Research Journal of Pharmacy and Technology

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In-vitro Cytotoxicity Assay of Betel Quid Extract Against HeLa Cells


Affiliations
1 Department of Biochemistry, School of Life Sciences, VISTAS, Chennai, 600117, Tamil Nadu,, India
     

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HeLa cells are broadly used for in-vitro studies in bringing out the medicinal value of plants against some dreadful diseases. From time immemorial, it is well proven that plant extracts play a vital role in treating many diseases including cancer. The plant extract contains copious varied phenolic compounds and flavonoids that are majorly responsible for its medicinal properties. In our study we have focused on the extraction of betel quid (betel leaves, areca nut and slaked lime in an appropriate proportion) using the solvent hexane. The hexane extract of the betel quid was evaluated for in vitro studies of apoptosis of HeLa cells. The results revealed that hexane extract of the betel quid at the maximum concentration of 200 μg/mL was very effective and only 20.89 % of cells were viable. The cytotoxic effect against HeLa cells was significant with IC50 of 96.33μg/ml. Further studies can be done on concentrating the bioactive compounds responsible for the anticancer activity. The bioactive active compounds can be formulated in the treatment of cancer in the upcoming research.

Keywords

HeLa cells, Betel quid, Cytotoxic effect, Anticancer.
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  • Xiaomei MA, Herbertn YU. Global burden of cancer. Yale J Biol Med. 2006; 79(3-4): 85-94.

  • Unnati S, Ripal S, Sanjeev A, Niyati A. Novel anticancer agents from plant sources. Chinese Journal of Natural Medicines. 2013; 11(1): 16-23.

  • Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 coubtries. CA Cancer J Clin. 2018; 68: 394-424.

  • Scherer WF, Syverton JT, Gey GO. Studies on the propagation in vitro of poliomyelitis viruses IV.Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. Journal of Experimental Medicine 1953; 97(5): 695-710.

  • Rutugandha P, Sushma RG, Lakshminarayana N, Arpana S, Ghazia A, Anjali P, Ritu A. Piper betel leaf extract: Anticancer benefits and bio-guided fractionation to identify active principles for prostate cancer management. Carcinogenesis. 2013; 34(7): 1558-1566.

  • Mehrtash H, Duncan K, Parascandola M, David A, Gritz ER, Gupta Pc, Wen CP. Defining a global research and policy agenda for betel quid and areca nut. The Lancet Oncology 2017; 18(12): 767-775.

  • Nasimun Islam NI, Jemimah Naine S, Shaik Jameel SS, Venkat Kumar, Mohanasrinivasan V, Subathra Devi C, Panneerselvam A. Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells. Research J. Pharm. and Tech. 2014; 7(5): 521-525.

  • Adhithya Ragunathan, Lokesh Ravi, Kannabiran Krishnan. Anticancer Cytotoxic Activity of Pentane-2,4-dione extracted from the Leaves of Cordia sebestena. Research J. Pharm. 2018; 11(6): 2191-2196.

  • Rutuja Sawant, Aloka Baghkar, Sanjukta Jagtap, Lina Harad, Anagha Chavan, Nilofar A. Khan, Rupali P. Yevale, Mohan K. Kale. A Review on - Herbs in Anticancer. Asian J. Res. Pharm. Sci. 2018; 8(4):179-184.

  • Poonam Gupta, Mohd. Yaqub Khan, Vikas Kumar Verma, Ashish Pathak. Beating Cancer with Natural Plant Sources. Asian J. Pharm. Tech. 2013; 3(2): 39-44.

  • Prafulla Sabale, Arjun Modi, Vidya Sabale. Curcuma longa Linn. A Phytochemical and Phytopharmacological Review. Research J. Pharmacognosy and Phytochemistry 2013; 5(2): 59-68.

  • Nagaveni P, Krishna Mohan G, Switi BG. Anticancer activity of maytenus emarginata leaves and stems: A comparative study. Research Journal of Pharmacognosy and Phytochemistry. 2015; 7(2): 101-106.

  • Tugba AF, Kargoz A, Ozcan G et.el. Invitro anticancer cytotoxic activities of some plant extracts on HeLa and Vero cell lenes. JBUON. 2016; 21(3): 720-725.

  • Mukesh Kumar D. J., Sonia K., Madhan R., Selvakumar K., Kalaichelvan P.T. Antiyeast, Antioxidant and Anticancer Activity of Tribulus terrestris Linn and Bougainvillea spectabilis Linn. Research J. Pharm. and Tech. 2011; 4(9): 1483-1489.

  • Manoj S. Pagare, Hardik Joshi, Leena Patil, Vilasrao J. Kadam. Human Milk: Excellent Anticancer Alternative. Research J. Pharm. and Tech. 2012; 5(1): 14-19.

  • Akshara Ravi, Gokul Raj M, Sathiavelu Arunachalam, Mythili Sathiavelu. Marine environment: A potential source for anticancer drugs. Research J. Pharm. and Tech. 2017; 10(5): 1543-1550.

  • Prakash SS, Piyush MP. Invitro biological screening of Avicennia marina for anticancer activity. Research Journal of Pharmacognosy and Pharmodynamics. 2013; 5(5): 297-301.

  • Baviskar HP, Dhake GT, Kasai MA, Chaudhari NB, Deshmukh TA. Review of Piper Betle. Res. J. Pharmacognosy and Phytochem. 2017; 9(2): 128-134.

  • Neeraj S Vyawahare, Virendra G Kagathara, RG Katedeshmukh, Pushpendra K Sharma, Smeeta M Mohod. Evaluation of Antiulcer Activity of Piper betel Leaves Extract in Rats. Research J. Pharmacology and Pharmacodynamics. 2010; 2(4): 278-282.

  • Kangralkar VA, Kulkarni AR. Evaluation of effect of Piper betel, Centella asiatica and Aristolochia indica extracts on bacterial enzymes in 1, 2-dimethyl hydrazine induced colon cancer in wistar rats. Research J. Pharm. and Tech. 2014; 7(2): 151-154.

  • Lipinska N, Romaniuk A, Paszel-Jaworska A, Toton E, Kopczynski P, Rubis B. Telomerase and drug resistance in cancer. Cell Mol Life Sci. 2017; 74(22): 4121-4132. doi:10.1007/s00018-017-2573-2.

  • Ganesan K, Xu B. Telomerase Inhibitors from Natural Products and Their Anticancer Potential. Int J Mol Sci. 2017; 19(1): 13. Published 2017 Dec 21. doi:10.3390/ijms19010013

  • Sari LM, Subita GP, Auerkari EI. Areca nut extract demonstrated apoptosis-inducing mechanism by increased caspase-3 activities on oral squamous cell carcinoma. F1000Res. 2018; 7: 723. Published 2018 Jun 11. doi:10.12688/f1000research.14856.5


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  • In-vitro Cytotoxicity Assay of Betel Quid Extract Against HeLa Cells

Abstract Views: 151  |  PDF Views: 0

Authors

B. Usharani
Department of Biochemistry, School of Life Sciences, VISTAS, Chennai, 600117, Tamil Nadu,, India
R. Monisha
Department of Biochemistry, School of Life Sciences, VISTAS, Chennai, 600117, Tamil Nadu,, India

Abstract


HeLa cells are broadly used for in-vitro studies in bringing out the medicinal value of plants against some dreadful diseases. From time immemorial, it is well proven that plant extracts play a vital role in treating many diseases including cancer. The plant extract contains copious varied phenolic compounds and flavonoids that are majorly responsible for its medicinal properties. In our study we have focused on the extraction of betel quid (betel leaves, areca nut and slaked lime in an appropriate proportion) using the solvent hexane. The hexane extract of the betel quid was evaluated for in vitro studies of apoptosis of HeLa cells. The results revealed that hexane extract of the betel quid at the maximum concentration of 200 μg/mL was very effective and only 20.89 % of cells were viable. The cytotoxic effect against HeLa cells was significant with IC50 of 96.33μg/ml. Further studies can be done on concentrating the bioactive compounds responsible for the anticancer activity. The bioactive active compounds can be formulated in the treatment of cancer in the upcoming research.

Keywords


HeLa cells, Betel quid, Cytotoxic effect, Anticancer.

References