Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Determination of Amoxicillin Trihydrate by Analytical Spectrophotometry


Affiliations
1 Department of Chemistry, Faculty of Science, University of Aleppo, Syrian Arab Republic
     

   Subscribe/Renew Journal


Simple and rapid spectrophotometric method has been developed for the determination of Amoxicillin trihydrate in bulk and capsules formulation. The method is based on the formation of yellow ion-pair complex between Amoxicillin trihydrate and Bromocresol purple in Chloroform medium. The absorption maximum of the complex was found to be 408 nm. Different parameters affecting the reaction such as: effect of solvents, time, reagent concentration, correlation ratio, etc. were optimized. The formed complex was quantified spectrophotometrically at absorption maximum. Linearity range was 2.097 – 20.97 μg/mL, regression analysis showed a good correlation coefficient R2 = 0.9998. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.25 μg/mL and 0.76 μg/mL. The average percent recovery was found to be (98.66 - 100.00) % for Amoxicillin trihydrate. This study was applied on two Syrian pharmaceutical trademarks: (Amoxicillin APHAMEA 500, Amoxicillin THAMECO 500). The method was successfully applied for the determination of Amoxicillin trihydrate in pharmaceutical capsules formulation. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.


Keywords

Amoxicillin Trihydrate, Bromocresol Purple, Spectrophotometric Method.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Dhiraj SN, Chandrakant G, Bonde1 SJ, Surana G, Venkateshwarlu PG. Development and validation of RP-HPLC method for estimation of amoxicillin trihydrate and flucloxacillin sodium in capsule dosage form. Journal of Applied Chemical Researches.2009; 1(3): 935-939.

  • Mrudul RK, Ravin MJ. A new spectrophotometric method for determination of amoxicillin using bromocresol green. World Journal of Pharmacy and Pharmaceutical sciences. 2014; 3(2): 1340-1348.

  • Petkova V, Tsvetkova D D, Obreshkova D P, Dimitrov M, Papanov S, Atanaso P. Validation of TLC – densitometric method for quality control of amoxicillin in dosage drug products. World Journal of Pharmacy and Pharmaceutical Sciences. 2014; 3(5): 995-1008.

  • Beg S, Hasnain M S, Swain S, Kohli K. Validation stability- indicating LC method for estimation of amoxicillin trihydrate in pharmaceutical dosage forms and time –dependent Release formulations. International Journal of Pharmaceutical Sciences and Nanotechnology. 2011; 4(2): 1423-1427.

  • Wenhong L, Catharina Y W A. Determination of amoxicillin residues in animal tissues by solid-phase extraction and liquid chromatography with fluorescence detection. Journal of AOAC International. 2000; 83 (1): 20-25.

  • Batrawi N, Wahdan Sh, Al-Rimawi F. A validated stability-indicating HPLC method for simultaneous determination of amoxicillin and enrofloxacin combination in an injectable suspension. Sci. Pharm. 2017; 85(6):10. 3390.

  • Abreu L R Pires, Ortiz R A M, Castro S C D, Pedrazzoli J J. HPLC determination of amoxicillin comparative bioavailability in healthy volunteers after a single dose administration. J Pharm Pharmaceut Sci. 2003; 6(2):223-230.

  • Salam A H, Al-Ameri, Najlah MH, Al-Waeli. Differential pulse polarographic study of amoxicillin and ciprofloxacin and its determination in pharmaceuticals. International Journal of Bio Analytical Methods and Bio Equivalence Studies (IJBMBS). 2016; 3(1): 47-54.

  • Fouladgar M, Hadjmohammad M R, Khalilzadeh M A, Biparva P, Teymoori N, Beitollah H. Voltammetric determination of amoxicillin at the electrochemical Sensor ferrocenedicarboxylic acid multi wall carbon nanotubes paste electrode. International Journal of Electrochemical Science. 2011; (6): 1355 – 1366.

  • Prakash K, Narayana R P, Shanta K K, Lakshmi N. Spectrophotometric estimation of amoxicillin trihydrate in bulk and pharmaceutical dosage form. E-Journal of Chemistry. 2008; 5(S2): 1114-1116.

  • Ahmed M, Babu S G, Shetty S K A. Development and validation of amoxicillin by RP-HPLC method in bulk drug and pharmaceutical dosage forms. International Journal of Chem Tech Research. 2011; 3(3): 1037-104.

  • Hassouna M, Mohamed M. Development and validation of RP-HPLC method for determination of amoxicillin residues and application to NICOMAC coating machine. Journal of Analytical and Pharmaceutical Research. 2018; 7(5):586-594.

  • Rajan V R, Rajesh N M. Simultaneous determination of amoxicillin trihydrate and bromhexine hydrochloride in pharmaceutical dosage by reverse phase high performance liquid chromatography. Der Pharma Chemica. 2013; 5(1):273-278.

  • Uddina M N, Dasb S, Khana Sh H, Shilla S K, Bhuiyanb H R, Karim R. Simultaneous determination of amoxicillin and chloramphenicol and their drug interaction study by the validated UPLC method. Journal of Taibah University for Science. 2016; (10): 755–765.

  • Pharmacopeia USP34 NF 29 2011.


Abstract Views: 190

PDF Views: 0




  • Determination of Amoxicillin Trihydrate by Analytical Spectrophotometry

Abstract Views: 190  |  PDF Views: 0

Authors

Saad Antakli
Department of Chemistry, Faculty of Science, University of Aleppo, Syrian Arab Republic
Leon Nejem
Department of Chemistry, Faculty of Science, University of Aleppo, Syrian Arab Republic
Walaa Alsheikh Ahmad
Department of Chemistry, Faculty of Science, University of Aleppo, Syrian Arab Republic

Abstract


Simple and rapid spectrophotometric method has been developed for the determination of Amoxicillin trihydrate in bulk and capsules formulation. The method is based on the formation of yellow ion-pair complex between Amoxicillin trihydrate and Bromocresol purple in Chloroform medium. The absorption maximum of the complex was found to be 408 nm. Different parameters affecting the reaction such as: effect of solvents, time, reagent concentration, correlation ratio, etc. were optimized. The formed complex was quantified spectrophotometrically at absorption maximum. Linearity range was 2.097 – 20.97 μg/mL, regression analysis showed a good correlation coefficient R2 = 0.9998. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.25 μg/mL and 0.76 μg/mL. The average percent recovery was found to be (98.66 - 100.00) % for Amoxicillin trihydrate. This study was applied on two Syrian pharmaceutical trademarks: (Amoxicillin APHAMEA 500, Amoxicillin THAMECO 500). The method was successfully applied for the determination of Amoxicillin trihydrate in pharmaceutical capsules formulation. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.


Keywords


Amoxicillin Trihydrate, Bromocresol Purple, Spectrophotometric Method.

References