Open Access
Subscription Access
Open Access
Subscription Access
Invention of an Anticancer Moiety from Indian River Spinach using Pharmaceutical Analytical Techniques
Subscribe/Renew Journal
Introduction: Since to meet the worlds challenging diseases, there is a huge requirement for finding remedies. The remedy might be derived from any source like synthetic, mineral, biological, microbiological, marine and herbal. Among which, herbal, the natural remedy stands first to treat an illness without side effects. The active secondary metabolites have been spread widely across the world. The herb that acts both as nutraceutical and as pharmaceutical majorly is various species of spinach. Hence, the water spinach is selected for getting an excellent moiety by means of different analytical procedures. Aim and Objectives: The aim is to characterize isolated herbal compound from Ipomoea aquatica Forsskal with the use of spectroscopic and chromatographic techniques. Method: The authenticated herb was extracted for its active metabolites. The screened phytochemicals by preliminary identification tests and TLC were applied for isolation. The high yield isolate from water fraction was evaluated for characterizing its chemical and biological properties. The structural elucidation was done with FTIR, 1H NMR and LCMS. The biological property was analyzed by colorimetric method using MCF-7 cell lines. Results and Discussion: The yield of isolate was 13.9 %w/w. The compound was characterized as halo aromatic alcoholamide with molecular weight of 1677.60 g/mol. IC50 value of active molecule by MTT assay was detected as 173.1 μg/ml. Conclusion: The invented anticancer natural drug might be isolated in bulk to prepare herbal formulations. The medicine could be utilized for treating various life threatening diseases because of its functional groups and pharmacological actions.
Keywords
Isolation, MCF-7, Water Spinach, FTIR, LCMS, NMR.
Subscription
Login to verify subscription
User
Font Size
Information
- Sasikala M, Mohan S, Ajitha Vasudevan Nambiar, Dona S, Karpakavalli M and Sundaraganapathy R. High Performance Thin Layer Chromatographic Determination of Phytoconstituents in Hydroalcoholic and Methanolic Whole Plant Extract of Peristylus goodyeroides (D. Don) Lindl. World J Pharma Res. 2017, 6 suppl 10: 1405-16.
- Sasikala M, Mohan S, Haritha T H, Manoj K, Karpakavalli M and Sundaraganapathy R. Extraction and Phytochemical Screening of Whole Plant of Peristylus goodyeroides (D. Don) Lindl. from Southern Whestern Ghats of India. World J Pharma Res. 2017, 6 suppl 10: 1394-404.
- Sasikala M and Sundaraganapathy R. Optimization of Extraction Method for Ipomoea aquatica Forssk. [Indian River Spinach] From Its Whole Plant, Int J Rec Sci Res, 2017, 8 suppl 5: 16967-971.
- Sasikala M, Sundara Ganapathy R, Karpakavalli M and Mohan S. Chemotaxonomical and Pharmacological Review on Medicinal Plants in Temperate Region, Int J Pharm and Tech, 2017, 9 suppl 1: 29165-172.
- Sasikala M and Sundaraganapathy R. Qualitative Analysis of Alkaloids Exist in the Hydroalcoholic Extract of Ipomoea aquatica Forssk. in Tamil Nadu. Int J Chem Tech Res, 2017, 10 suppl 7: 446-54.
- Sasikala M and Sundaraganapathy R. Fluorescence Detection of Phytoconstituents in Hydroalcoholic Extract of Ipomoea aquatica Forssk. by Thin Layer Chromatographic Analysis, Int J Pharmacog and Phytochem Res, 2017, 9 suppl 7: 990-6.
- Sasikala M and Sundaraganapathy R. Preliminary Phytochemical Evaluation of Hydroalcoholic Extract of Ipomoea Aquatica Forssk. from Aliyar Riverinein South India, Int J Pharma Bio Sci, 2017, 8 suppl 3: 356-65.
- Sasikala M, Karpakavalli M, Ranjithkumar D, Prakash G, Vanathi P and Mohan S. In-vitro Free Radical Scavenging Activity Studies of Extracts and Isolated Compounds of Eugenia jambolana Lam. Seeds, World J Pharma Res. 2016, 5 suppl 6: 2021-30.
- A.H. Beckett and J.B. Stenlake. Practical pharmaceutical chemistry. Thin layer chromatography. CBS Publishers. 4th Edition. 2005: 115-28.
- S.S. Khadabadi, S.L. Deore and B. A. Baviskar. Experimental Phytopharmacognosy – A Comprehensive guide. Nirali Prakashan, Pune, Maharashtra. Second edition, 2013. p.3.1-3.13, A 1.1-2.8.
- Harborne JB. Phytochemical Methods. Chapman and hall Ltd., London: U.K., 1973. 49-188.
- Y.R. Sharma, Elementary Organic Spectroscopy: Principles and Chemical Applications, S. Chand Publishers, Mumbai, 2010.
- Gunti Gowtham Raj, Hyma Sara Varghese, Sarita Kotagiri, B. M. Vrushabendra Swamy, Archana Swamy and Rafi Khan Pathan. Anticancer Studies of Aqueous Extract of Roots and Leaves of Pandanus odoratissimus f. ferreus (Y. Kimura) Hatus: An In Vitro Approach. J Tradit Complement Med. 2014 Oct-Dec; 4 suppl 4: 279–84.
- Sumitra Chanda and Krunal Nagani. In vitro and in vivo Methods for Anticancer Activity Evaluation and Some Indian Medicinal Plants Possessing Anticancer Properties: An Overview. J Pharmacog and Phytochem. 2013, 2 suppl 2:140-52.
- P.A. Shah, J.M. Parekh, P.S. Shrivastav. Assessment of critical extraction and chromatographic parameters for the determination of Bupropion and its three primary metabolites in human plasma by LC-MS/MS. Microchem. J., 2017, 135: 81-90.
- Kumaran S, Nazeema Banu B, Julie J, Abirami J, Kumareasan R, Muthukumaran T, Rajasree S and Jeya Jothi K. Anti-cancer activity of Datura metel in MCF-7 cell line. Asian J Pharma and Clini Res. 2014, 7 suppl 1: 181-183.
- Mukundam Borah, Swarnamoni Das and Shagufa Ahmed. Antibacterial activity of the ethanolic extract of leaves of Citrus maxima (Burm.) Merr. on Escherichia coli and Pseudomonas aeruginosa. Asian J Pharma and Clini Res. 2013, 6 suppl 4: 136-139.
Abstract Views: 319
PDF Views: 0