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Adjuvant Therapy of Syzygium Cumini Leaf and Fruit Extract Nanoparticles to Histopathological Changes of Mice Organ with Malaria


Affiliations
1 Department of Basic Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
2 Department of Veterinary Pathology, Universitas Airlangga, Surabaya,, Indonesia
3 Department of Veterinary Pathology, Universitas Airlangga, Surabaya, Indonesia
4 Department of Veterinary Microbiology, Universitas Airlangga, Surabaya,, Indonesia
5 5Department of Husbundary, Universitas Airlangga, Surabaya,, Indonesia
6 Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
     

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Plasmodium infection can cause serious complications such as damage to internal organs. The purpose of this study was determining the effect of nanoparticles adjuvant therapy of Syzygium cumini leaf and fruit extract to histopathological changes of mice organ with malaria. Methods: The study used 70 Swiss Albino mice, weighing 20g-30g, 2.5 months old. The study was divided into 7 treatment groups as follows K0 : not infected, K+: infected but untreated, K1: infected and treat with chloroquine, K2: infected and treated with Syzygium cumini leaf extract, K3: infected and treated with Syzygium cumini fruit extract, P1: infected and treated with nanoparticles Syzygium cumini leaf extract, P2: infected and treated with combination of nanoparticles leaf extract of Syzygium cumini and chloroquine, P3: infected and treated with nanoparticles Syzygium cumini fruit extract, P4: infected and treated with combination of nanoparticles fruit extract of Syzygium cumini and chloroquine. The infection dose was 1x10-5 Plasmodium berghei in 0.2 ml. Treatment was done for 4 days, 24 hours after infection. Data were analyzed by Kruskal Wallis and continued with the Mann Whitney test. The results showed that the most severe organ damage was in the K+ group and the lightest was the K0 group. The P2 and P4 groups showed a decrease in organ damage which was not significantly different from the K0 group (p> 0.05). Meanwhile, K +, K1, K2, K3, P1 and P3 groups showed significant differences (p> 0.05) with K0. Conclusion: nanoparticles extract of Syzygium cumini leaf and fruit as an adjuvant therapy can reduce liver, kidney, lung and brain damage of mice infected with Plasmodium berghei.

Keywords

Malarial Infection, Organ Damage, Nanoparticles, Syzygium cumini.
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  • Adjuvant Therapy of Syzygium Cumini Leaf and Fruit Extract Nanoparticles to Histopathological Changes of Mice Organ with Malaria

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Authors

Lilik Maslachah
Department of Basic Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
Thomas V Widiyatno
Department of Veterinary Pathology, Universitas Airlangga, Surabaya,, Indonesia
Nusdianto Triakoso
Department of Veterinary Pathology, Universitas Airlangga, Surabaya, Indonesia
Suwarno
Department of Veterinary Microbiology, Universitas Airlangga, Surabaya,, Indonesia
Koesnoto P
5Department of Husbundary, Universitas Airlangga, Surabaya,, Indonesia
Nanda Ayu Narulita
Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
Mahendra Pujiyanto
Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
Zerlinda Dyah Ayu
Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia
Dita Nurkurnia Putri
Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, Indonesia

Abstract


Plasmodium infection can cause serious complications such as damage to internal organs. The purpose of this study was determining the effect of nanoparticles adjuvant therapy of Syzygium cumini leaf and fruit extract to histopathological changes of mice organ with malaria. Methods: The study used 70 Swiss Albino mice, weighing 20g-30g, 2.5 months old. The study was divided into 7 treatment groups as follows K0 : not infected, K+: infected but untreated, K1: infected and treat with chloroquine, K2: infected and treated with Syzygium cumini leaf extract, K3: infected and treated with Syzygium cumini fruit extract, P1: infected and treated with nanoparticles Syzygium cumini leaf extract, P2: infected and treated with combination of nanoparticles leaf extract of Syzygium cumini and chloroquine, P3: infected and treated with nanoparticles Syzygium cumini fruit extract, P4: infected and treated with combination of nanoparticles fruit extract of Syzygium cumini and chloroquine. The infection dose was 1x10-5 Plasmodium berghei in 0.2 ml. Treatment was done for 4 days, 24 hours after infection. Data were analyzed by Kruskal Wallis and continued with the Mann Whitney test. The results showed that the most severe organ damage was in the K+ group and the lightest was the K0 group. The P2 and P4 groups showed a decrease in organ damage which was not significantly different from the K0 group (p> 0.05). Meanwhile, K +, K1, K2, K3, P1 and P3 groups showed significant differences (p> 0.05) with K0. Conclusion: nanoparticles extract of Syzygium cumini leaf and fruit as an adjuvant therapy can reduce liver, kidney, lung and brain damage of mice infected with Plasmodium berghei.

Keywords


Malarial Infection, Organ Damage, Nanoparticles, Syzygium cumini.

References