Research Journal of Pharmacy and Technology

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Formulation and Evaluation of Patchouli Oil Microemulsion and Microemulgel for Inhibit the Bacterial Growth


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1 Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera,, Indonesia
     

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Patchouli oil from patchouli leaves (Pogostemon cablin, Benth) is known as the antimicrobial effect. Patchouli oil has been formulated into a gel dosage form. But the appearance of the gel could be improved to microemulsion and microemulgel. The aims of this study are to formulate the patchouli oil to microemulsion and microemulgel form and to determine the effect of the different dosage form to the ability of the patchouli oil to inhibit bacterial growth. Patchouli oil has been formulated to microemulsion (M) and microemulgel (MG) with three different concentrations i.e., 2%, 4%, and 6%. Evaluation of the resulting formulas included examining its general appearance, homogeneity, pH, stability test, spreadability test, and inhibition of bacterial growth. The results showed that patchouli oil microemulsion thinner than patchouli oil microemulgel. Microemulsion and microemulgel dosage forms were not affecting the ability of patchouli oil to inhibit of Citrobacter diversus and Staphylococcus .aureus bacterial growth.

Keywords

Patchouli (Pogostemon cablin) oil; microemulsion; microemulgel; Citrobacter diversus; Staphylococcus .aureus.
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  • Chakrapani, P., K. Venkatesh, S.S.B. Chandra, A.J. B., P. Kumar, A. P., and A.R. Rani, Phytochemical, Pharmacological Importance of Patchouli (Pogostemon cablin (Blanco) Benth) an Aromatic Medicinal Plant. Int. J. Pharm. Sci. Rev. Res., 2013; 21(2): 7-15.

  • van Beek, T.A. and D. Joulain, The essential oil of patchouli, Pogostemon cablin: A review. Flavour Fragr. J., 2017: 1-46.

  • Guenther, E., The essential oils. Vol. 1. 1948, New York: Van Nostrand Company Inc.

  • Bhuiyan, M.N.I., V.K. Varshney, S.C. Varshney, A. Tomar, and F. Akter, Composition of essential oil of the leaf and inflorescence of Pogostemon benghalensis (Burm.f.) Kuntze. International Research Journal of Plant Science, 2011; 2(9): 271-275.

  • Shigwan, A.V., A.B. Khade, B.C. Hatpakki, and S.M. Ghurghure, A Comprehensive Review on Pogostemon benghalensis (Burm. F.) O. Kuntze. Research and Reviews Journal of Pharmacognosy and Phytochemistry, 2013; 1(1): 10-15.

  • Kongkathip, N., P. Sam-ang, B. Kongkathip, Y. Pankaew, M. Tanasombat, and P. Udomkusonsri, Development of patchouli extraction with quality control and isolation of active compounds with antibacterial activity. Journal Kasetsart, 2009; 41: 519-525.

  • Karimi, A., Characterization and antimicrobial activity of patchouli essential oil extracted from Pogostemon cablin (Blanco) Benth. (Lamiaceae). Advances in Environmental Biology, 2014; 8(7): 2301-2309.

  • Febriyenti, Putri, R.F., Suharti, N., Formulation and Evaluation of Patchouli Oil Gel for Burn Wound, Jurnal Sains Farmasi dan Klinis, 2019; 6(3): 191-194.

  • Lokhande SS. Microemulsions as Promising Delivery Systems: A Review. Asian J Pharm Res. 2019;9(2):90-6.

  • Sahoo CK, Rao SRM, Sudhakar M, Hema. Challenges of Micro-Emulsion as a Novel Carrier for Drug Delivery. Res J Pharma Dosage Forms and Tech. 2019;11(3):227-34.

  • Mandavi N, Ansari N, Bharti R, Kader NSA, Sahu GK, Sharma H. Microemulsion: A Potential Novel Drug Delivery System. Res J Pharm Dosage Form and Tech. 2018;10(4):266-71.

  • Jagtap SR, Phadtare DG, Saudagar RB. Microemulsion: A Current Review. Res J Pharm Dosage Form and Tech 2016;8(2):161-70.

  • Mehta DP, Rathod HJ, Shah DP, Shah CN. A Review on Microemulsion Based Gel: A Recent Approach for Topical Drug Delivery System. Research J Pharm and Tech. 2015;8(2):118-26.

  • Derle DV, BSH S, Yeole DR. Development and Evaluation of Topical Microemulsion Gels for Protein and Peptide Drug Bacitracin Zinc. Research J Pharma Dosage Forms and Tech. 2009;1(3):217-21.

  • Priani, S.E., Darijanto, S.T., Suciati, T., Iwo, M.I., Formulasi Sediaan Emulgel untuk Penghantaran Trandermal Ketoprofen, Acta Pharmaceutica Indonesia, 2013; 38(1): 37-42.

  • Gulati, R., Sharma, S., and Gupta, V., Pharmacokinetcs of cyclosporine from conventional and new microemulsions formulation healthy volunteers. Journal of Association of Physicians of India, 2002; 46(10): 860-863.

  • Jadhav, K.R., Shetye, S.L., and Kadam, V.J. Design and evaluation of microemulsion based drug delivery system. International Journal of Advances In Pharmaceutical Sciences, 2010; 0976-1055, 156-166.

  • Hema, T.A., A.S. Arya, S. Subha, C.R.K. John, and P.V. Divya, Antimicrobial activity of five south indian medicinal plants against clinical pathogens. Int. J. Pharm. Bio. Sci., 2013; 4(1): 70-80.

  • Swarbrick, J. and J.C. Boylan, Encyclopedia of Pharmaceutical Technology. 1992, Marcel Dekker, Inc.: New York.

  • USP, The United States Pharmacopeia XXX - The National Formulary XXV. 2007, United States Pharmacopeial Convention, Inc.: Rockville.

  • Misal, G., G. Dixit, and V. Gulkari, Formulation and evaluation of herbal gel. Indian Journal of Natural Products and Resources, 2012; 3(4): 501 - 505.

  • Shivhare, U.D., K.B. Jain, V.B. Mathur, K.P. Bhusari, and A.A. Roy, Formulation development and evaluation of diclofenac sodium gel using water soluble polyacrylamide polymer. Digest Journal of Nanomaterials and Biostructures, 2009; 4(2): 285 - 290.

  • Febriyenti, Fitria, N., Mohtar, N., Umar, S., Noviza, D., Rineldi, S., Yunirwanti, Baie, S.B., Honey Gel and Film for Burn Wound, International Journal of Drug Delivery, 2014; 6: 1-6.

  • Katariya C, Arjunkumar R. Antimicrobial effect of curry leaves on Staphylococcus aureus – An In vitro Study. Research J Pharm and Tech. 2019;12(7):3318-22.

  • Markandeya AG, Firke NP, Gore SS, Gawali SS, Pingale SS. Antibacterial Activity of Celocia argentea Leaves Extract in Organic Solvents. Research Journal of Pharmacology and Pharmacodynamics. 2014;6(2):79-81.

  • Panwar AS, Upadhyay N, Bairagi M, Gujar S, Darwhekar GN, Jain DK, Emulgel: Review, AJPLS 2011,1: 333-343.

  • Islam, M.T., R.-H. N., S. Ciotti, and C. Ackermann, Rheological characterization of topical carbomer gels neutralized to different pH. Pharm. Res., 2004; 21(7): 1192-1199.

  • Hasçicek, C., A. Bediz-ÖLÇER, N. Gönül, Preparation and evaluation of different gel formulations for transdermal delivery of meloxicam. Turk. J. Pharm. Sci., 2009; 6(3): 177-186.

  • Wade, A. and J.W. Paul, eds. Handbook of Pharmaceutical Excipient. 2nd ed. 1994, The Pharmaceutical Press.: London.

  • Gupta S, Dhokne S, Verma R, Mitra M, Sahu U, Bhardwaj P, et al. Formulation aspects behind the development of a stable biphasic liquid dosage form with special reference to microemulsion. Research J Pharm and Tech. 2017;10(5):1509-16.

  • Ewing, W.H., and Davis, B.R. Biochemical characterization of Citrobacter diversus (Burkey) Werkman and Gillen and designation of the neotype strain. International Journal of Systematic Bacteriology, 1972; 22(1): 8-12.

  • Athanasopoulos, A.N., Economopolou, M., Orlova, V.V., Sobke, A., Schneider, D., Weber, H., Agustin, H.G., Eming, S.A., Schubert, U., Linn, T., Nawroth, P.P., Hussain, M., Hammes, H.P., Herman, M., Preissner, K.T., and Chavakis, T. The extracellular adherence protein of staphylococcus aureus inhibit wound healing by interfering with host defense and repair mechanisms. Blood Journal, 2005; 107: 1528-0020, 2720-2727.

  • Palmer, S.A., Steward J., and Eyfe, L. Antimicrobial properties of plant essential oil and assences againts five important food-borne pathogens. Left Applied Microbial, 1998; 26: 112-118.

  • Shahid SM, Umar N. Spectrum of Antimicrobial Susceptibility of E. coli and Staphylococcus aureus Isolates from Clinical Samples. Research J Pharm and Tech 2015;8(10):1399-402.

  • Min, D., Cheng, P., Feng, W., and Fu P. Antibacterial activity and mechanism of Pogostemon cablin against bacteria from milk of diary cows suffering with mastitis. Journal of Animal and Veterinary Advances. 2012;11(18): 1680-5593, 3289-3297.


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  • Formulation and Evaluation of Patchouli Oil Microemulsion and Microemulgel for Inhibit the Bacterial Growth

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Authors

Febriyenti
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera,, Indonesia
Netty Suharti
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera,, Indonesia
Rizki Febrika Putri
Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera,, Indonesia

Abstract


Patchouli oil from patchouli leaves (Pogostemon cablin, Benth) is known as the antimicrobial effect. Patchouli oil has been formulated into a gel dosage form. But the appearance of the gel could be improved to microemulsion and microemulgel. The aims of this study are to formulate the patchouli oil to microemulsion and microemulgel form and to determine the effect of the different dosage form to the ability of the patchouli oil to inhibit bacterial growth. Patchouli oil has been formulated to microemulsion (M) and microemulgel (MG) with three different concentrations i.e., 2%, 4%, and 6%. Evaluation of the resulting formulas included examining its general appearance, homogeneity, pH, stability test, spreadability test, and inhibition of bacterial growth. The results showed that patchouli oil microemulsion thinner than patchouli oil microemulgel. Microemulsion and microemulgel dosage forms were not affecting the ability of patchouli oil to inhibit of Citrobacter diversus and Staphylococcus .aureus bacterial growth.

Keywords


Patchouli (Pogostemon cablin) oil; microemulsion; microemulgel; Citrobacter diversus; Staphylococcus .aureus.

References