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The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line


Affiliations
1 Universitas Indonesia, Salemba, Jakarta, Indonesia
2 Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, Indonesia
3 Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, Indonesia
4 Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, Indonesia
     

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Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1,327 μg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173,33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.

Keywords

COX-2, Colorectal Cancer, IC50, Immunocytochemistry, MTT Assay, Phaleria macrocarpa.
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  • The Inhibition of Ethanol Extract of Phaleria macrocarpa Stem Bark on COX-2 Expression of HCT116 Colorectal Cancer Cell Line

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Authors

Ratu Shafira Hanifah
Universitas Indonesia, Salemba, Jakarta, Indonesia
Nadia Aida Novitarani
Universitas Indonesia, Salemba, Jakarta, Indonesia
Fadhilah Harmen
Universitas Indonesia, Salemba, Jakarta, Indonesia
Aryo Tedjo
Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, Indonesia
Norma Nur Azizah
Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, Indonesia
Rista Putrianingsih
Department of Medical Chemistry, Universitas Indonesia, Salemba, Jakarta, Indonesia
Wilzar Fachri
Researcher of Cluster Drug Development, Indonesian Medical Education and Research Institute, Universitas Indonesia, Salemba, Jakarta, Indonesia
Kusmardi Kusmardi
Department of Pathology Anatomy, Universitas Indonesia, Salemba, Jakarta, Indonesia

Abstract


Objective of this research to determine the inhibition of ethanol extract of Phaleria macrocarpa Boerl. stem bark on COX-2 expression in colorectal cancer cell line HCT116. Ethanol extract of Phaleria macrocarpa stem bark was prepared for maceration in ethanol. Phaleria macrocarpa stem bark in-vitro anticancer activity was tested with MTT assay on HCT116 colorectal cancer cell line. This study also assessed the effect of ethanol extract of Phaleria macrocarpa stem bark on the inhibition of COX-2 expression in the HCT116 colorectal cancer cell line by counting the H-score from immunocytochemistry staining with help from ImageJ and TMARKER application. Ethanol extract of Phaleria macrocarpa stem bark shows anticancer activity by inhibiting its growth with IC50 of 1,327 μg/ml. One of the pathways is through inhibition of COX-2 expression, shown by the H-score of 173,33 after administration of Phaleria macrocarpa stem bark at a dose of 200 ppm. This study shows that Phaleria macrocarpa stem bark is a colorectal cancer growth inhibitor, one of which is through inhibition of COX-2.

Keywords


COX-2, Colorectal Cancer, IC50, Immunocytochemistry, MTT Assay, Phaleria macrocarpa.

References