: 310  |  PDF Views: 0

Authors

K. Sowjanya
Department of Pharmaceutical Chemistry, Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India
R. Ratna Manjula
Department of Pharmaceutical and Phytochemistry, Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh,-522503, India
V. Vaishnavi
Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India
B. Keerthi Sai
Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India
K. Divya
Nirmala College of Pharmacy, Atmakur (V), Guntur, Andhra Pradesh-522503, India

Abstract


Objective: Hibiscus plantifolius is a shrub with various medicinal uses and belongs to the Malvaceae family. The present study is aimed at determining in vivo antioxidant activity of MEHP using the paracetamol-induced hepatotoxicity test in rats. Methods: The animals were randomly divided into 7 experimental groups and administered with test solutions. Rats were divided into seven groups and received 10% DMSO (negative control), Paracetamol (2 gm/kg bd. wt.), Silymarin (10 mg/kg bd.wt.) or 50mg/kg MEHP (50, 150 and 300 mg/kg) orally once daily for 7 consecutive days. The oral administration of paracetamol was performed 3 hours after the last extract administration on the 7th day. The blood and liver tissues were collected and subjected to biochemical and microscopical analysis. Results: In this study, it was observed that MEHP extract significantly (p<0.05) increased hepatic SOD and CAT activities in paracetamol-induced liver damage in rats. The antioxidant activity was exhibited due to presence of flavonoids and tannins, phenolic compounds which was present methanolic extract of Hibiscus plantifolius. Conclusion: The antioxidant activity was exhibited due to presence of flavonoids and tannins, phenolic compounds which was present methanolic extract of Hibiscus plantifolius.

Keywords


In-Vivo Antioxidant Activity, Hepato Protective, DPPH,, Nitric Oxide, Hibiscus plantifolius.

References