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Anticancer Effect of Naringin on Human Colon Cancer (Widr Cells): In Vitro Study


Affiliations
1 Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, India
2 Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, Indonesia
3 Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, Indonesia
     

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Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80μg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14μg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.

Keywords

Naringin, Colon cancer, Caspase-3, Apoptosis.
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  • Anticancer Effect of Naringin on Human Colon Cancer (Widr Cells): In Vitro Study

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Authors

Amaq Fadholly
Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, India
Arif N. M. Ansori
Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, Indonesia
Budi Utomo
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, 60115,, Indonesia

Abstract


Naringin, a bioflavonoid naturally found in citrus species, has been exert as promising anticancer activities. However, naringin mechanism as anticancer still remain enigmatic. This study, WiDr cell lines as human colon cancer cell in vitro were used to evaluate anticancer effect of naringin. Naringin (10, 20, 40, 60, 80μg/mL) treated on WiDr cells to evaluate IC50 value by MTT assay, then continued with analyzed caspase-3 expressions (IC50, 2IC50) as apopotosis marker. This study reported that naringin inhibit the growth of WiDr cell with IC50 value is 63.14μg/mL. The caspase-3 expressions on 2IC50 is higher (74.66%) than IC50 (56.66%). These results indicate that naringin has properties of inhibting cell growth and inducing caspase-3 expressions on WiDr cells. It is a potential compound to be developed as a chemopreventive agent for colon cancer.

Keywords


Naringin, Colon cancer, Caspase-3, Apoptosis.

References