Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract


Affiliations
1 Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Porur, Chennai–600116., India
2 Dept. of Pharmacology, GSL Medical College, Rajamundhary, A.P., India
     

   Subscribe/Renew Journal


Objective Vitex negundo L. (verbenaceae), is a large aromatic shrub with various medicinal properties. This current work has been proposed to explore antioxidant property of methanolic extract of Vitex negundo by various in vitro assays. Methods. The in vitro antioxidant action has been determined via DPPH assay, Nitric oxide scavenging activity, and by assessing reducing power. Results Methanolic extract of Vitex negundo showed a very good anti-radical activity in scavenging DPPH radical with maximum % inhibition of 81.87±1.53% at 2000μg/0.1 ml concentration and nitric oxide radical with maximum % inhibition of 75.29±1.33% at 2000μg/ml. Reducing power of Vitexnegundois83.75±0.57% at 2000μg/0.05 ml concentration. Adding on, methanolic extract of Vitex negundo also showed significant anti-inflammatory activity with maximum % inhibition of 79.81 at 500ug. Conclusion Taken together the result shows that methanolic extract of Vitex negundo possesses substantial free radical scavenging action and anti-inflammatory properties. Hence more research is warranted for ascertaining the effective compound contributing towards its antioxidant and anti-inflammatory activities.

Keywords

Vitex negundo, Methanolic Leaf Extract, Antioxidant Potential, DPPH Scavenging Activity, NO Scavenging Activity, Total Antioxidant Activity And Reducing Power, Anti-Inflammatory.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Lemire J, Alhasawi A, Appanna VP, Tharmalingam S and Appanna VD . Metabolic defence against oxidative stress: the road less travelled so far. Journal of applied microbiology 2017;123: 798-809.

  • Lobo V, Patil A, Phatak A and Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews 2010;4: 118-126.

  • Phaniendra A, Jestadi DB and Periyasamy L. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry 2015;30: 11-26.

  • Figueroa la, Navarro LB, Vera MP, Petricevich VL. Antioxidant activity, total phenolic and flavonoid contents, and Cytotoxicity evaluation of bougainvillea xbuttiana. International journal of pharmacy and pharmaceutical sciences 2014; 6: 497-502

  • Xu D-P, Li Y, Meng X, Zhou T, Zhou Y, Zheng J et al . Natural Antioxidants in Foods and Medicinal Plants: Extraction, Assessment and Resources. International Journal of Molecular Sciences 2017;18: 96.

  • Meziti A, Bouriche H, Hichem M, Kada S, Senator A, Dimertas I . Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus And Zizyphus Vulgaris Methanol Extracts. International Journal of Pharmacy and Pharmaceutical Sciences 2017; 9: 69-76

  • Hassan W, Noreen H, Rehman S, Gul S, Kamal MA, Kamdem JP et al . Oxidative Stress and Antioxidant Potential of One Hundred Medicinal Plants. Current topics in medicinal chemistry 2017;17: 1336-1370.

  • Venkateswarlu K. Vitex negundo: Medicinal Values, Biological Activities, Toxicity Studies, Phytopharmacological Actions. . International Journal of Pharmaceutical and Phytopharmacological Research 2012;2: 126-133.

  • Ullah Z, Ullah R, Shah A, Ahmad I and Haider S. Phytochemical and Biological evaluation of Vitex negundo Linn: A Review. International Journal of Pharmaceutical Sciences and Research 2012;3: 2421-2431.

  • Bano U, Jabeen A, Ahmed A and M. AS. Therapeutic uses of Vitex nigundo. World Journal of Pharmaceutical Research 2015;4: 589-606.

  • Ahuja S, Ahuja S and Ahuja U. Nirgundi (Vitex negundo)–Nature’s Gift to Mankind. Asian Agri-History 2015;19: 5-32.

  • Chan E, Wong S and Tuck Chan H. Casticin from Vitex species: a short review on its anticancer and anti-inflammatory properties. J Integr Med 2018a;16: 147-152.

  • Braca A, Tommasi N, Di Bari L, Pizza C, Politi M and Morelli I. Antioxidant principles from Bauhinia terapotensis. Journal of Natural Products 2001;64: 892-895.

  • Sreejayan and Rao MN. Nitric oxide scavenging by curcuminoids. The Journal of pharmacy and pharmacology 1997;49: 105-7.

  • Mizushima Y and Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. The Journal of pharmacy and pharmacology 1968;20: 169-73.

  • Sakat S, Juvekar AR and Gambhire MN. In vitro antioxidant and anti- inflammatory activity of methanol extracts of Oxalis Corniculata Linn. Int J Pharm Sci 2010;20: 146-56.

  • Jayaprakasha GK, Singh RP and Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chemistry 2001;73: 285-290.

  • Chawla AS, Sharma AK, Handa SS and Dhar KL. Chemical investigation and anti-inflammatory activity of Vitex negundo seeds. J Nat Prod 1992;55: 163-7.

  • Khan A, Naz S, Farooq U, Shahid M, Ullah I, Ali I et al. Bioactive chromone constituents from Vitex negundo alleviate pain and inflammation. Journal of pain research 2018;11: 95-102.

  • Li YT, Pang DR, Zhu ZX, Huo HX, Ren Y, Peng ZT, Zheng J et al. Chemical constituents from the fruits of Vitex negundo var. cannabifolia and their biological activities in vitro. China journal of Chinese materia medica 2016b;41: 4197-4203.

  • Saklani S, Mishra AP, Chandra H, Atanassova MS, Stankovic M, Sati B et al. Comparative Evaluation of Polyphenol Contents and Antioxidant Activities between Ethanol Extracts of Vitex negundo and Vitex trifolia L. Leaves by Different Methods. Plants 2017;6.

  • Tiwari OP and Tripathi YB. Antioxidant properties of different fractions of Vitex negundo Linn. Food Chemistry 2007;100: 1170-1176.

  • Tondon V and Gupta RK. Effect of Vitex negundo on oxidative stress. Indian Journal of Pharmacology 2005;37: 37-45.

  • Zheng G, Lue Z and Chen D. Zheng G. Studies on the antioxygenic composition of Vitex plant leaves. Guangdong Gongye. Daxue Xuebao 1999; 41-47: 2.

  • Kadir FA, Kassim NM, Abdulla MA and Yehye WA. PASS-predicted Vitex negundo activity: antioxidant and antiproliferative properties on human hepatoma cells-an in vitro study. BMC Complementary and Alternative Medicine 2013; 13: 343.

  • Ono M, Nishida Y, Masuoka C, Li JC, Okawa M, Ikeda et al. Lignan derivatives and a norditerpene from the seeds of Vitex negundo. J Nat Prod 2004;67: 2073-5.

  • Chan EWC, Wong SK and Chan HT. Casticin from Vitex species: a short review on its anticancer and anti-inflammatory properties. J Integr Med 2018b;16: 147-152.

  • Jivrajani M, Ravat N, Anandjiwala S and Nivsarkar M . Antiestrogenic and Anti-Inflammatory Potential of n-Hexane Fraction of Vitex negundo Linn Leaf Extract: A Probable Mechanism for Blastocyst Implantation Failure in Mus musculus. International scholarly research notices 2014; 241946.

  • Li MM, Su XQ, Sun J, Gu YF, Huang Z, Zeng KW et al. Anti-inflammatory ursane-and oleanane-type triterpenoids from Vitex negundo var. cannabifolia. J Nat Prod 2014;77: 2248-54.

  • Zheng CJ, Zhao XX, Ai HW, Lin B, Han T, Jiang YP et al. Therapeutic effects of standardized Vitex negundo seeds extract on complete Freund's adjuvant induced arthritis in rats. Phytomedicine: international journal of phytotherapy and phytopharmacology 2014;21: 838-46.

  • Chattopadhyay P, Hazarika S, Dhiman S, Upadhyay A, Pandey A, Karmakar S et al. Vitex negundo inhibits cyclooxygenase-2 inflammatory cytokine-mediated inflammation on carrageenan-induced rat hind paw edema. Pharmacognosy research 2012;4: 134-7.

  • Siddiqui S, Saify ZS, Jamali KS, Tufail P, Kanwal A, Kamal A et al. Neuroprotective capabilities of Vitex negundo in primary hippocampal neurons. Pakistan journal of pharmaceutical sciences 2018;31: 341-344.


Abstract Views: 369

PDF Views: 0




  • In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract

Abstract Views: 369  |  PDF Views: 0

Authors

D. Anusha
Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Porur, Chennai–600116., India
Nagaraju Kancherla
Dept. of Pharmacology, GSL Medical College, Rajamundhary, A.P., India
Darling Chellathai
Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, SRIHER, Porur, Chennai–600116., India

Abstract


Objective Vitex negundo L. (verbenaceae), is a large aromatic shrub with various medicinal properties. This current work has been proposed to explore antioxidant property of methanolic extract of Vitex negundo by various in vitro assays. Methods. The in vitro antioxidant action has been determined via DPPH assay, Nitric oxide scavenging activity, and by assessing reducing power. Results Methanolic extract of Vitex negundo showed a very good anti-radical activity in scavenging DPPH radical with maximum % inhibition of 81.87±1.53% at 2000μg/0.1 ml concentration and nitric oxide radical with maximum % inhibition of 75.29±1.33% at 2000μg/ml. Reducing power of Vitexnegundois83.75±0.57% at 2000μg/0.05 ml concentration. Adding on, methanolic extract of Vitex negundo also showed significant anti-inflammatory activity with maximum % inhibition of 79.81 at 500ug. Conclusion Taken together the result shows that methanolic extract of Vitex negundo possesses substantial free radical scavenging action and anti-inflammatory properties. Hence more research is warranted for ascertaining the effective compound contributing towards its antioxidant and anti-inflammatory activities.

Keywords


Vitex negundo, Methanolic Leaf Extract, Antioxidant Potential, DPPH Scavenging Activity, NO Scavenging Activity, Total Antioxidant Activity And Reducing Power, Anti-Inflammatory.

References