Research Journal of Pharmacy and Technology

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Anti-Mullerian Hormone and Vitamin D as a Predictor of Ovarian Reserve and Ovarian Response in Infertile Women undergoing IVF


Affiliations
1 Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq
     

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Aim: This study was aimed to measure AMH and 25(OH)D as a predictor of ovarian reserve among infertile females undergoing IVF in Iraq. Objective: To estimate anti-mullerian hormone as a predictor of fertility potential in terms of ovarian reserve and ovarian response reflected by antral follicles and mature oocyte counts in response to stimulation in in vitro fertilization (IVF) females. Materials and Methods: This prospective cohort study consisted of 60 women (mean age 26.67 years) undergoing IVF at Al-Amal Fertility Center in Baghdad. Blood withdrawal for Anti-mullerian hormone, vitamin D, FSH and E2 measurement was performed in all the patients and the number of oocytes were recorded. Results: Results showed that the ovarian response was better with younger age (<4, 4-8, 9-16 and >16 There was a significant positive association between ovarian response in terms of total number of oocytes and AMH levels (<4, 4-8, 9-16 and >16 oocytes there was no association between ovarian response in terms of total number of oocytes and vitamin D, FSH and E2 Moreover, Correlation coefficient revealed that the number of mature oocytes showed strong positive correlation with the AMH levels (r=5.27, p=0.001). The total number of oocytes was inversely associated with age There was a significant positive association between ovarian response in terms of the total number of oocytes and AMH levels there was no association between ovarian response in terms of the total number of oocytes with 25(OH)D FSH and E2 implying that AMH can be used as a good predictor of ovarian reserve and ovarian response. Conclusion: Anti-mullerian hormone can be used in IVF programs as a good predictor of ovarian reserve and ovarian response.

Keywords

Anti-Mullerian Hormone, Ovarian Reserve, IVF, Vitamin D, Infertility.
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  • Cate L, Mattaliano J, Hession C, Tizard R, Farber M, Cheung A, et al. Isolation of the bovine and human genes for Mullerian inhibiting substance and expression of the human gene in animal cells. Cell 1986; 45: 685-698.

  • Wunder M, Bersinger A, Yared M, Kretschmer R, Birkhauser H. Statistically significant changes of anti m mullerian hormone and inhibin levels during the physiologic menstrual cycle in reproductive age women. Fertil Steril. 2008; 89: 927-933.

  • Firouzabadi R, Tayebi N, Asgharnia M. Serum Level of Anti-Mullerian Hormone in Early Follicular Phase as a Predictor of Ovarian Reserve and Pregnancy Outcome in Assisted Reproductive Technology Cycles. Arch Iran Med. 2008; 11: 371-376

  • Freiesleben L, Rosendahl M, Johannsen T, Lossl K, Loft A, Bangsboll S, et al. Prospective investigation of serum anti-Mullerian hormone concentration in ovulatory intrauterine insemination patients: a preliminary study Reproductive. Biomed. 2010; 20: 582-587.

  • La Marca A, Giulini S, Tirelli A, Bertucci E, Marsella T, Xella S, et al. Anti-Mullerian hormone measurement on any day of the menstrual cycle strongly predicts ovarian response in assisted reproductive technology. Hum Reprod. 2006; 22: 766-771.

  • Durlinger L, Kramer P, Karels P, Hoogerbrugges W, van Leeuwen C. Endocrinology. 1999; 140: 5789-5796.

  • Jorgensen N, Rajpert-De Meyts E, Graem N, Muller J, Cate R, Skakkebaek N. Expression of anti-Mullerian hormone during normal and pathological gonadal development: association with differentiation of Sertoli and granulosa cells. Clin Endocrinol Metabol. 1999; 84: 3836-3844.

  • Lee M, Donahoe K. Mullerian inhibiting substance: a gonadal hormone with multiple functions. Endocrine Rev. 1993; 14: 152-164.

  • Lerchbaum E, Obermayer-Pleitsch B. Mechanisms in endocrinology: vitamin D and fertility: a systematic review. Eur J Endocrinol. 2012, 166:765–78.

  • Bikle D. Vitamin D metabolism, mechanism of action, and clinical applications. Chem Biol. 2014, 21:319–29.

  • Irani M, Merhi Z. Role of vitamin D in ovarian physiology and its implication in reproduction: a systematic review. Fertil Steril. 2014, 102:460–8.

  • Kebapcilar AG, Kulaksizoglu M, Kebapcilar L, Gonen MS, Unl€u A, Topcu A, et al. Is there a link between premature ovarian failure and serum concentrations of vitamin D, zinc, and copper? Menopause. 2012, 20:94–9.

  • Sun W, Xie H, Ji J, Zhou X, Goltzman D, Miao D. Defective female reproductive function in 1, 25(OH)2D-deficient mice results from indirect effect mediated by extracellular calcium and/or phosphorous. Am J Physiol Endocrinol Metab. 2010, 299: E928–35.

  • Chu J, Gallos I, Tobias A, Tan B, Eapen A, Coomarasamy A. Vitamin D and assisted-reproductive treatment outcome. Hum Reprod. 2018, 33:65–80.

  • Merhi Z, Seifer DB, Weedon J, Adeyemi O, Holman S, Anastos K, et al. Circulating vitamin D correlates with serum antimullerian hormone levels in later productive-aged women: Women’s Interagency HIV Study. Fertil Steril. 2012, 98:228–34.

  • Ombelet W., Cooke I., Dyer S., Serour G., Devroey P. Infertility and the provision of infertility medical services in developing countries. Hum Reprod Update. 2008, 14(6): 605-21.

  • Wang J, Sauer MV. In vitro fertilization (IVF): A review of 3 decades of clinical innovation and technological advancement. Ther Clin Risk Manag.2006;2(4):355-64.

  • Nugent D, Vandekerckhove P, Hughes E, et al. Gonadotrophin therapy for ovulation induction in subfertility associated with polycystic ovary syndrome. Cochrane Database Syst Rev. 2000; 4: CD000410.

  • Dehghani-Firouzabadi R, Tayebi N, Asgharnia M. Serum Level of Anti-mullerian Hormone in Early Follicular Phase as a Predictor of Ovarian Reserve and Pregnancy Outcome in Assisted Reproductive Technology Cycles. Arch Iran Med. 2008; 11: 4.

  • Van Rooij A, Broekmans J, Te Velde R, Fauser C, Bancsi F, De Jong H, et al. Serum anti-Mullerian hormone levels: a novel measure of ovarian reserve. Hum Reprod. 2002; 17: 3065-3071.

  • Seifer D, Mac Laughlin D, Christian B, Feng B, Shelden R. Early follicular serum Mullerian-inhibiting substance levels are associated with ovarian response during assisted reproductive technology cycles. Fertil Steril. 2002; 77: 468-471.

  • La Marca A, Spada E, Grisendi V, Argento C, Papaleo E, Milani S, et al. Normal serum anti-Müllerian hormone levels in the general female population and the relationship with reproductive history. Obstet Gynecol Reprod Biol. 2012; 163: 180-184.

  • Tolikas A, Tsakos E, Gerou S, Prapas Y, Loufopoulos A. Anti-Mullerian Hormone (AMH) levels in serum and follicular fluid as predictors of ovarian response in stimulated (IVF and ICSI) cycles. Hum Fertil (Cambridge). 2011; 14: 246-253.

  • Jeppesen JV, Anderson RA, Kelsey TW, Christiansen SL, Kristensen SG, Jayaprakasan K, et al. Which follicles make the most anti-Mullerian hormone in humans? Evidence for an abrupt decline in AMH production at the time of follicle selection. Mol Hum Reprod. 2013,19(8):519–27.

  • Hehenkamp WJ, Looman CW, Themmen AP, de Jong FH, TeVelde ER, Broekmans FJ. Anti-Mullerian hormone levels in the spontaneous menstrual cycle do not show substantial fluctuation. J Clin Endocrinol Metab. 2006, 91(10):4057–63.


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  • Anti-Mullerian Hormone and Vitamin D as a Predictor of Ovarian Reserve and Ovarian Response in Infertile Women undergoing IVF

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Authors

Hiba H. Kadhim
Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq
Salman A. Ahmed
Department of Chemistry, College of Science, Al-Nahrain University, Baghdad, Iraq

Abstract


Aim: This study was aimed to measure AMH and 25(OH)D as a predictor of ovarian reserve among infertile females undergoing IVF in Iraq. Objective: To estimate anti-mullerian hormone as a predictor of fertility potential in terms of ovarian reserve and ovarian response reflected by antral follicles and mature oocyte counts in response to stimulation in in vitro fertilization (IVF) females. Materials and Methods: This prospective cohort study consisted of 60 women (mean age 26.67 years) undergoing IVF at Al-Amal Fertility Center in Baghdad. Blood withdrawal for Anti-mullerian hormone, vitamin D, FSH and E2 measurement was performed in all the patients and the number of oocytes were recorded. Results: Results showed that the ovarian response was better with younger age (<4, 4-8, 9-16 and >16 There was a significant positive association between ovarian response in terms of total number of oocytes and AMH levels (<4, 4-8, 9-16 and >16 oocytes there was no association between ovarian response in terms of total number of oocytes and vitamin D, FSH and E2 Moreover, Correlation coefficient revealed that the number of mature oocytes showed strong positive correlation with the AMH levels (r=5.27, p=0.001). The total number of oocytes was inversely associated with age There was a significant positive association between ovarian response in terms of the total number of oocytes and AMH levels there was no association between ovarian response in terms of the total number of oocytes with 25(OH)D FSH and E2 implying that AMH can be used as a good predictor of ovarian reserve and ovarian response. Conclusion: Anti-mullerian hormone can be used in IVF programs as a good predictor of ovarian reserve and ovarian response.

Keywords


Anti-Mullerian Hormone, Ovarian Reserve, IVF, Vitamin D, Infertility.

References