Research Journal of Pharmacy and Technology

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Phytochemical Screening and Antipyretic Activity of Atylosia Rugosa


Affiliations
1 Associate Professor, Department of Biotechnology, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India
2 Professor, MNR College of Pharmacy, Sangareddy, Hyderabad, 502001,, India
3 Associate Professor, Department of Pharmaceutical Management, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India
4 Associate Professor, Department of Pharmaceutics, Avanthi Institute of Pharmaceutical Sciences, Hayatnagar , Hyderabad, India
5 Associate Professor, Department of Pharmaceutical Analysis, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India
     

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In the present study, the ethanolic extract of Atylosia rugosa was investigated for antipyretic activity in rats by using brewers yeast induced pyrexia. The plant was collected from tirumala hills, dried and powdered, to about 1000gms. This powder was subjected to successive soxhlet extraction using petroleum ether, choloroform, acetone, ethylacetate and ethanol. In this study only ethanolic extract was subjected to antipyretic activity. The study was carried out in healthy wistar albino rats weighing about 150-200g, brewers yeast of 20% suspension of 20ml/kg in normal saline is used for inducing pyrexia for antipyretic activity. The animals were divided into five groups. Group I is treated with 2%v/v Tween80 (10ml/kg), Group II treated with Paracetamol 100mg/kg, Group III, IV, V treated with ethanolic extract of Atylosia rugosa 100mg/kg, 200mg/kg, 400mg/kg body weight respectively. Conclusion: A positive, significant linear relationship between antipyretic activity and the phytocontituents. The extract is rich of phenolic compounds and flavonoids. The present study revealed the significant antipyretic activity of the plant is because of its active metabolites in the plant in the Atylosia rugosa.

Keywords

Atylosia rugosa, Flavonoids, Brewers yeast, Paracetamol, Pyrexia.
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  • Hazeena Begum V, Velavan S. Lipids regulating activity of Asparagus racemosus root in young and aged rats. Indian Journal of Gerontology,2011; 25(1):273-285.

  • Joy PP, Thomas J, Mathew S. Medicinal plants. Tropical horticulture. Calcutta: Naya Prakash, 2001; 2(1):449-632.

  • Amos S, Kolawole E, Akah P. Behavioural effects of the aqueous extract of Guiera senegalensis in mice and rats. Phytomedicine, 2001; 8(2):356-361.

  • Ramarao AV, Gunjar MK. Drugs from plant resources an overview. Pharma Times,1990;22(1):19-27.

  • Chaudhri R.D. “Indian Herbal Drug Industry”, A Practical Approach to Industrial Pharmacognosy, 1st ed., Eastern Publishers, New Delhi, 1996; 45-49.

  • Kong JM, Goh N, Chin LS. and Chia TF. Recent advances in traditional plant drugs and orchids, Acta Pharmacol, 2003; 24, 7.

  • Rachana Mishra, D L Verma , Principal Antioxidative flavonoids from Rosmarinus officinalis, Grown in central Himalaya. Research J . Pharm.and Tech .2011;4(3):476-479.

  • Amit Tapas1, Dinesh Sakarkar and Rajendra Kakde. The Chemistry and Biology of Bioflavonoids. Research J. Pharm. and Tech. 2008; 1(3):132-143.

  • Grusak MA. Enhancing mineral content in plant food products. J Am Coll Nutr. 2002; 21(3): 178–183.

  • Grusak MA. Phytochemicals in plants: genomics-assisted plant improvement for nutritional and health benefits. Curr Opin Biotechnol. 2002;13(2):508-511.

  • Madar Z. and Stark AH. New legume sources as therapeutic agents. British Journal of Nutrition. 2002; 88(1):287–292.

  • Dixon RA, Achnine L, Kota P, Liu CJ, Reddy MSS. and Wang L.The phenylpropanoid pathway and plant defence: a genomics perspective. Molecular Plant Pathology. 2002;3(3): 371–390.

  • Ndakidemi PA, and Dakora FD. Review: legume seed flavonoids and nitrogenous metabolites as signals and protectants in early seedling development. Functional Plant Biology. 2003; 30(1): 729–745.

  • Andersen JW, Story L, Sieling B, Chen WJL, Petro MS and Story J. Hypocholesterolemic effects of oat-bran or bean intake for hypercholesterolemic men. American Journal of Clinical Nutrition. 1984; 40(1): 1146–1155.

  • Jenkins DJA, Kendall CWC, Marchie A, Jenkins AL, Augustin LSA, Ludwig DS, Barnard ND, Anderson JW. Type 2 diabetes and the vegetarian diet. American Journal of Clinical Nutrition. 2003; 78(3): 610–616.

  • Gupta YP. Anti-nutritional and toxic factors in food legumes: a review.Qual Plant Foods Hum Nutr. 1987; 37(1): 201–228.

  • Spergel JM, Fiedler JM. Natural history of peanut allergy. Curr Opin Pediatr. 2001; 13(2):517–522.

  • Hoga JC. Combinational chemistry in drug discovery. Journal of Biotechnology. 1997;15(1):328.

  • Chattopadhyay D, Arunachalam G, Ghosh L, Antipyretic activity of Alstonia macrophylla Wall ex A. DC: An ethnomedicine of Andaman Islands. J Pharm Pharmaceutical Sci. 2005; 8(1): 558 – 564.

  • Spacer CB, Breder CD, The neurologic basis of fever. N Engl J Med. 1994;330: (2) 1880 – 1886.

  • Veugelers PJ, Kaldor JM, Strathdee SA, Incidence and prognostic significance of symptomatic primary human immunodeficiency virus type 1 infection in homosexual men. J Infect Dis. 1997; 176: (1) 112 – 117.

  • Cheng L, Ming-liang H, Lars B. Is COX-2 a perpetrator or a protector? Selective COX-2 inhibiotrs remain controversial. Acta Pharmacologica Sinica. 2005;26(3):926-933.

  • B.S.Krishnamoorthy, N. Nattuthurai, Arunkumar K., Revathi T, S. Kamatchi. Preliminary Studies on Phytochemicals of Solvent Extracts of Eichhornia crassipes. Research J. Pharm. and Tech. 2014; 7(6): 635-637.

  • .Hayat M. Mukhtar, Poonam Wadhan and Varinderpal Singh, Pharmacognostical Characters of the Dried Flower Heads of Artemisia maritima Linn. Collected From Baramulla Region (Kashmir). Research J. Pharm. and Tech. 4(8):2011;1320-1322.

  • N. S. Bembde, P. V. Meshram, M. K. Patil, Junaid Shaikh, The Preliminary Phytochemical Analysis of Ethanolic extract of Tylophora indica. Research J. Pharmacognosy and Phytochemistry. 2020;12(1):01-06. 26. Kokate CK. Practical Pharmacognosy. 2nd edn; Vallabh Prakashan, Delhi.1988;125.

  • H.G.Vogel. Drug Discovery and Evaluation pharmacological assays, 2nd edition, Springer, Newyork. 2002; 716.

  • M. Umadevi, C. Maheswari , R. Jothi, Sai Kishore Paleti, Y. Srinivasa Reddy and R. Venkata Narayanan. Hepatoprotective Activity of Flowers of Madhuca longifolia (Koen.) Macbr. Against Paracetamol-Induced Hepatotoxicity. Research J. Pharm. and Tech. 2011; 4(2): 259-262.

  • Kumar S , Alagawadi KR. Hypoglycemic effect of Argyreria nervosa root extract in normal and streptozocin – diabetic rats. Der Pharmacia Letter. 2010; 2(1):337-337.

  • T. Nargis Begum , A. Vijaya Anand and R. Senthil. Antipyretic Activity of Azima tetracantha in Experimental Animals. Research J. Pharmacognosy and Phytochemistry. 2010; 2(6): 451-454

  • Ram Kumar Sahu, Amit Roy, Saurabh Kothiya, Anup Kumar Maurya, Rajesh Kumar. Screening of Antipyretic and Analgesic Potential of Ethanol Extract of Cassytha filiformis Leaves. Research J. Science and Tech. 2012; 4(3): 129-131.

  • Sudhish Rai, Ashish Vaishnav, Anish Chandy, Mamta Singh and M.P. Singh. Antipyretic activity of Ethanolic Extract of Premna corymbosa Rottl. Leaves (Verbenacea). Research J. Pharmacognosy and Phytochemistry. 2011; 3(6): 278-280

  • Vogel HG, Vogel WH, Scholkens BA, Sandow J, Mulle G, and Vogel WF. Drug discovery and evaluation. Pharmacological Assays. 2002; 388, 692, 725, 772, 817.

  • Smith PK, Hambourger WE. The ratio of the toxicity of acetanilide to its antipyretic activity in rats, J. Pharmacol. Exptl. Ther. 1935;54(2):346-349.

  • M. Sheeba Rani, S. Emmanuel and M. Raja Sreekanth, T Rosemary. Evaluation of the Antipyretic and Anti-inflammatory Activities of Methanolic Fraction and Chrysophenol of Cassia occidentalis Linn. Research J. Pharm. and Tech. 2010; 3(3): 888-893.

  • Chattopadhyay D, Arunachalam G, Ghosh L. J Pharm. and Pharma. Sci. 2005; 8(3):558-564

  • Ukwuani AN, Abukar MG, Wara SH, Antipyretic activity of some Nigerian medicinal plants in rats. Int J Pharm Res. 2012; 4(1): 48-51.

  • Gege-Adebayo GI, Bassi AS, Igbokwe VU, Antipyretic effect of Ocimum gratissium on brewers yeast induced fever in wistar rats. J Med Sci. 2013;4(1): 247-251.

  • Aman A A, Patrick N O. Investigation of anti-inflammatory, antipyretic and analgesic effect of Yemeni Sidr honey. World Acad Sci Eng Technol. 2011;80(1):47-52.

  • Zeisberger E, From humoral fever to neuroimmunological control of fever. J Therm Biol . 1999;24(3):287- 326.

  • Roth J. Endogenous antipyretics. Clin Chim Acta. 2006;371(1): 13-24.

  • Banks WA, Plotkin S R, Kastin AJ. Permeability of blood brain barrier to soluble cytokinine receptors. Neuro Immunomodulation. 1995;24(2)161-165.

  • Roth J, Harre EM, Rummel C. Signaling the brain in systemic inflammation role of sensory circumventricular organs. Front Biosci. 2004;9 (1):290-300.

  • Mastsumura K, Kobayashi S. Signaling the brain systemic inflammation the role of endothelial cells. Front Biosci. 2004; 9(1):2819-2826.

  • Schiltz JC, Sawchenko PE. Signaling the brain in systemic inflammation the role of perivascular cells. Front Biosci. 2003; 8(1):1321-1329.

  • Saper CB, Breder CD. The neurologic basis of fever. N Eng J Med.1994; 330(2):1880-1886.

  • KD Tripathi. Essentials of Medical pharmacology, 6th edition , Jaypee Brothers Medical Publishers Pvt. Ltd. 2008;188.

  • Pretorious JC, Antiinfective Agents . Current Medical Chemistry. 2003; 2(1):335-353.

  • Khan MR. The Indian Pharmacist. 2003; 69-72. 50. Tominaga K, Journal of Antimicrobial Chemotherapy. 2002; 50(1): 547-552.

  • Wagner H, Immunostimulants and adaptogens from plants. In: Amason JT, Mata R , and Romeo JT. Phytochemistry of Medicinal Plants. Plenum Press, Newyork. 1995;1-18.

  • Clark WO, Cumby HR. The antipyretic effect of Indomethacin. J Physiol.1975; 248(1):625-638.


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  • Phytochemical Screening and Antipyretic Activity of Atylosia Rugosa

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Authors

Prathibha M. Bharathi
Associate Professor, Department of Biotechnology, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India
Alagarsamy V
Professor, MNR College of Pharmacy, Sangareddy, Hyderabad, 502001,, India
Siva S. Prasad
Associate Professor, Department of Pharmaceutical Management, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India
Sadak C. Vali
Associate Professor, Department of Pharmaceutics, Avanthi Institute of Pharmaceutical Sciences, Hayatnagar , Hyderabad, India
Venkata Murali P. Krishna
Associate Professor, Department of Pharmaceutical Analysis, MNR College of Pharmacy, Sangareddy, Hyderabad, Telangana,, India

Abstract


In the present study, the ethanolic extract of Atylosia rugosa was investigated for antipyretic activity in rats by using brewers yeast induced pyrexia. The plant was collected from tirumala hills, dried and powdered, to about 1000gms. This powder was subjected to successive soxhlet extraction using petroleum ether, choloroform, acetone, ethylacetate and ethanol. In this study only ethanolic extract was subjected to antipyretic activity. The study was carried out in healthy wistar albino rats weighing about 150-200g, brewers yeast of 20% suspension of 20ml/kg in normal saline is used for inducing pyrexia for antipyretic activity. The animals were divided into five groups. Group I is treated with 2%v/v Tween80 (10ml/kg), Group II treated with Paracetamol 100mg/kg, Group III, IV, V treated with ethanolic extract of Atylosia rugosa 100mg/kg, 200mg/kg, 400mg/kg body weight respectively. Conclusion: A positive, significant linear relationship between antipyretic activity and the phytocontituents. The extract is rich of phenolic compounds and flavonoids. The present study revealed the significant antipyretic activity of the plant is because of its active metabolites in the plant in the Atylosia rugosa.

Keywords


Atylosia rugosa, Flavonoids, Brewers yeast, Paracetamol, Pyrexia.

References