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A New Pharmacological Model for Hepatocellular Carcinoma in Channa Punctatus and its Pharmaco Networking Studies


Affiliations
1 Amity Institute of Pharmacy, Amity University, NOIDA, India
2 Amity Institute of Biotechnology, Amity University, NOIDA, India
3 Department of Pharmaceutical Chemistry, Jamia Hamdard, Hamdard University, New Delhi, India
     

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Thioacetamide (TAA) is a well-known reported carcinogen for the induction of liver cancer. Different animal models have been developed to investigate different stages of HCC in rats, mice and guinea pigs. Use of these animals for new chemical entity leads to large number of deaths, sufferings and pain. Hence to reduce the number of animals while screening new moieties, use of fishes for establishing a diseased model is a novel and better concept. In the present study we report a Novel protocol for the induction of hepatocellular carcinoma in Channa Punctatus. After induction of TAA in the span of 21 days, different stages of HCC were observed. Moreover it was also found that AST, ALT and ALP levels were in elevated state. Further pharmaconetworking studies were established using STRING and it was found that important biological processes are disturbed in the presence of Thioacetamide.

Keywords

Thiacetamide, Hepatocellular Carcinoma, Channa Punctatus, STRING.
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  • A New Pharmacological Model for Hepatocellular Carcinoma in Channa Punctatus and its Pharmaco Networking Studies

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Authors

Shikha Saxena
Amity Institute of Pharmacy, Amity University, NOIDA, India
Sohini Singh
Amity Institute of Biotechnology, Amity University, NOIDA, India
Sandhya Bawa
Department of Pharmaceutical Chemistry, Jamia Hamdard, Hamdard University, New Delhi, India
Deepshikha Pande Katare
Amity Institute of Biotechnology, Amity University, NOIDA, India

Abstract


Thioacetamide (TAA) is a well-known reported carcinogen for the induction of liver cancer. Different animal models have been developed to investigate different stages of HCC in rats, mice and guinea pigs. Use of these animals for new chemical entity leads to large number of deaths, sufferings and pain. Hence to reduce the number of animals while screening new moieties, use of fishes for establishing a diseased model is a novel and better concept. In the present study we report a Novel protocol for the induction of hepatocellular carcinoma in Channa Punctatus. After induction of TAA in the span of 21 days, different stages of HCC were observed. Moreover it was also found that AST, ALT and ALP levels were in elevated state. Further pharmaconetworking studies were established using STRING and it was found that important biological processes are disturbed in the presence of Thioacetamide.

Keywords


Thiacetamide, Hepatocellular Carcinoma, Channa Punctatus, STRING.

References