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Qualitative Phytochemical Screening and Effectiveness Analysis Of Batissa Violacea Celebensis Martens 1897 Crude Extract Against Antioxidant and Cytotoxic Activity


Affiliations
1 Doctoral Program, Graduate School of Medicine, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
2 Department of Biochemistry, Faculty of Mathematics and Natural Science, IPB University Bogor, Indonesia
3 Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
4 Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
5 Department of Internal Medicine, Division of Hematology Medical Oncology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
6 Department of Environmental Science, Faculty of Forestry and Environmental Science Halu Oelo University, Kendari southeast Sulawesi, Indonesia
     

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The aim of this study was to conduct phytochemical tests aimed at identifying the bioactive components present in the powder crude extract of pokea shell can be applied in analyzing antioxidant activity and testing cytotoxic activity. This study consists of phytochemical tests, including alkaloids, steroids, triterpenoids, saponins, and flavonoids. The antioxidant activity measured by the DPPH method from the crude extract of the pokea shell is still relatively weak but the crude extract of methanol of the boiled and dried pokea shells in toxic category so potentially as a cytotoxic compound. The result of phytochemical test of crude extract of pokea shell meat, is extract of fresh and boiled pokea shell contain flavonoid, saponin, steroid and triterpenoid while extract of dried pokea shell only contain saponin, steroid and triterpenoid. The result of the antioxidant activity test of crude extract of pokea shell shows that the crude extract of dried pokea shell has the highest value of antioxidant activity. The result of the toxicity test of crude extract pokea shell boiled and dried pokea shell have an LC50 value of less than 1000 ppm. These values indicate that the extract of the boiled and dried Pokea shell is showed potential for development as an alternative anticancer agents in the future.

Keywords

Phytochemical, Antioxidant, Cytotoxicity, Pokea shell, Crude extract.
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  • Qualitative Phytochemical Screening and Effectiveness Analysis Of Batissa Violacea Celebensis Martens 1897 Crude Extract Against Antioxidant and Cytotoxic Activity

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Authors

Sri Anggarini Rasyid
Doctoral Program, Graduate School of Medicine, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
Maria Bintang
Department of Biochemistry, Faculty of Mathematics and Natural Science, IPB University Bogor, Indonesia
Suryani As’ad
Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
Upik Miskad
Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar,, Indonesia
Rahmawati Minhajat
Department of Internal Medicine, Division of Hematology Medical Oncology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Ridwan Adi Surya
Department of Environmental Science, Faculty of Forestry and Environmental Science Halu Oelo University, Kendari southeast Sulawesi, Indonesia

Abstract


The aim of this study was to conduct phytochemical tests aimed at identifying the bioactive components present in the powder crude extract of pokea shell can be applied in analyzing antioxidant activity and testing cytotoxic activity. This study consists of phytochemical tests, including alkaloids, steroids, triterpenoids, saponins, and flavonoids. The antioxidant activity measured by the DPPH method from the crude extract of the pokea shell is still relatively weak but the crude extract of methanol of the boiled and dried pokea shells in toxic category so potentially as a cytotoxic compound. The result of phytochemical test of crude extract of pokea shell meat, is extract of fresh and boiled pokea shell contain flavonoid, saponin, steroid and triterpenoid while extract of dried pokea shell only contain saponin, steroid and triterpenoid. The result of the antioxidant activity test of crude extract of pokea shell shows that the crude extract of dried pokea shell has the highest value of antioxidant activity. The result of the toxicity test of crude extract pokea shell boiled and dried pokea shell have an LC50 value of less than 1000 ppm. These values indicate that the extract of the boiled and dried Pokea shell is showed potential for development as an alternative anticancer agents in the future.

Keywords


Phytochemical, Antioxidant, Cytotoxicity, Pokea shell, Crude extract.

References