Research Journal of Pharmacy and Technology

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Analysis of Antimicrobial Activity of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis Against Multi-Drug Resistant Bacteria


Affiliations
1 University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413, India
2 School of Medicine, Xavier University, Santa Helenastraat 23, Oranjestad, Aruba, Dutch Caribbean, Aruba
     

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Introduction: Emergence of antibiotic resistance bacteria is one of major global problem and researcher are all focused to treat infection caused by antibiotic bacteria. Plants are used for their medicinal property from the ancient time. Plants are rich source of many phytoconstituents that do not possess any side effect. Material and methods: For the present work multidrug resistance bacteria were isolated from hospital soil sample and checked for antibiotic resistance by using antibiotic disk. The microorganisms showing resistant against more than three antibiotics were selected and identified by sequencing 16sRNA sequencing. Leaves extract of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis plant prepared in ethanol, methanol and aqueous solvents were tested against for antimicrobial activity. Results: Two multidrug resistance bacteria Kingella Sp. and Klebsiella sp. showed antibiotic resistant against more than three antibiotics sequenced for 16sRNA same compared and identified by phylogenetic tree analysis using MEGA 5.2 software. Methanolic extract of all three plant leaves showed comparatively good antimicrobial activity against two clinical isolates.

Keywords

Antimicrobial Activity, Woodfordia fruticosa, Adhatoda vasica, Ricinus communis, clinical Isolates, Kingella Sp., Klebsiella sp.
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  • Analysis of Antimicrobial Activity of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis Against Multi-Drug Resistant Bacteria

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Authors

Mahavir Joshi
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413, India
Sukhminderjit Kaur
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413, India
Monika Verma
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali, Punjab-140413, India
Tulika Mishra
School of Medicine, Xavier University, Santa Helenastraat 23, Oranjestad, Aruba, Dutch Caribbean, Aruba

Abstract


Introduction: Emergence of antibiotic resistance bacteria is one of major global problem and researcher are all focused to treat infection caused by antibiotic bacteria. Plants are used for their medicinal property from the ancient time. Plants are rich source of many phytoconstituents that do not possess any side effect. Material and methods: For the present work multidrug resistance bacteria were isolated from hospital soil sample and checked for antibiotic resistance by using antibiotic disk. The microorganisms showing resistant against more than three antibiotics were selected and identified by sequencing 16sRNA sequencing. Leaves extract of Woodfordia fruticosa, Adhatoda vasica and Ricinus communis plant prepared in ethanol, methanol and aqueous solvents were tested against for antimicrobial activity. Results: Two multidrug resistance bacteria Kingella Sp. and Klebsiella sp. showed antibiotic resistant against more than three antibiotics sequenced for 16sRNA same compared and identified by phylogenetic tree analysis using MEGA 5.2 software. Methanolic extract of all three plant leaves showed comparatively good antimicrobial activity against two clinical isolates.

Keywords


Antimicrobial Activity, Woodfordia fruticosa, Adhatoda vasica, Ricinus communis, clinical Isolates, Kingella Sp., Klebsiella sp.

References