Research Journal of Pharmacy and Technology

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Anti-hyperglycemic Effect of Polyherbal Formulation in Glucose Loaded and Epinephrine Induced Hyperglycemic Wistar Rats


Affiliations
1 Ph.D. Student, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri - 411018 Dist- Pune (M.S.), India
2 Professors, Dr. D. Y. Patil College of Pharmacy, Akurdi – 411035 Dist - Pune (M.S.), India
     

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The poly herbal plant extracts were efficient in reducing the elevated glucose level than the independent use of plants. The poly herbal formulation were containing the extracts of Syzgium cumini, Annona squamosa, Momordica charantia, Tinospora cordifolia, Gymnema sylvestre and Curcuma longa. This poly herbal formulation was studied for anti-hyperglycemic activity in the glucose loaded and epinepharine induced hyperglycemic rats. The animal were divided into 5 groups each group had 6 animals; group no 1 was positive control, group no2, 3, 4 were different dose of poly herbal formulation PHF 225 mg/kg, PHF 450 mg/kg and PHF 850 mg/kg respectively and group no 5 was treated by Metformin 500mg/kg. The rats were treated for a period of 7 days, blood glucose level and biochemical parameters were measured on 7thday. The polyherbal formulation significantly (p<0.01) reduced the elevated blood glucose level in epinephrine and glucose induced hyperglycemic rats when compared with positive control group. The PHF has significantly (p<0.05) lower HDL, VDL and cholesterol as compared with the positive control groups. The epinephrine is known to enhance glucagon secretion by α-cells of pancreas and activation of β receptors resulting in an increased glycogenolysis and gluconeogenesis, thereby increases blood glucose level. The active phyto-constituents present in poly herbal formulation are flavonoids and phenolic compounds, triterpene saponins known as gymnemic acids, gymnemasaponins promote the release of insulin and delay the absorption of glucose.

 


Keywords

Polyherbal formulation, epinephrine, Glucose, Gymnemic acids, anti-hyperglycemic.
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  • Uppala PK, Radhadevi JB, Atchuta K, Bangaruthalli K. Formulation and Evaluation of Mosquito Repellent Activity of Polyherbal Formulations of Extraction of Annonasquamosa, Azadirachtaindica, Eucalyptus alba, Citrus aurantium and Rosa indica and their phytochemical analysis. Asian Journal of Pharmaceutical Technology. 2017; 7(2): 91-102.

  • Mazumder A, Singh S, Chakaraborthy SG. Potential herbs against diabetes mellitus - an update. International Journal of Pharmaceutical Science and Research. 2019; 10: 3619 – 26.

  • Mali KK, Ligade SS, Dias RJ. Delaying Effect of Polyherbal Formulation on Cataract in STZ-NIC-induced Diabetic Wistar Rats. Indian Journal of Pharmaceutical Science. 2019; 81: 415-23.

  • Perera PR, Ekanayake S, Ranaweera KK. Antidiabetic Compounds in Syzygium cumini Decoction and Ready to Serve Herbal Drink. Evidence-Based Comp Alt Med. 2017; 1-5.

  • Kumar D, Mitra A, Manjunatha M. In vitro and in vivo studies of antidiabeticindian medicinal plants: a review. Journal of Herbal Medicine and Toxicology. 2009; 3: 10 – 17.

  • Kolhe SS, Punit R, Rachh PR. Review on potent anti-diabetic plants or herbs from traditional medicine. Journal of Drug Delivery and Therapeutics. 2018; 8: 92-98.

  • Goyal RK, Jain S, Dahima R. Indigenous Medicine used for Treatment of Diabetes in Gwalior Chambal Region of Madhya Pradesh. Research Journal of Pharmacy and Technology 6(5): May 2013; Page 589-591.

  • Singh j, Cumming E, Gunasekar M, Huba K, Adeghate E. Medicinal Chemistry of the Anti-diabetic effects of Momordica charantia: active constituents and modes of actions. The Open Medicinal Chemistry Journal. 2011; 5: 70-77.

  • Olatunde A, Joel EB, Tijjani H, Obidola SM, Luka CD. Anti-diabetic Activity of Aqueous Extract of Curcuma longa (Linn) Rhizome in Normal and Alloxan-Induced Diabetic Rats. Researcher. 2014; 6: 58-65.

  • Saha S, Ghosh S. Tinospora cordifolia One plant, many roles. Ancient Science of Life.2012; 31: 151 - 60.

  • Suraj J. Patil, Shivani D. Patil, Pratibha B. Patil, Pranali S. Patil, Ganesh B. Vambhurkar, Indryani D. Raut. Evaluation of Standardization Parameters of Ayurvedic Marketed Polyherbal Formulation. Asian J. Pharm. Ana. 2018; 8(4): 220-226. doi: 10.5958/2231-5675.2018.00040.6

  • Devi C, Ramesh B. Hypoglycemic activity of Leaves of Bougainvillea spectabilis extract in Streptozotocin-Induced Diabetic Rats. Asian Journal of Pharmaceutical Research. 2018; 8(2):99-103.

  • Arumugam G, Manjula P, Paari N. A review: Anti diabetic medicinal plants used for diabetes mellitus. Journal of Acute Diseases. 2013;2: 196- 00.

  • Revised Document (Dec-2001) OECD/OCDE 420. Guidelines on Acute Oral Toxicity. OECD Guidelines for the testing of chemicals, 1-14.

  • Laxmi V, Khatri A, Kaushik B, Patil UK, Pawar RS Antidiabetic activity of Cassia occidentalis (Linn) in normal and alloxan-induced diabetic rats. Indian Journal of Pharmacology. 2010; 42: 224-28.

  • Raut SP, Kar DM, Maharana L. Anti-hyperglycemic effect of different fractions of Annona reticulata leaf. Asian Journal of Pharmaceutical Clinical Research. 2016; 9: 256 - 62.

  • Nikhat F, Satyanarayana D, Shastri CS, Moid A, Thouheed A. Investigation of Phytoconstituents, from Momordicacharantia Linn Roots and Screening them, for Anti-Hyperglycemic Activity. Asian J. Research Chem. 7(3): March 2014; Page 256-261.

  • Naik SR, Mandalik RV, Desai SK. Antidiabetic activity of a polyherbal formulation (DRF/AY/5001). Indian Journal of Experimental Biology. 2008;46: 599 - 06.

  • Lopes MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clinical Chemistry. 1977; 23(5), 882-884.

  • McGowan MW, Joseph DA, Strandbergh DR, Zak B. A Peroxidasecoupled method for the colorimetric determination of Blood triglyceride. Clinical Chemistry. 1983; 538-542.

  • Zhang Z, Radziuk J. Inverse relationship between peripheral insulinremoval and action: studies with metformin. American Journal of Physiology Endocrinology and Metabolism. 2001; 281: 1240-48.

  • Eidi A, Eidi M, Esmaeili E. Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats. Phytomedicine. 2006; 13: 624-29.

  • Carson JF. Chemistry and biological properties of onion and garlic. Food Reviews International. 1987; 3:71– 03.

  • Patil PM, Chaudhari PD, Duragkar NJ, Katolkar PP. Formulation and Evaluation its Anti-diabetic Activity of Liquid Oral Preparation of Gymnema sylvestre and Stevia rebaudiana and their Combination in Alloxan Diabetic Rats. Research Journal of Pharmacy and Technology. 2010; 3(4): 1200-1204.

  • Manal HS, Eman MS, Mona MT,Gehan MM, Maha HM. Phyto-constituents from Curcuma longa L. aqueous ethanol extract and its immunomodulatory effect on diabetic infected rats. Egyptian Pharmaceutical Journal. 2015; 14: 36–43.

  • Tiwari P, Mishra BN, Sangwan NS. Phytochemical and Pharmacological Properties of Gymnema sylvestre: An Important Medicinal Plant.BioMed Research International. 2014; 1-18.

  • Ghorbani Z, Hekmatdoost A, Mirmiran P. Anti-hyperglycemic and insulin sensitizer effects of turmeric and its principle constituent curcumin. International Journal of Endocrinology and Metabolism.2014; 12: 18081.

  • Joseph B, Jini D. Antidiabetic effects of Momordicacharantia (bitter melon) and its medicinal potency. Asian Pacific Journal of Tropical Disease. 2013; 3(2): 93-102.

  • Meher N, Panda BB, Ray B. In Vivo Antidiabetic Activity of fruit extract of Cocciniagrandis Linn in Normoglycemic, Adrenaline Induced and Alloxan Induced Diabetic rat. Research Journal of Pharmacy and Technology. 2019; 12(12): 5917-5922.

  • Geerling JJ, Boon M, Gerard C. Metformin lowers plasma triglycerides by promoting VLDL-triglyceride clearance by brown adipose tissue in mice. American Diabetic Association Diabetes. 2014; 63: 880-891.


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  • Anti-hyperglycemic Effect of Polyherbal Formulation in Glucose Loaded and Epinephrine Induced Hyperglycemic Wistar Rats

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Authors

Vikas B Gawali
Ph.D. Student, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri - 411018 Dist- Pune (M.S.), India
Niraj S Vyawahare
Professors, Dr. D. Y. Patil College of Pharmacy, Akurdi – 411035 Dist - Pune (M.S.), India

Abstract


The poly herbal plant extracts were efficient in reducing the elevated glucose level than the independent use of plants. The poly herbal formulation were containing the extracts of Syzgium cumini, Annona squamosa, Momordica charantia, Tinospora cordifolia, Gymnema sylvestre and Curcuma longa. This poly herbal formulation was studied for anti-hyperglycemic activity in the glucose loaded and epinepharine induced hyperglycemic rats. The animal were divided into 5 groups each group had 6 animals; group no 1 was positive control, group no2, 3, 4 were different dose of poly herbal formulation PHF 225 mg/kg, PHF 450 mg/kg and PHF 850 mg/kg respectively and group no 5 was treated by Metformin 500mg/kg. The rats were treated for a period of 7 days, blood glucose level and biochemical parameters were measured on 7thday. The polyherbal formulation significantly (p<0.01) reduced the elevated blood glucose level in epinephrine and glucose induced hyperglycemic rats when compared with positive control group. The PHF has significantly (p<0.05) lower HDL, VDL and cholesterol as compared with the positive control groups. The epinephrine is known to enhance glucagon secretion by α-cells of pancreas and activation of β receptors resulting in an increased glycogenolysis and gluconeogenesis, thereby increases blood glucose level. The active phyto-constituents present in poly herbal formulation are flavonoids and phenolic compounds, triterpene saponins known as gymnemic acids, gymnemasaponins promote the release of insulin and delay the absorption of glucose.

 


Keywords


Polyherbal formulation, epinephrine, Glucose, Gymnemic acids, anti-hyperglycemic.

References