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A Study on Extraction and Characterization of Natural Red Pigment from a Soil Isolate Serratia Marcescens CN14


Affiliations
1 Marine Biotechnology laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
     

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Natural pigments are a brilliant alternative to artificial ones used for formulations in food, cosmetics and textile industries. Microorganisms, in particular, bacteria are an essentially obliging source for the production and extraction of biologically safe pigments. Thus, this study was aimed at isolating pigmented bacteria and extracting the pigment, characterizing it thereafter. Red pigmented bacteria isolated from a soil sample from the agricultural field of VIT, Vellore was used for the present study. The maximum growth was attained at room temperature. The bacterium was isolated and incubated for pigment production. The pigment was obtained from the pellets extracted using acetone and the purified pigment was identified by spectral analysis, TLC, HPLC and GCMS. The red color pigment, prodigiosin is considered as a potential treatment due to the activities against pathogens, the ability to control cancer can be an immunosuppressive and anti-proliferative agent. This gram-negative bacterium was identified as a Serratia marcescens strain by 16S rRNA gene sequencing. The gene sequence was submitted to NCBI as Serratia marcescens CN14 with an accession number of MT007931.

Keywords

Serratia marcescens, Prodigiosin, TLC, GCMS, HPLC, 16S rRNA gene sequence.
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  • Vora JU, Jain NK, Modi HA. Extraction , Characterization and Application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil. Int J pure Appl Biosci. 2014;2(6):160-168.

  • Abdelhafez AA, Husseiny SM, Abdel-Aziz Ali A, Sanad HM. Optimization of β-carotene production from agro-industrial by-products by Serratia marcescens ATCC 27117 using Plackett–Burman design and central composite design. Ann Agric Sci. 2016;61(1):87-96. doi:10.1016/j.aoas.2016.01.005

  • Lin C, Jia X, Fang Y, et al. Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets. Electron J Biotechnol. 2019;40:58-64. doi:10.1016/j.ejbt.2019.04.007

  • Arivizhivendhan K V., Boopathy R, Maharaja P, Regina Mary R, Sekaran G. Bioactive prodigiosin-impregnated cellulose matrix for the removal of pathogenic bacteria from aqueous solution. RSC Adv. 2015;5(84):68621-68631. doi:10.1039/c5ra09172a

  • Giri A V., Anandkumar N, Muthukumaran G, Pennathur G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol. 2004;4:1-10. doi:10.1186/1471-2180-4-11

  • Elkenawy NM, Yassin AS, Elhifnawy HN, Amin MA. Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation. Biotechnol Reports. 2017;14(October 2016):47-53. doi:10.1016/j.btre.2017.04.001

  • Malarvizhi A. Isolation, Identification, Optimization of Prodigiosin Pigment Produced by Serratia Marcescens and its Applications. WwwIjlemrCom ||. 2017;02(January):11-21. www.ijlemr.com.

  • Brass HUC, Klein AS, Nyholt S, Classen T, Pietruszka J. Condensing Enzymes from Pseudoalteromonadaceae for Prodiginine Synthesis. Adv Synth Catal. 2019;361(11):2659-2667. doi:10.1002/adsc.201900183

  • Namazkar S, Garg R, Ahmad WZ, Nordin N. Production and Characterization of Crude and Encapsulated Prodigiosin Pigment. 2013;4(3):116-129.

  • Nerurkar M, Vaidyanathan J, Adivarekar R, Langdana ZB. Use of a natural dye from serratia marcescens subspecies marcescens in dyeing of textile fabrics. Octa J Environ Res. 2013;1(2):129-135.

  • Rakh RR, Dalvi SM, Musle BB, Raut LS. Production, Extraction and Characterization of Red Pigment Produced by Serratia rubidaea JCM 1240T isolated from Soil. Int J Curr Microbiol Appl Sci. 2017;6(1):143-154. doi:10.20546/ijcmas.2017.601.018

  • Hassan A, Faraag I, El-Hendawy HH, Faraag AH, Ahmed ;, El-Batal I. Characterization of prodigiosin produced by Serratia marcescens strain isolated from irrigation water in Egypt combating Multi Drug Resistant Bacteria (MDRB) View project Molecular characterization of nano-conjugated antibiotics and its in silico mode of . Nat Sci. 2017;15(5). doi:10.7537/marsnsj150517.08

  • Tejasvini S. Pore, Ashwini B. Khanolkar NHN. Production, Purification, Identification of Prodigiosin From Serratia Sp. and Its Antimicrobial Activity. Res J Life Sci Bioinformatics, Pharm Chem Sci. 2016;1(325):212-221. doi:10.1161/CIRCULATIONAHA.115.020627

  • Stankovic N, Radulovic V, Petkovic M, et al. Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. Appl Microbiol Biotechnol. 2012;96(5):1217-1231. doi:10.1007/s00253-012-4237-3

  • De Araújo HWC, Fukushima K, Takaki GMC. Prodigiosin production by serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules. 2010;15(10):6931-6940. doi:10.3390/molecules15106931

  • Mishra S, Singh BR, Singh A, Keswani C, Naqvi AH, Singh HB. Biofabricated silver nanoparticles act as a strong fungicide against Bipolaris sorokiniana causing spot blotch disease in wheat. PLoS One. 2014;9(5). doi:10.1371/journal.pone.0097881

  • Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013;21(2):143-152. doi:10.1016/j.jsps.2012.05.002

  • Shaikh Z. Biosynthesis of Prodigiosin and Its Applications. IOSR J Pharm Biol Sci. 2016;11(6):01-28. doi:10.9790/3008-1106050128

  • Nsayef Muslim DS, Abbas Dham Z, J. Mohammed DN. Synthesis and characterization of nanoparticles conjugated tannase and using it for enhancement of antibacterial activity of tannase produced by Serratia marcescens. Microb Pathog. 2017;110:484-493. doi:10.1016/j.micpath.2017.07.024

  • Williams RH. The identification of prodigiosin and similar compounds. 1965:91.

  • Ibrahim D, Nazari TF, Kassim J, Lim SH. Prodigiosin - an antibacterial red pigment produced by Serratia marcescens IBRL USM 84 associated with a marine sponge Xestospongia testudinaria. J Appl Pharm Sci. 2014;4(10):1-6. doi:10.7324/JAPS.2014.40101


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  • A Study on Extraction and Characterization of Natural Red Pigment from a Soil Isolate Serratia Marcescens CN14

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Authors

K V Bhaskara Rao
Marine Biotechnology laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
Namratha K
Marine Biotechnology laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India
Cinchana K
Marine Biotechnology laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India

Abstract


Natural pigments are a brilliant alternative to artificial ones used for formulations in food, cosmetics and textile industries. Microorganisms, in particular, bacteria are an essentially obliging source for the production and extraction of biologically safe pigments. Thus, this study was aimed at isolating pigmented bacteria and extracting the pigment, characterizing it thereafter. Red pigmented bacteria isolated from a soil sample from the agricultural field of VIT, Vellore was used for the present study. The maximum growth was attained at room temperature. The bacterium was isolated and incubated for pigment production. The pigment was obtained from the pellets extracted using acetone and the purified pigment was identified by spectral analysis, TLC, HPLC and GCMS. The red color pigment, prodigiosin is considered as a potential treatment due to the activities against pathogens, the ability to control cancer can be an immunosuppressive and anti-proliferative agent. This gram-negative bacterium was identified as a Serratia marcescens strain by 16S rRNA gene sequencing. The gene sequence was submitted to NCBI as Serratia marcescens CN14 with an accession number of MT007931.

Keywords


Serratia marcescens, Prodigiosin, TLC, GCMS, HPLC, 16S rRNA gene sequence.

References