Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Antioxidant and Anticancer potential of Methanolic extract of Kigelia pinnata fruits against DENA induced Hepatocellular Carcinoma in rats


Affiliations
1 Krupanidhi College of Pharmacy, Carmelaram Post, Varthur Hobli, Bengaluru-560035, India
     

   Subscribe/Renew Journal


The aim of the present study is to study the preventive nature of Kigelia pinnata methanolic extract (MEKP)during DiEthyl Nitros Amine (DENA) induced hepatocellular carcinoma in rats. Phytochemical screening for the methanolic extract of Kigelia pinnata was carried out. The methanolic extract of the fruits was tested for antioxidant property by in vitro DPPH radical scavenging assay and reducing power assay and also anticancer activity of methanolic extract of Kigelia pinnata was evaluated in DENA induced Sprague Dawley rats at the doses of 250 and 500 mg/kg body weight orally and both doses were administered for 14 weeks. After 24 h of the last dose, the rats were sacrificed and antitumor effect of KMPE extract was assessed by evaluating biochemical parameters such as AFP, AST, ALT, ALP and LDH to evaluate anticancer activity of the extract. MDA and SOD levels in the liver tissue of each group were estimated for determining in vivo antioxidant activity of the plant. Histopathological studies were carried out. MEKP showed the presence of alkaloids, glycosides, flavonoids, tannins, saponins, reducing sugars and phenolic compounds. MEKP showed concentration dependent free radical scavenging activity and increase in reducing power activity. Comparative analysis on the KPME treatment groups revealed that high dose (500mg/kg body weight showed significant reduction in the serum level of enzymes. Also, there was dose dependent effect of MEKP in the MDA and SOD level in the liver tissue. Histopathological studies showed the decrease in the damages to the liver tissue in the MEKP treatment groups. Hence the methanolic extract of Kigelia pinnata possesses anti-hepatocellular activity which may be due to its hepatoprotective action. This may be due to the presence of flavonoids. Further studies can be carried out to elucidate the exact mechanism of its activity as an antioxidant and anticancer agent. The methanolic extract of Kigelia pinnata possesses anti-hepatocellular activity which may be due to its hepatoprotective action. This may be due to the presence of flavonoids. Further studies can be carried out to elucidate the exact mechanism of its activity as an antioxidant and anticancer agent.

Keywords

Kigelia pinnata, DENA, Hepatocellular Carcinoma, Antioxidant, Phenobarbital.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Julius A, Ramachandran Vedasendiyar, Archana Devakannan, Sujatha Rajaraman, Balamurugan Rangasamy, Saravanan V. Effect of Hesperidin for its Anti-Proliferative Activity on Liver Cancer and Cardio Vascular Diseases. Research J. Pharm. and Tech. 2017; 10(1): 307-8

  • Singh R, Sharma J and Goyal PK. Prophylactic role of Averrhoa carambola (star fruit) extract against chemically induced hepatocellular carcinoma in Swiss albino mice. Advances in pharmacological sciences. 2014.

  • Mohammed FZ, Sultan AS and Abas AM. Chemopreventive and therapeutic effect of capsaicin against diethylnitrosamine induced liver injury and hepatocellular carcinoma in rats. Int. J. Biol. Pharm. Res. 2014;5(8):630-42.

  • Yoshiji H, Nakae D, Kinugasa T, Matsuzaki M, Denda A, Tsujii T, Konishi Y. Inhibitory effect of dietary iron deficiency on the induction of putative preneoplastic foci in rat liver initiated with diethylnitrosamine and promoted by phenobarbital. British journal of cancer. 1991 Nov;64(5):839-45.

  • Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. African Journal of Microbiology Research. 2009 Dec 31;3(13):981-96.

  • Dhungana BR, Jyothi Y, Das K. Kigelia Pinnata: Exploration of Potential Medicinal Usage in Human Ailments. Journal of Pharmaceutical Research. 2016 Dec 1;15(4):138-46.

  • Ren W, Qiao Z, Wang H, Zhu L, Zhang L. Flavonoids: promising anticancer agents. Medicinal research reviews. 2003 Jul;23(4):519-34.

  • Procházková D, Boušová I and Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011 Jun 1;82(4):513-23.

  • Man S, Gao W, Zhang Y, Huang L, Liu C. Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia. 2010 Oct 1;81(7):703-14.

  • Gayathri Gunalan, M. Suresh Kumar, N. Sangeetha. Preliminary Phytochemical Analysis and In Vitro Oxidant Scavenging Activity of Rosemary officinalis. Research J. Pharm. and Tech. 4(10): Oct. 2011;1588-90.

  • Jiby Elias, Rajesh MG, Anish NP, Deepa P, Jayan N. In Vitro Antioxidant Activity of the Methanolic Extract of Simaruba glauca DC. Asian J. Research Chem. 2010 Apri 3(2):312-15.

  • Byahatti VV, Khan AM, Patil SH, Choudhury A. Preliminary Phytochemical Investigation and In-vitro Antioxidant Activity of Bergenia ciliata Leaves. Research J. Pharm. and Tech. 2010 June 3(2):;399-401.

  • Sreepriya M, Bali G. Chemopreventive effects of embelin and curcumin against N-nitrosodiethylamine/phenobarbital-induced hepatocarcinogenesis in Wistar rats. Fitoterapia. 2005 Sep 1;76(6):549-55.

  • Fiume L, Bolondi L, Busi C, Chieco P, Kratz F, Lanza M, Mattioli A, Di Stefano G. Doxorubicin coupled to lactosaminated albumin inhibits the growth of hepatocellular carcinomas induced in rats by diethylnitrosamine. Journal of hepatology. 2005 Oct 1;43(4):645-52.

  • Rajasekaran S. Anti-ulcer activity of different extract of Kigelia pinnata in experimental animals. Asian J Pharm Clin Res. 2014;7:80-3.

  • Chandra YP, Viswanathswamy AH. Chemo Preventive Effect of Rutin Against N-Nitrosodiethylamine-Induced and Phenobarbital-Promoted Hepatocellular Carcinoma in Wistar Rats. Indian journal of pharmaceutical education and research. 2018 Jan 1;52(1):78-86.

  • Patil Suhas A., Joshi V.G., Sambrekar S.N. , Desai N. S. Hepatoprotective and Antioxidant Potential of Extracts from Whole Plant of Solanum surattense Burm. Research J. Pharmacognosy and Phytochemistry 2011; 3(1): 17-21.

  • Devasagayam TP, Tarachand U. Decreased lipid peroxidation in the rat kidney during gestation. Biochemical and biophysical research communications. 1987 May 29;145(1):134-8.

  • Sujit Dash, Amaresh Chandra Sahoo, Bishwanath Mishra. In vitro and In vivo Antioxidant assessment and Hepatoprotective activity of Aponogeton natans (Linn.) Engl. and Krause on Diclofenac sodium induced Liver Toxicity. Research J. Pharm. and Tech 2018;11(10):4431-38.

  • Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. june 2010; 3(2): 551-55.

  • Gauri Karwani, Siddhraj S. Sisodia. Hepatoprotective activity of Chenopodium album Linn. in Ethanol induced Hepatotoxicity in Rats. Research J. Pharm. and Tech. 2015 june 8(6):669-73

  • Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life sciences. 2004 Mar 12;74(17):2157-84.

  • Suresh Kumar Sutrakar, Drutpal Singh Baghel. Biochemical Parameters Variations in Type–II Diabetes Mellitus: Special Reference in Rewa Region. Asian J. Research Chem. 7(10): October- 2014; Page 877-81.

  • Kobayashi T, Kawakubo T. Prospective investigation of tumor markers and risk assessment in early cancer screening. Cancer. 1994 Apr 1;73(7):1946-53.

  • Rocchi E, Seium Y, Camellini L, Casalgrandi G, Borghi A, D'Alimonte P, Cioni G. Hepatic tocopherol content in primary hepatocellular carcinoma and liver metastases. Hepatology. 1997 Jul;26(1):67-72.

  • Banakar MC, Paramasivan SK, Chattopadhyay MB, Datta S, Chakraborty P, Chatterjee M, Kannan K, Thygarajan E. 1α, 25-dihydroxyvitamin D3 prevents DNA damage and restores antioxidant enzymes in rat hepatocarcinogenesis induced by diethylnitrosamine and promoted by phenobarbital. World journal of gastroenterology. 2004 May 1;10(9):1268-73.

  • Zwart LL, Meerman JH, Commandeur JN, Vermeulen NP. Biomarkers of free radical damage: applications in experimental animals and in humans. Free Radical Biology and Medicine. 1999 Jan 1;26(1-2):202-26.

  • Sallie R, Michael Tredger J, Williams R. Drugs and the liver part 1: Testing liver function. Biopharmaceutics & drug disposition. 1991 May;12(4):251-9.

  • Julius A, Ramachandran Vedasendiyar, Archana Devakannan, Sujatha Rajaraman, Balamurugan Rangasamy, Saravanan V. Effect of Hesperidin for its Anti-Proliferative Activity on Liver Cancer and Cardio Vascular Diseases. Research J. Pharm. and Tech. 2017; 10(1): 307-308.


Abstract Views: 264

PDF Views: 0




  • Antioxidant and Anticancer potential of Methanolic extract of Kigelia pinnata fruits against DENA induced Hepatocellular Carcinoma in rats

Abstract Views: 264  |  PDF Views: 0

Authors

Bishwo Raj Dhungana
Krupanidhi College of Pharmacy, Carmelaram Post, Varthur Hobli, Bengaluru-560035, India
Y. Jyothi
Krupanidhi College of Pharmacy, Carmelaram Post, Varthur Hobli, Bengaluru-560035, India
Das Kuntal
Krupanidhi College of Pharmacy, Carmelaram Post, Varthur Hobli, Bengaluru-560035, India
P. Radhika
Krupanidhi College of Pharmacy, Carmelaram Post, Varthur Hobli, Bengaluru-560035, India

Abstract


The aim of the present study is to study the preventive nature of Kigelia pinnata methanolic extract (MEKP)during DiEthyl Nitros Amine (DENA) induced hepatocellular carcinoma in rats. Phytochemical screening for the methanolic extract of Kigelia pinnata was carried out. The methanolic extract of the fruits was tested for antioxidant property by in vitro DPPH radical scavenging assay and reducing power assay and also anticancer activity of methanolic extract of Kigelia pinnata was evaluated in DENA induced Sprague Dawley rats at the doses of 250 and 500 mg/kg body weight orally and both doses were administered for 14 weeks. After 24 h of the last dose, the rats were sacrificed and antitumor effect of KMPE extract was assessed by evaluating biochemical parameters such as AFP, AST, ALT, ALP and LDH to evaluate anticancer activity of the extract. MDA and SOD levels in the liver tissue of each group were estimated for determining in vivo antioxidant activity of the plant. Histopathological studies were carried out. MEKP showed the presence of alkaloids, glycosides, flavonoids, tannins, saponins, reducing sugars and phenolic compounds. MEKP showed concentration dependent free radical scavenging activity and increase in reducing power activity. Comparative analysis on the KPME treatment groups revealed that high dose (500mg/kg body weight showed significant reduction in the serum level of enzymes. Also, there was dose dependent effect of MEKP in the MDA and SOD level in the liver tissue. Histopathological studies showed the decrease in the damages to the liver tissue in the MEKP treatment groups. Hence the methanolic extract of Kigelia pinnata possesses anti-hepatocellular activity which may be due to its hepatoprotective action. This may be due to the presence of flavonoids. Further studies can be carried out to elucidate the exact mechanism of its activity as an antioxidant and anticancer agent. The methanolic extract of Kigelia pinnata possesses anti-hepatocellular activity which may be due to its hepatoprotective action. This may be due to the presence of flavonoids. Further studies can be carried out to elucidate the exact mechanism of its activity as an antioxidant and anticancer agent.

Keywords


Kigelia pinnata, DENA, Hepatocellular Carcinoma, Antioxidant, Phenobarbital.

References