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Effect of Copigmentation on Thermal Stability of Hibiscus sabdariffa Anthocyanins


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1 Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India
     

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Synthetic food colors are widely used in foodstuffs to increase consumer acceptance. The use of non permitted colors or overindulgence of permitted colors reported to have toxicity in humans. For substitution, study of natural colorants is becoming an immense area of research. Natural coloring compounds are water soluble anthocyanins, betalains, carminic acid, and the oil soluble carotenoids and chlorophylls. Anthocyanins are blue-purple-red-orange colored plant flavonoids. So far, anthocyanins have not been broadly used in foods and beverages, since they are not as stable as synthetic colors. Copigments are colorless compounds forming intermolecular complex with anthocyanins to present far greater color than natural anthhocyanins thus preventing formation of colorless pseudobase and increases its stability. Purified anthocyanin solutions were copigmented with phenolic acids like gallic acid, tannic acid, ferulic acid and ellagic acids and with flavonoids like rutin and quercetin in different concentrations between anthocyanins and copigments viz. 1:0, 1:2, 1:4, 1:6 at pH values (1.5, 2.5, 3.5, 4.5). Copigmentation was observed using UV–visible spectrophotometry. The maximum effect, revealed by hyperchromic shift was observed at all pH values. Ferulic acid was effectual copigment causing hyperchromic and bathochromic shifts in absorption. Copigmention studies of Hibiscus sabdariffa anthocyanin extract with ferulic acid in 1:6 concentration at acidic pH (pH 2.5) remarkably improved heat stability of Hibiscus sabdariffa anthocyanins.

Keywords

Anthocyanins, Colorant, Copigmentation, Hibiscus,, Ferulic Acid.
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  • Rezaei M. Assessment of Synthetic Dyes in Food Stuffs Produced in Confectioneries and Restaurants in Arak. Iran. J. of Synthetic Thrita. 2015, 4(1): 1-5. http://dx.doi.org/ 10.18869/IAHS.3.2.61

  • Eliana FO, Paulo CS, and Milton CC. Stability of anthocyanin in spinach vine (Basella rubra) fruits. Cien. Inv. Agr. 2007; 34(2): 115-120.

  • Naik P. Food colourants and health issues: are we aware?.Current Science. 2014; 106(2): 25-30.

  • Lauro GJ. A primer on natural colors. Am. Assoc. Cereal Chem 1991; 36(11) : 949-953.

  • Mohamad MF, Nasir SN, Sarmadi MR. Degradation kinetics and color of anthocyanins in aqueous extracts of butterfly pea. Asian Journal of Food and Agro industry. 2011. 4(5):306-315.

  • Parmar M, Gupta U. Biocolors: The New Generation Additives. Int. J. Curr. Microbiol. App. Sci. 2015; 4(7): 688-694.

  • Ghasemifar E, Saeidian S, Setareh P. Stability of seedless red grape anthocyanin under the effect of Chlorogenic acid copigment, heating and UV irradiation. Journal of Novel Applied Sciences. 2013; 2(11):594-597.

  • Boulton R. The Copigmentation of Anthocyanins and Its Role in the Color of Red Wine: A Critical Review. Am. J. Enol. Vitic. 2001; 52 (2) : 67-87.

  • Abyari M, Hedari R, Jamei R. The effect of Heating, UV Irradiation and pH on Stability of Siahe Sardasht Grape Anthocyanin-copigment Complex. Journal of Biological Sciences. 2006; 6(4):638-645.

  • Mahadevan N, Shivali K, Pradeep K. Hibiscus sabdariffa Linn.–An overview. Natural Product Radiance. 2009; 8(1): 77-83.

  • Puro K, Sunjuktar R, Samir S, Ghatak S. Medicinal Uses of Roselle Plant (Hibiscus sabdariffa L.): A Mini Review. Indian Journal of Hill Farming. 2016; 27(1): 81-90.

  • Obouayeba A, Diabate S, Joseph D. Phytochemical and antioxidant activity of Roselle. Research J. of Pharmaceutical, biological and Chemical sciences.2014; 5(2):1453-1465.

  • Harborne, J.B. Phytochemical methods: A Guide to Modern techniques of plants Analysis. Chapman and Hall London., U.K. 1998; pp : 64.

  • Rakkimuthu R, Palmurugan S, Shanmugapriya A. Effect of temperature, light, ph on the stability of anthocyanin pigments in cocculus hirsutus fruits. International Journal of Multidisciplinary Research and Modern Education. 2016; 2(2): 91-96.

  • Rasha K, Mohamed AY, Nagwa MH, Rasmy FM, Abu-Salem. Extraction of Anthocyanin Pigments from Hibiscus sabdariffa L. and Evaluation of their Antioxidant Activity. Middle East Journal of Applied Sciences. 2016; 6(4) : 856-866.

  • Kuntapas K, Singh K, Phetkao S. Effect of pH and anthocyanin concentration on color and antioxidant activity of Clitoria ternatea. J. of Food and Applied Bioscience. 2014; 2(1) :31-46.

  • Munawaroh H, Fadillah G, Saputri, Hanif Q, Hidayat R, Wahyuningsih S. The co-pigmentation of anthocyanin isolated from mangosteen pericarp (Garcinia Mangostana L.) as Natural Dye for Dye Sensitized Solar Cells (DSSC). J. of Materials Science and Engineering. 2016; 107: 1-6.

  • Elham G, Shahriar S, Setareh P. Stability of Seedless Red grape anthocyanin under the effect of Chlorogenic acid copigment, heating and UV irradiation. Journal of Novel Applied Sciences. 2013; 2(11) : 595-597.


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  • Effect of Copigmentation on Thermal Stability of Hibiscus sabdariffa Anthocyanins

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Authors

Reshma V. Jadhav
Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India
Santosh S. Bhujbal
Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, India

Abstract


Synthetic food colors are widely used in foodstuffs to increase consumer acceptance. The use of non permitted colors or overindulgence of permitted colors reported to have toxicity in humans. For substitution, study of natural colorants is becoming an immense area of research. Natural coloring compounds are water soluble anthocyanins, betalains, carminic acid, and the oil soluble carotenoids and chlorophylls. Anthocyanins are blue-purple-red-orange colored plant flavonoids. So far, anthocyanins have not been broadly used in foods and beverages, since they are not as stable as synthetic colors. Copigments are colorless compounds forming intermolecular complex with anthocyanins to present far greater color than natural anthhocyanins thus preventing formation of colorless pseudobase and increases its stability. Purified anthocyanin solutions were copigmented with phenolic acids like gallic acid, tannic acid, ferulic acid and ellagic acids and with flavonoids like rutin and quercetin in different concentrations between anthocyanins and copigments viz. 1:0, 1:2, 1:4, 1:6 at pH values (1.5, 2.5, 3.5, 4.5). Copigmentation was observed using UV–visible spectrophotometry. The maximum effect, revealed by hyperchromic shift was observed at all pH values. Ferulic acid was effectual copigment causing hyperchromic and bathochromic shifts in absorption. Copigmention studies of Hibiscus sabdariffa anthocyanin extract with ferulic acid in 1:6 concentration at acidic pH (pH 2.5) remarkably improved heat stability of Hibiscus sabdariffa anthocyanins.

Keywords


Anthocyanins, Colorant, Copigmentation, Hibiscus,, Ferulic Acid.

References