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Selection of the Optimal Conditions for Analysis of Plant Active Pharmaceutical Inulin Ingredients by Thin-layer Chromatography to develop the draft for the National Monograph “Inulin”


Affiliations
1 Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine
2 State Scientific Research Laboratory for Drug Quality Control, National University of Pharmacy, Kharkiv, Ukraine
     

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Inulin is a representative of the class of soluble dietary fibers. One of the stated methods for identification of substances in the monograph “Inulin” of the British Pharmacopoeia 2010 (ВР) is the method of thin-layer chromatography (TLC), in the State Pharmacopoeia of Ukraine (SPhU) the method of TLC for identification of plant active pharmaceutical ingredients (API) of inulin is absent. The Pharmacopoeia provides only quality requirements of such monosaccharides as “Fructose”, “Anhydrous glucose”, “Glucose, monohydrate”, “Anhydrous lactose”, “Lactose monohydrate”. The experimental studies were conducted using nine herbal substances of inulin obtained from plant sources such as chicory, Jerusalem artichoke and agave and two standard substances of inulin with chicory and dahlia. Identification and determination of related impurities of plant API of inulin using TLC were performed according to the method of the monograph “Inulin” of the BP and the method of determination of monosaccharides given in the SPhU. The optimal conditions for the chromatographic determination have been chosen; validation of the method using indicators of specificity, robustness and precision has been conducted. The validated method for analysis of plant API of inulin using TLC has been proposed to be included into the draft for the monograph “Inulin” of the SPhU in the sections “Identification” and “Test for purity”.

Keywords

Inulin, Quality Control, Standardization, Identification, Thin-layer Chromatography.
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  • Roberfroid MB. Inulin - type fructans: functional food ingredients. The Journal of nutrition. 2007, 137 (11): 2493-2502.

  • Barclay T. Inulin - a versatile polysaccharide with multiple pharmaceutical and food chemical uses. Journal of Excipients and Food Chemicals. 2010, 1(3): 27-50.

  • Matvieieva NА. Fructans. Biosynthesis in vivo and in transgenic plants. The Bulletin of Vavilov Society of Geneticists and Breeders of Ukraine. 2010, 8 (2): 312-319.

  • Franck A, De Leenheer L. Inulin. Biopolymers online. Wikey-VCH Verlag GmbH and Co. kGaA, Weinheim, Germany. 2005.

  • Akshay D Patil, Swapnil V Patil, Kishor S Salunkhe, SR. Chaudhari. Review on: Importance and Methods of Reduction of Water Solubility and Swelling of Natural Polysaccharide Polymer for Colon specific Drug Delivery System. Research J. Pharm. and Tech. 6(5). 2013: 447-453.

  • Smielova NM, Yevtifieieva OA. Identification and determination of tests of herbal inulin substances by thin-layer chromatography in accordance with the British Pharmacopoeia. Topical issues of new drugs development: Abstracts of XXIV International Scientific And Practical Conference of Young Scientists And Student (April 20, 2017) in 2 vol. 2017, 1: 202-203.

  • Williams PA, Phillips GO. Handbook of Hydrocolloids. 2010. In: Meyer D, Blaauwhoed JP, ed Inulin. Woodhead Publishing Ltd., Cambridge. 2010: рр. 829-845.

  • Yevtifieieva OA, Smielova NM. The urgency of the development and introduction of the monograph "Inulin" to the State Pharmacopoeia of Ukraine. II international Scientific and Practical Internet Conference «Theoretical and Practical Research of Medicinal Plants». 2016: 106-108.

  • The United States Pharmacopoeia. Rockville: The United State Pharmacopeia, XXХVI ed. 2013.

  • British Pharmacopoeia. Medicinal and pharmaceutical substances. The Stationery Office, London. 2010.

  • State Pharmacopoeia of Ukraine: at 3 volumes. Kharkiv: State enterprise “Ukrainian scientific pharmacopoeia center of medicines quality”, 2-nd ed., Vol. 2. 2014.

  • State Pharmacopoeia of Ukraine: at 3 volumes. 2015. Kharkiv: State enterprise “Ukrainian scientific pharmacopoeia center of medicines quality”, 2-nd ed., Vol. 1. 2015.

  • Jork H, Funk W, Fischer W, Wimmer H. Thin-Layer Chromatography. Reagents and Detection Methods. 1990; 1 a: 464.

  • Georgievskiy VP, Georgievskiy GV, Zinchenko AA. Chromatographic analytical methods provide creation and quality control of medicines in Ukraine. Ukraine, Kharkiv. 2016.

  • Georgievskiy VP. Analytical chemistry in the creation, standardization and quality control of medicines. V. 3. Ukraine, Kharkiv. 2011.

  • Simonovska B. Determination of inulin in foods. Journal of AOAC international. 2000; 83 (3): 675-678.


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  • Selection of the Optimal Conditions for Analysis of Plant Active Pharmaceutical Inulin Ingredients by Thin-layer Chromatography to develop the draft for the National Monograph “Inulin”

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Authors

Nataliia M. Smielova
Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine
Svitlana M. Gubar
State Scientific Research Laboratory for Drug Quality Control, National University of Pharmacy, Kharkiv, Ukraine
Olga A. Yevtifieieva
Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine
Elena M. Bezchasnyuk
State Scientific Research Laboratory for Drug Quality Control, National University of Pharmacy, Kharkiv, Ukraine

Abstract


Inulin is a representative of the class of soluble dietary fibers. One of the stated methods for identification of substances in the monograph “Inulin” of the British Pharmacopoeia 2010 (ВР) is the method of thin-layer chromatography (TLC), in the State Pharmacopoeia of Ukraine (SPhU) the method of TLC for identification of plant active pharmaceutical ingredients (API) of inulin is absent. The Pharmacopoeia provides only quality requirements of such monosaccharides as “Fructose”, “Anhydrous glucose”, “Glucose, monohydrate”, “Anhydrous lactose”, “Lactose monohydrate”. The experimental studies were conducted using nine herbal substances of inulin obtained from plant sources such as chicory, Jerusalem artichoke and agave and two standard substances of inulin with chicory and dahlia. Identification and determination of related impurities of plant API of inulin using TLC were performed according to the method of the monograph “Inulin” of the BP and the method of determination of monosaccharides given in the SPhU. The optimal conditions for the chromatographic determination have been chosen; validation of the method using indicators of specificity, robustness and precision has been conducted. The validated method for analysis of plant API of inulin using TLC has been proposed to be included into the draft for the monograph “Inulin” of the SPhU in the sections “Identification” and “Test for purity”.

Keywords


Inulin, Quality Control, Standardization, Identification, Thin-layer Chromatography.

References