Research Journal of Pharmacy and Technology

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Preparation of Silver Nanoparticles using Nutmeg Oleoresin and its Antimicrobial Activity against Oral Pathogens


Affiliations
1 Saveetha Dental College,Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India
2 Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India
     

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Introduction: Green synthesis of silver nanoparticles has been increasingly preferred nowadays over physical and chemical approaches. In this study, silver nanoparticles are synthesised using nutmeg oleoresin at room temperature. Materials and methods: Nutmeg oleoresin mediated silver nanoparticles was prepared using 1 milli molar of silver nitrate. The prepared nanoparticles were confirmed using UV-vis spectrophotometer. Different concentrates of the synthesised nutmeg oleoresin mediated silver nanoparticles was tested against Streptococcus mutans and Enterococcus faecalis using agar well diffusion method and MIC will be determined. Results: It has been observed that the anti bacterial effect of the prepared AgNP’s was almost similar against all the organisms used in the study with a maximum ZOI against Pseudomonas sp. and minimum ZOI against Enterococcus faecalis. It has also been observed that the antibacterial activity of the AgNP’s increased with increasing concentration. Conclusion: The synthesised nutmeg oleoresin mediated AgNP’s showed tremendous antimicrobial activity against the oral pathogens: Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Pseudomonas sp. with increased activity against Pseudomonas sp. Thus novel biological approach for the synthesis of AgNP’s using nutmeg oleoresin is a eco-friendly method and can be used in the development new drugs against oral pathogens.


Keywords

Nutmeg Oleoresin, Silver Nanoparticles, Oral Pathogens.
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  • K Paulkumar, G Gnanajobitha, M Vanaja, Shunmugam Rajeshkumar, C Malarkodi and G Annadurai Piper nigrum leaf and stem assisted green synthesis of silver nanoparticles and evaluation of its antibacterial activity against plant pathogens, (2014) The Scientific World Journal http://dx.doi.org/10.1155/2014/829894 Impact Factor 1.2

  • Bose S, Shinde H, Karikalan K, Lalitha P, Mandal AK. Antibacterial, antiinflamatory, and antiproliferative activity of silver nanoparticles synthesized from leaf extract of Azadirachtaindica A. juss. Research Journal of Pharmacy and Technology. 2016 Dec 1;9(12):2422.

  • M. Vanaja, K. Paulkumar, S. Rajeshkumar, G. GnanaJobitha, C. Malarkodi M. Sivakavinesan, and G. Annadurai Degradation of methylene blue using biologically synthesized silver nanoparticles (2014) Bioinorganic Chemistry and Applications http://dx.doi.org/10.1155/2014/742346. Impact Factor 2.081.

  • M. Vanaja, K. Paulkumar, S. Rajeshkumar, G. GnanaJobitha, C. Malarkodi M. Sivakavinesan, and G. Annadurai Herbal plant synthesis of antibacterial silver nanoparticles by Solanum trilobatum and its characterization (2014) International Journal of Metals http://dx.doi.org/10.1155/2014/692461.

  • S Rajeshkumar (2016) Synthesis of silver nanoparticles using Fresh bark of Pongamiapinnata and its antibacterial action against gram positive and gram negative pathogen Resource-Efficient Technologies 2 (2016) 30–35.

  • U. Kanagavalli, A. Mohamed Sadiq, Sathishkumar, S. Rajeshkumar. Plant Assisted Synthesis of Silver Nanoparticles Using Boerhaavia diffusa Leaves Extract and Evolution of Antibacterial Activity. Research J. Pharm. and Tech 2016;9(8):1064-1068.

  • Khodke PB, Popat RR, Burakale PV, Chinchole PP, Shrikhande VN. Silver nanoparticles-A review. Research Journal of Pharmacy and Technology. 2017 Jun 1;10(6):1820.

  • S. Asha, A. Asha, S. Rajeshkumar Evaluation of Phytochemical constituents and Antimicrobial Activity of silver nanoparticle synthesized Ipomoea nil against selected Pathogens Asian Journal of Pharmaceutical and Clinical Research Asian J Pharm Clin Res, Vol 10, Issue 3, 2017, 1-5. DOI: http://dx.doi.org/10.22159/ajpcr.2017.v10i3.15986

  • S. Asha, P. Thirunavukkarasu, S. Rajeshkumar Green synthesis of silver nanoparticles using mirabilisjalapa aqueous extract and their antibacterial activity against respective microorganisms (2017) Research Journal of Pharmacy Technology 2017 10(3) : 811-817. DOI:10.5958/0974-360X.2017.00153.6

  • S Venkat Kumar, Karpagambigai S, Jacquline Rosy P and S Rajeshkumar Phyto-assisted Synthesis of Silver Nanoparticles using Solanum nigrum and Antibacterial Activity against Salmonella typhi and Staphylococcus aureus Mechanics, Materials Science & Engineering (2017) Vol9. doi:10.2412/mmse.86.22.967.

  • Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular aspects of Medicine. 2006 Feb 1;27(1):1-93.

  • Rajeshkumar S and Bharath L V Mechanism of plant-mediated synthesis of silver nanoparticles - A review on biomolecules involved, characterisation and antibacterial activity Chemico-Biological Interactions 273 (2017) 219-227. http://dx.doi.org/10.1016/j.cbi.2017.06.019.

  • Jana U, Pal S, Mohanta GP, Manna PK, Manavalan R. Nanoparticles: A Potential Approach for Drug Delivery. Research Journal of Pharmacy and Technology. 2011;4(7):1016-9.

  • S Rajeshkumar (2016) Green synthesis of different sized antimicrobial silver nanoparticles using different parts of plants – A Review International Journal of ChemTech Research 9 (4) 197-208

  • Prasad BN, Padmesh TV. Common duckweed (Lemna minor) assisted green synthesis of silver nanoparticles as potent anti-fungal nanomaterial. Research Journal of Pharmacy and Technology. 2014 Sep 1;7(9):3.

  • Asha S, Thirunavukkarasu P, Rajeshkumar S. Green Synthesis of Silver Nanoparticles using Mirabilisjalapa Aqueous Extract and their Antibacterial Activity against Respective Microorganisms. Research Journal of Pharmacy and Technology. 2017 Mar 1;10(3):811.

  • Vilas V, Philip D, Mathew J. Essential oil mediated synthesis of silver nanocrystals for environmental, anti-microbial and antioxidant applications. Materials Science and Engineering: C. 2016 Apr 1;61:429-36.

  • Eby DM, Schaeublin NM, Farrington KE, Hussain SM, Johnson GR. Lysozyme catalyzes the formation of antimicrobial silver nanoparticles. ACS nano. 2009 Apr 3;3(4):984-94.

  • Hendler N, Fadeev L, Mentovich ED, Belgorodsky B, Gozin M, Richter S. Bio-inspired synthesis of chiral silver nanoparticles in mucin glycoprotein—the natural choice. Chemical Communications. 2011;47(26):7419-21.

  • Gautam S, Dubey P, Gupta MN. A facile and green ultrasonic-assisted synthesis of BSA conjugated silver nanoparticles. Colloids and Surfaces B: Biointerfaces. 2013 Feb 1;102:879-83.

  • Periasamy G, Karim A, Gibrelibanos M, Gebremedhin G. Nutmeg (Myristica fragrans Houtt.) oils. InEssential Oils in Food Preservation, Flavor and Safety 2016 (pp. 607-616).

  • Kapoor IP, Singh B, Singh G, De Heluani CS, De Lampasona MP, Catalan CA. Chemical composition and antioxidant activity of essential oil and oleoresins of nutmeg (Myristica fragrans Houtt.) fruits. International journal of food properties. 2013 Jul 4;16(5):1059-70.

  • Gupta AD, Bansal VK, Babu V, Maithil N. Chemistry, antioxidant and antimicrobial potential of nutmeg (Myristica fragrans Houtt). Journal of Genetic engineering and Biotechnology. 2013 Jun 1;11(1):25-31.

  • Moyler DA. Oleoresins, tinctures and extracts. InFood Flavorings 1991 (pp. 54-86). Springer, Boston, MA.

  • Rodianawati I, Hastuti P, Cahyanto MN. Nutmeg's (Myristica fragrans Houtt) oleoresin: effect of heating to chemical compositions and antifungal properties. Procedia Food Science. 2015 Jan 1;3:244-54.

  • Morsy NF. A comparative study of nutmeg (Myristica fragrans Houtt.) oleoresins obtained by conventional and green extraction techniques. Journal of food science and technology. 2016 Oct 1;53(10):3770-7.

  • Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir. 1996 Feb 7;12(3):788-800.

  • Vilchis-Nestor AR, Sánchez-Mendieta V, Camacho-López MA, Gómez-Espinosa RM, Camacho-López MA, Arenas-Alatorre JA. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Materials Letters. 2008 Jun 30;62(17-18):3103-5.

  • Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M. Synthesis of gold nanotriangles and silver nanoparticles using Aloevera plant extract. Biotechnology progress. 2006;22(2):577-83

  • Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of colloid and interface science. 2004 Jul 1;275(1):177-82.

  • Yu-sen EL, Vidic RD, Stout JE, McCartney CA, Victor LY. Inactivation of Mycobacterium avium by copper and silver ions. Water Research. 1998 Jul 1;32(7):1997-2000.

  • Rajeshkumar S, Kannan. C and Annadurai. G (2012) Synthesis and characterization of antimicrobial silver nanoparticles using marine brown seaweed Padinatetrastromatica Drug Invention Today4 (10), 511-513.

  • Lansdown AB. Silver in health care: antimicrobial effects and safety in use. In Biofunctional textiles and the skin 2006 (Vol. 33, pp. 17-34). Karger Publishers.

  • Oppermann RV, Johansen JR. Effect of fluoride and non‐fluoride salts of copper, silver and tin on the acidogenicity of dental plaque in vivo. European Journal of Oral Sciences. 1980 Dec;88(6):476-80.


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  • Preparation of Silver Nanoparticles using Nutmeg Oleoresin and its Antimicrobial Activity against Oral Pathogens

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Authors

T. Pranati
Saveetha Dental College,Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India
Roy Anitha
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India
S. Rajeshkumar
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India
Thangavelu Lakshmi
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India

Abstract


Introduction: Green synthesis of silver nanoparticles has been increasingly preferred nowadays over physical and chemical approaches. In this study, silver nanoparticles are synthesised using nutmeg oleoresin at room temperature. Materials and methods: Nutmeg oleoresin mediated silver nanoparticles was prepared using 1 milli molar of silver nitrate. The prepared nanoparticles were confirmed using UV-vis spectrophotometer. Different concentrates of the synthesised nutmeg oleoresin mediated silver nanoparticles was tested against Streptococcus mutans and Enterococcus faecalis using agar well diffusion method and MIC will be determined. Results: It has been observed that the anti bacterial effect of the prepared AgNP’s was almost similar against all the organisms used in the study with a maximum ZOI against Pseudomonas sp. and minimum ZOI against Enterococcus faecalis. It has also been observed that the antibacterial activity of the AgNP’s increased with increasing concentration. Conclusion: The synthesised nutmeg oleoresin mediated AgNP’s showed tremendous antimicrobial activity against the oral pathogens: Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Pseudomonas sp. with increased activity against Pseudomonas sp. Thus novel biological approach for the synthesis of AgNP’s using nutmeg oleoresin is a eco-friendly method and can be used in the development new drugs against oral pathogens.


Keywords


Nutmeg Oleoresin, Silver Nanoparticles, Oral Pathogens.

References