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Honey a Therapeutic Trial in Osteosarcoma


Affiliations
1 Dept. of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (DU), Sri Ramachandra University, Chennai- 600 116, India
     

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Background: Honey, a multifaceted mixture of enzymes, peptides and amines, has domestic as well as medicinal utility. Recent studies that analyzed its effects in different strains of rat and mouse have pronounced honey as antimetastatic with moderate antitumor effects. In the present study we have tested the cytotoxic activity of diluted unfractionated bee honey against Saos-2 (Sarcoma osteogenic) cell line, a primary osteosarcoma in vitro, to demonstrate that honey can induce apoptosis in primary osteosarcoma cells. Methods: The cytotoxicity effect of honey was evaluated on SAO’S cells and cell cycle analysis was performed using propidium iodide. To look for DNA breaks in cells, DNA of the sample was run through electrophoresis in standard TE buffer to visualize DNA separation pattern on a UV Transilluminator. Results: Cell viability was steadily lost with increasing concentrations of honey and IC50 value for honey was found to be lower than methotrexate drug. Phase contrast microscopy showed that cell appearance was indicative of apoptosis. Results showed that Honey showed an arrest at G0/G1 phase. DNA fragmentation exhibited DNA fragmentation. Methotrexate in combination with honey resulted in the formation of a set of defined wreckage as characteristic of apoptosis. Discussion: The study indicates that honey can be considerably effective against cancer cell lines in vitro. Further in depth study would help to explain the exact mechanism of action of honey against cancer cells and other malignancies.

Keywords

Osteosarcoma, DNA, Honey.
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  • Zakaria NH, Ahmad NZ, Hashim SN, Adnan LH, Halim M, Shariff M, Mohamad N, Mat KC, Bakar NH. Analgesic effect of honey bioactive compounds and its role in reducing morphine tolerance. Journal of Applied Pharmaceutical Science. Vol. 2015 Nov 27; 5(11):146-50.

  • Samarghandian S, Afshari JT, Davoodi S. Honey induces apoptosis in renal cell carcinoma. Pharmacognosy Magazine. 2011 Jan; 7(25):46.

  • Al-Waili NS. Natural honey lowers plasma glucose, C-reactive protein, homocysteine, and blood lipids in healthy, diabetic, and hyperlipidemic subjects: comparison with dextrose and sucrose. Journal of Medicinal Food. 2004 Apr 1; 7(1):100-7.

  • Ferreira IC, Aires E, Barreira JC, Estevinho LM. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chemistry. 2009 Jun 15; 114(4):1438-43.

  • Ahmed S, Othman NH. Honey as a potential natural anticancer agent: a review of its mechanisms. Evidence-Based Complementary and Alternative Medicine. 2013; 2013.

  • Erejuwa O, Sulaiman S, Wahab M. Effects of honey and its mechanisms of action on the development and progression of cancer. Molecules. 2014; 19(2):2497-522.

  • Abubakar MB, Abdullah WZ, Sulaiman SA, Suen AB. A review of molecular mechanisms of the anti-leukemic effects of phenolic compounds in honey. International Journal of Molecular Sciences. 2012 Nov 15; 13(11):15054-73.

  • Fauzi AN, Norazmi MN, Yaacob NS. Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines. Food and Chemical Toxicology. 2011 Apr 1; 49(4):871-8.

  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 2016; 65(1-2):55-63.

  • Del Bino G, Darzynkiewicz Z. Camptothecin, teniposide, or 4′-(9-acridinylamino)-3-methanesulfon-m-anisidide, but not mitoxantrone or doxorubicin, induces degradation of nuclear DNA in the S phase of HL-60 cells. Cancer Research. 1991 Feb 15; 51(4):1165-9.

  • Mohamed M, Sirajudeen KN, Swamy M, Yaacob M, Sulaiman S. Studies on the antioxidant properties of Tualang honey of Malaysia. African Journal of Traditional, Complementary and Alternative Medicines. 2010; 7(1).

  • Chin KY, Mohamea NV, Soelaiman IN, Chin KY, Abdul-Majeed S, Fozi NF, Ima-Nirwana S. The effects of testosterone deprivation induced by buserelin and orchidectomy on bone in a rat model of osteoporosis. Osteoporosis and Sarcopenia. 2017 Aug; 3(3):S37.


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  • Honey a Therapeutic Trial in Osteosarcoma

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Authors

S. Sneha Aishwarya
Dept. of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (DU), Sri Ramachandra University, Chennai- 600 116, India
V. Gowri
Dept. of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (DU), Sri Ramachandra University, Chennai- 600 116, India
D. Darling Chellathai
Dept. of Pharmacology, Sri Ramachandra Institute of Higher Education and Research (DU), Sri Ramachandra University, Chennai- 600 116, India

Abstract


Background: Honey, a multifaceted mixture of enzymes, peptides and amines, has domestic as well as medicinal utility. Recent studies that analyzed its effects in different strains of rat and mouse have pronounced honey as antimetastatic with moderate antitumor effects. In the present study we have tested the cytotoxic activity of diluted unfractionated bee honey against Saos-2 (Sarcoma osteogenic) cell line, a primary osteosarcoma in vitro, to demonstrate that honey can induce apoptosis in primary osteosarcoma cells. Methods: The cytotoxicity effect of honey was evaluated on SAO’S cells and cell cycle analysis was performed using propidium iodide. To look for DNA breaks in cells, DNA of the sample was run through electrophoresis in standard TE buffer to visualize DNA separation pattern on a UV Transilluminator. Results: Cell viability was steadily lost with increasing concentrations of honey and IC50 value for honey was found to be lower than methotrexate drug. Phase contrast microscopy showed that cell appearance was indicative of apoptosis. Results showed that Honey showed an arrest at G0/G1 phase. DNA fragmentation exhibited DNA fragmentation. Methotrexate in combination with honey resulted in the formation of a set of defined wreckage as characteristic of apoptosis. Discussion: The study indicates that honey can be considerably effective against cancer cell lines in vitro. Further in depth study would help to explain the exact mechanism of action of honey against cancer cells and other malignancies.

Keywords


Osteosarcoma, DNA, Honey.

References