Research Journal of Pharmacy and Technology

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Evaluation the Antioxidant Enzymes Activity in Adults Male Rats Treated with Some New 3-mercapto1,2,4-triazole Derivatives


Affiliations
1 Biochemistry and Pharmacology Department, Veterinary Medicine College, Al-Qasim Green University, Babylon, Iraq
2 College of Pharmacy, University of Babylon, Babylon,, Iraq
3 College of Science, Department of Chemistry, Mustansiyriah University, Baghdad,, Iraq
     

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Three 1,2,4-triazole derivatives B, D, and E were evaluated their effect on the activity of antioxidant enzymes glutathione peroxidase (GPX) and superoxide dismutase (SOD) in vivo serum and liver injury in mice that exposed to thioacetamide. Male rats of the present experiment were randomly divided into six equal groups. First group (C-) the animals were received normal saline as a negative control. Other five groups: C+ and T1-T4 exposed to oxidative stress by thioacetamide 100 mg/kg. The four animals' groups T1, T2, T3 and T4 were received thioacetamide 100 mg/kg and treated orally with 0.21 mg/kg daily with ascorbic acid (A), compound B, compound D and compound E, respectively. The experiment was carried out for eight weeks. The results indicated that the tested compounds exhibited remarkable antioxidant activity. The highest activity of SOD enzyme values was recorded of compound D 2665 IU/L compared to ascorbic acid as a standard antioxidant agent 1657 IU/L. On the other-hand the increasing in the activity of GPX enzyme value was recorded after administration of compound D 2010 IU/L compared to ascorbic acid as a reference antioxidant agent 1682 IU/L at the same conditions. Significant differences in the responses of antioxidant enzymes to the different types of tested compounds were probably due to by the variant number and site of functional group in structure of studied compounds. The results suggested that alteration in enzymes activities may be applicable to the capacity of the liver and other inspected organs to cope with oxidative stress poisoned thioacetamide. The results of current study concluded that compounds B and D appeared clear improvement in scavenging activity to modulate toxicity of thioacetamide and regeneration of hepatocyte as well as normalized body function. Altogether, the results that were obtained from the present study could lead to design of new potent molecules via development of them in future studies.

Keywords

Triazole, Mercapto, Antioxidant, Glutathione peroxidase, Oxidative stress.
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  • Roesslein, M., et al., Comparability of in vitro tests for bioactive nanoparticles: a common assay to detect reactive oxygen species as an example. International Journal of Molecular Sciences, 2013. 14(12): p. 24320-24337.

  • Tan, B.L., et al., Antioxidant and oxidative stress: a mutual interplay in age-related diseases. Frontiers in Pharmacology, 2018. 9: p. 1162.

  • Kurutas, E.B., The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal, 2015. 15(1): p. 1-22.

  • Patil, V.P., et al., Facile preparation of tetrahydro-5H-pyrido [1, 2, 3-de]-1, 4-benzoxazines via reductive cyclization of 2-(8-quinolinyloxy) ethanones and their antioxidant activity. Bioorganic and Medicinal Chemistry Letters, 2013. 23(23): p. 6259-6263.

  • Azam, F., Therapeutic potential of free radical scavengers in neurological disorders. Handbook of free radicals: formation, types and effects. New York: Nova Publishers, 2010: p. 57-97.

  • Liguori, I., et al., Oxidative stress, aging, and diseases. Clinical Interventions in Aging, 2018. 13: p. 757.

  • Sindhi, V., et al., Potential applications of antioxidants–A review. Journal of Pharmacy Research, 2013. 7(9): p. 828-835.

  • Danta, C.C. and P. Piplani, The discovery and development of new potential antioxidant agents for the treatment of neurodegenerative diseases. Expert Opinion on Drug Discovery, 2014. 9(10): p. 1205-1222.

  • Khan, I., et al., Synthesis, antioxidant activities and urease inhibition of some new 1, 2, 4-triazole and 1, 3, 4-thiadiazole derivatives. European Journal of Medicinal Chemistry, 2010. 45(11): p. 5200-5207.

  • Zafar, S., R. Ahmed, and R. Khan, Biotransformation: a green and efficient way of antioxidant synthesis. Free Radical Research, 2016. 50(9): p. 939-948.

  • Li, S., et al., The role of oxidative stress and antioxidants in liver diseases. International Journal of Molecular Sciences, 2015. 16(11): p. 26087-26124.

  • Ariffin, A., et al., PASS-assisted design, synthesis and antioxidant evaluation of new butylated hydroxytoluene derivatives. European Journal of Medicinal Chemistry, 2014. 87: p. 564-577.

  • Mansour, H.M., et al., The anti-inflammatory and anti-fibrotic effects of tadalafil in thioacetamide-induced liver fibrosis in rats. Canadian Journal of Physiology and Pharmacology, 2018. 96(12): p. 1308-1317.

  • Bashandy, S.A., et al., Role of zinc oxide nanoparticles in alleviating hepatic fibrosis and nephrotoxicity induced by thioacetamide in rats. Canadian Journal of Physiology and Pharmacology, 2018. 96(4): p. 337-344.

  • Xie, Y., et al., Cytochrome P450 dysregulations in thioacetamide-induced liver cirrhosis in rats and the counteracting effects of hepatoprotective agents. Drug Metabolism and Disposition, 2012. 40(4): p. 796-802.

  • Luo, M., et al., Protective effects of pentoxifylline on acute liver injury induced by thioacetamide in rats. International Journal of Clinical and Experimental Pathology, 2015. 8(8): p. 8990.

  • Sies, H., Oxidative stress: a concept in redox biology and medicine. Redox Biology, 2015. 4: p. 180-183.

  • Jasim, A.M., H.F. Hasan, and M.J. Awady, Preparation of Vorapaxar loaded with Vitamin E TPGS and PVA emulsified PLGA nanoparticles In vitro studies. Research Journal of Pharmacy and Technology, 2019. 12(9): p. 4503-4510.

  • Al-Mansury, Sadiq, et al. Synthesis, Antiproliferative and Antioxidant Activity of 3-Mercapto-1, 2, 4-Triazole Derivatives as Combretastatin A-4 Analogues. Pharmaceutical Chemistry Journal. 2021. 55(6): 556-565.

  • Kirtawade, R., et al., Herbal antioxidant: Vitamin C. Research Journal of Pharmacy and Technology, 2010. 3(1): p. 58-61.

  • Yaroshenko, A.I., T.V. Panasenko, and Y.S. Pruhlo, Impact of substituents of various nature at C-3 and C-5 of 4H-1, 2, 4-Triazole on compounds’behavior under the conditions of GC-MS analysis. Journal of Chemistry and Technologies, 2019. 27(2): p. 264-275.

  • Al Diab, D. and N. Al Asaad, Comparative analysis of ascorbic acid content and antioxidant activity of some fruit juices in Syria. Research Journal of Pharmacy and Technology, 2018. 11(2): p. 515-520.

  • Adikwu, E., B. Nelson, and O.W. Atuboyedia, Melatonin and alpha lipoic acid as possible therapies for lopinavir/ritonavir-induced hepatotoxicity in albino rats. Physiology and Pharmacology, 2016. 20(4).

  • Zelko, I.N., T.J. Mariani, and R.J. Folz, Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression. Free Radical Biology and Medicine, 2002. 33(3): p. 337-349.

  • Niloufer, S. and L.B. Lakshmi, In-vitro Analysis of Phytochemical, Anti-oxidant capacity of seed Ethanolic extracts of Sapindus saponaria Vahl and Anti-bacterial activity on Common Dental Pathogens. Research Journal of Pharmacy and Technology, 2021. 14(1): p. 351-355.

  • Gatellier, P., Y. Mercier, and M. Renerre, Effect of diet finishing mode (pasture or mixed diet) on antioxidant status of Charolais bovine meat. Meat Science, 2004. 67(3): p. 385-394.

  • Kaushik, S. and J. Kaur, Chronic cold exposure affects the antioxidant defense system in various rat tissues. Clinica Chimica Acta, 2003. 333(1): p. 69-77.

  • Jasim, A.M., et al., Characterization and Synthesis of Selenium-TPGS Nanoparticles for Target Delivery Clove to Minimize Cytogenic and Liver Damage Induced in Adult Male Rats. Nano Biomed. Eng, 2021. 13(2): p. 127-136.

  • Srinath, A., V. Jyothi, and V. Jyothi, Hepatoprotective activity-a review. International Journal of Pharmacy and Technology, 2010. 2(3): p. 354-366.

  • Jasim, A., A. Dirwal, and A. Al-Yassri, Role of zontivity loaded by PLGA coated by TPGS in ameliorating induced cardiovascular and pulmonary disorder in adult male. East African Medical Journal, 2019. 96(6).

  • Kadir, F.A., et al., Effect of Tinospora crispa on thioacetamide-induced liver cirrhosis in rats. Indian Journal of Pharmacology, 2011. 43(1): p. 64.

  • Salama, S.M., et al., Mechanism of hepatoprotective effect of Boesenbergia rotunda in thioacetamide-induced liver damage in rats. Evidence-Based Complementary and Alternative Medicine, 2013. 2013.

  • Bashandy, S.A., et al., Potential effects of the combination of nicotinamide, vitamin B2 and vitamin C on oxidative-mediated hepatotoxicity induced by thioacetamide. Lipids in Health and Disease, 2018. 17(1): p. 29.

  • Jasim, A.M. and H.F. Hasan, Evaluation of Vorapaxar and TPGS-PLGA loading Vorapaxar to reduced apoptosis and liver damage in atherosclerosis male rats. Iraqi National Journal of Chemistry, 2019. 19(1).

  • Jiang, G.-w., et al., Preparation and antitumor effects of 4-amino-1, 2, 4-triazole Schiff base derivative. Journal of International Medical Research, 2020. 48(2): p. 0300060520903874.


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  • Evaluation the Antioxidant Enzymes Activity in Adults Male Rats Treated with Some New 3-mercapto1,2,4-triazole Derivatives

Abstract Views: 94  |  PDF Views: 0

Authors

Sadiq Al-Mansury
Biochemistry and Pharmacology Department, Veterinary Medicine College, Al-Qasim Green University, Babylon, Iraq
Mohammed A. Aboktifa
Biochemistry and Pharmacology Department, Veterinary Medicine College, Al-Qasim Green University, Babylon, Iraq
Adnan M Jassim
Biochemistry and Pharmacology Department, Veterinary Medicine College, Al-Qasim Green University, Babylon, Iraq
Asim A. Balakit
College of Pharmacy, University of Babylon, Babylon,, Iraq
Fatin Fadhel Alkazazz
College of Science, Department of Chemistry, Mustansiyriah University, Baghdad,, Iraq

Abstract


Three 1,2,4-triazole derivatives B, D, and E were evaluated their effect on the activity of antioxidant enzymes glutathione peroxidase (GPX) and superoxide dismutase (SOD) in vivo serum and liver injury in mice that exposed to thioacetamide. Male rats of the present experiment were randomly divided into six equal groups. First group (C-) the animals were received normal saline as a negative control. Other five groups: C+ and T1-T4 exposed to oxidative stress by thioacetamide 100 mg/kg. The four animals' groups T1, T2, T3 and T4 were received thioacetamide 100 mg/kg and treated orally with 0.21 mg/kg daily with ascorbic acid (A), compound B, compound D and compound E, respectively. The experiment was carried out for eight weeks. The results indicated that the tested compounds exhibited remarkable antioxidant activity. The highest activity of SOD enzyme values was recorded of compound D 2665 IU/L compared to ascorbic acid as a standard antioxidant agent 1657 IU/L. On the other-hand the increasing in the activity of GPX enzyme value was recorded after administration of compound D 2010 IU/L compared to ascorbic acid as a reference antioxidant agent 1682 IU/L at the same conditions. Significant differences in the responses of antioxidant enzymes to the different types of tested compounds were probably due to by the variant number and site of functional group in structure of studied compounds. The results suggested that alteration in enzymes activities may be applicable to the capacity of the liver and other inspected organs to cope with oxidative stress poisoned thioacetamide. The results of current study concluded that compounds B and D appeared clear improvement in scavenging activity to modulate toxicity of thioacetamide and regeneration of hepatocyte as well as normalized body function. Altogether, the results that were obtained from the present study could lead to design of new potent molecules via development of them in future studies.

Keywords


Triazole, Mercapto, Antioxidant, Glutathione peroxidase, Oxidative stress.

References