Research Journal of Pharmacy and Technology

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Determination of Anti-tuberculosis Activity of Psychotria Sarmentosa, Aponogeton Crispus and Two Species of Pleurotus Mushrooms


Affiliations
1 Postgraduate Institute of Science, University of Peradeniya,, Sri Lanka
2 Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura,, Sri Lanka
3 Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,, Sri Lanka
4 Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy,, Sri Lanka
     

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Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (Mtb) complex. The global TB epidemic has been aggravated by the emergence of disease outbreaks caused by multi-drug resistant and extensively drug-resistant strains. The aim of the present study was to evaluate the in-vitro, anti-TB activity of leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms Pleurotus ostreatus and Pleurotus cystidiosus found in Sri Lanka. Leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms; Pleurotus ostreatus and P. cystidiosus were dried until a constant weight and 120 g each were taken to prepare crude extracts with distilled water (1.9 L) by heating at a moderate temperature and the final volume was reduced to 240 ml. Freeze dried aqueous extracts were incorporated in Middle Brook 7H11 medium (1mg/ml) using pour plate method.Two ten-fold dilutions (10-2 and 10-4) of standard H37Rv Mtb suspensions were inoculated on Middle Brook 7H11 media with the crude extracts. The plates were incubated at 37 0C for 4 weeks until visible appearance of Mtb colonies. The inhibitory effect of each extract was calculated by the mean reduction of number of colonies on extract containing medium compared to extract-free control medium. Accordingly, the highest mean percentage inhibition was shown by P. sarmentosa (71.0 %). The mean percentage inhibition exerted by A.               crispus, P. ostreatus and P. cystidiosus were 46.0 %, 43.4 % and 39.5 % respectively. Therefore, freeze-dried aqueous extract from leaves of P. sarmentosa has certain activity against the tested standard mycobacterial strain and has a potential to be used as an anti-TB drug component.

Keywords

Psychotria sarmentosa, Aponogeton crispus, Pleurotus ostreatus, Pleurotus cystidiosus, Mycobacterium tuberculosis.
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  • Determination of Anti-tuberculosis Activity of Psychotria Sarmentosa, Aponogeton Crispus and Two Species of Pleurotus Mushrooms

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Authors

Jayamini Jayantha
Postgraduate Institute of Science, University of Peradeniya,, Sri Lanka
Banukie Jayasuriya
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura,, Sri Lanka
Dilanthi Herath
Department of Pharmacy and Pharmaceutical Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura,, Sri Lanka
Sugandhika Suresh
Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura,, Sri Lanka
Dhammika Magana-Arachchi
Molecular Microbiology and Human Diseases Project, National Institute of Fundamental Studies, Hantana Road, Kandy,, Sri Lanka

Abstract


Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (Mtb) complex. The global TB epidemic has been aggravated by the emergence of disease outbreaks caused by multi-drug resistant and extensively drug-resistant strains. The aim of the present study was to evaluate the in-vitro, anti-TB activity of leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms Pleurotus ostreatus and Pleurotus cystidiosus found in Sri Lanka. Leaves of Psychotria sarmentosa, Aponogeton crispus and the mushrooms; Pleurotus ostreatus and P. cystidiosus were dried until a constant weight and 120 g each were taken to prepare crude extracts with distilled water (1.9 L) by heating at a moderate temperature and the final volume was reduced to 240 ml. Freeze dried aqueous extracts were incorporated in Middle Brook 7H11 medium (1mg/ml) using pour plate method.Two ten-fold dilutions (10-2 and 10-4) of standard H37Rv Mtb suspensions were inoculated on Middle Brook 7H11 media with the crude extracts. The plates were incubated at 37 0C for 4 weeks until visible appearance of Mtb colonies. The inhibitory effect of each extract was calculated by the mean reduction of number of colonies on extract containing medium compared to extract-free control medium. Accordingly, the highest mean percentage inhibition was shown by P. sarmentosa (71.0 %). The mean percentage inhibition exerted by A.               crispus, P. ostreatus and P. cystidiosus were 46.0 %, 43.4 % and 39.5 % respectively. Therefore, freeze-dried aqueous extract from leaves of P. sarmentosa has certain activity against the tested standard mycobacterial strain and has a potential to be used as an anti-TB drug component.

Keywords


Psychotria sarmentosa, Aponogeton crispus, Pleurotus ostreatus, Pleurotus cystidiosus, Mycobacterium tuberculosis.

References