Research Journal of Pharmacy and Technology

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Effect of Amlodipine in Proliferative Retinopathy: Biochemical Estimation and Zebrafish (Danio Rerio) Modeling


Affiliations
1 School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, India
2 Neurobiology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, College Square, Cuttack, Odisha 753003,, India
3 Centurion University of Technology and Management, Odisha, India
4 Research Associate II Hetero Biopharm Unit III, Hyderabad, Telangana., India
     

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Proliferative retinopathy is described as a progressive neovascularization in the inner surface of the retina or vitreous, which subsequently threatens vision by causing retinal detachment or vitreous hemorrhage. Reactive oxygen species generation and its related vasoproliferative factor up regulation play a major role in progression of the disease. The present study focuses on Carr induced reactive species generation with its related vasoproliferative factor up regulation and ameliorative activity of AML. Biochemical estimation of catalase, lipid peroxidation, hypoxia inducing factor (HIF-1α) and guanyl-s-transferase (GST) were done. AML treated group showed significant increase in catalase enzyme, guanyl-s-transferase and significantly reduced the lipid peroxidation. Western blotting assay showed a significant decrease in HIF-1α in AML treated group. The progression of PR alters the endogenous cell signalling thus causing havoc in retinal cells. Therefore, it could be hypothesized that use of AML can be a new detection method for therapeutic strategies to prevent blindness in PR.

Keywords

Amlodipine, Proliferative retinopathy, Biochemical estimation, Hypoxia.
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  • Wang GL, Jiang BH, Rue EA, Semenza GL. 1995 HIF1 is a BHLH-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci USA. 92 (12): 5510-5514.

  • Hirota K, Semenza GL, Small GTPase protein Rac1 plays an essential role in hypoxia-inducible factor 1 activation in hypoxia. J Biol Chem. 2001; 276 (24): 21166 –21172.

  • Selman M. Selman Amlodipine Causes Ecchymosis and Hematuria, Research J. Pharm. and Tech. 2018; 11(9): 3799-3801.

  • Pant S, Pal K., Development and Validation of a Simultaneous HPLC Method for Assay and Dissolution of Bisoprolol fumarate and Amlodipine besylate in Pharmaceutical Dosage, Journal: Research Jr of Pharm Dosage Forms and Technology, 2012; 4(1):62-66.

  • Sharma D., Mehta D.K., Bhatti K., Das R., Chidurala R.M., Amlodipine And Atenolol: Combination Therapy Versus Monotherapy In Reducing Blood Pressure - A Focus On Safety And Efficacy, Research J. Pharm. and Tech. 2020; 13(6): 3007-3013.

  • Gandla K, Ravindra N., Jyothsna B., Development and Validation of a Reversed-Phase HPLC Method for Simultaneous Determination of Aspirin, Atenolol and Amlodipine in Capsules Dosage Form, Asian J. Pharm. Ana. 4(3): July-Sept. 2014; Page 116-120.

  • Mottet D, Michel G, Renard P, Ninane N, Raes M, Michiels C. Role of ERK and calcium in the hypoxia-induced activation of HIF-1. J Cell Physiol. 2003 ;194(1):30-44.

  • Imredy JP, Yue DT, Mechanism of Ca2+sensitive inactivation of L-type Ca2+ channels. Neuron. 1994 ; 12(6):1301-18.

  • Nanda A, Sahoo RN, Pramanik A, Mohapatra R, Pradhan SK, Thirumurugan A, Das D, Mallick S. Drug-in-mucoadhesive type film for ocular anti-inflammatory potential of amlodipine: Effect of sulphobutyl-ether-beta-cyclodextrin on permeation and molecular docking characterization, Colloids Surf B Biointerfaces. 2018; 172: 555-564

  • Bhattacharyya S, Dudeja PK, Tobacman JK. Carrageenan-induced NFkappaB activation depends on distinct pathways mediated by reactive oxygen species and Hsp27 or by Bcl10. Biochim Biophys Acta. 2008; 1780(7-8): 973-982.

  • Mohanty R., S.K. Das, N.R. Singh, M. Patri, Withania somnifera leaf extract ameliorates benzo[a]pyrene-induced behavioral and neuromorphological alterations by improving brain antioxidant status in zebrafish (Danio rerio). Zebrafish. 2016; 13 (3): 188-196.

  • Ohkawa, H., N. Ohishi, K. Yagi, Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Annals of Biochemistry. 1979; 95 (2): 351–358.

  • Girish P.K., Vamshi Krishna T., Lalit Kumar, M. Sreenivasa Reddy, G. Shreenidhi, A. Praneeth Reddy Comparative evaluation of the physical parameters of uncoated tablets of Amlodipine Besylate with different types of primary packaging materials, Asian J. Pharm. Res. 3(1): Jan.-Mar. 2013; Page 03-05.

  • Sarkar S., Mukherjee S., Chattopadhyay A., Bhattacharya S. Low dose of arsenic trioxide triggers oxidative stress in zebrafish brain: expression of antioxidant genes. Ecotoxicol Environ Saf. 2014; 107:1–8.

  • Massarsky A, Kozal JS, Di Giulio RT. Glutathione and zebrafish: Old assays to addres current issue. Chemosphere. 2017; 168: 707-715.

  • Sangarran D, Ramachandram, V. Chitra, R., Correlation of Anti-oxidant, Anti-angiogenic, and Cytotoxic activity of Tanacetum parthenium compared with Amlodipine using Chorioallantoic membrane Assay, Research J. Pharm. and Tech. 2020; 13(4):1665-1671.

  • Köblitz L, Fiechtner B, Baus K, Lussnig R, Pelster B. Developmental Expression and HIF Inducible Transcription Factors in the Zebrafish. PLoS One. 2015;10(6):e0128938. Published 2015 Jun 8. doi:10.1371/journal.pone.0128938.

  • Rawat R, Josh Y. Effect of Antihypertensive Drugs on Homocysteine level among Hypertensive Patients, Asian J. Res. Pharm. Sci. 2018; 8(4): 219-222.


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  • Effect of Amlodipine in Proliferative Retinopathy: Biochemical Estimation and Zebrafish (Danio Rerio) Modeling

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Authors

Ashirbad Nanda
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, India
Sai Aparna
Neurobiology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, College Square, Cuttack, Odisha 753003,, India
Rudra Narayan Sahoo
Centurion University of Technology and Management, Odisha, India
Arunima Pramanik
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, India
Bhargab Satpathy
Research Associate II Hetero Biopharm Unit III, Hyderabad, Telangana., India
Subrata Mallick
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, India
Debajyoti Das
School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, Odisha 751003,, India
Manorama Patri
Neurobiology Laboratory, Department of Zoology, School of Life Sciences, Ravenshaw University, College Square, Cuttack, Odisha 753003,, India

Abstract


Proliferative retinopathy is described as a progressive neovascularization in the inner surface of the retina or vitreous, which subsequently threatens vision by causing retinal detachment or vitreous hemorrhage. Reactive oxygen species generation and its related vasoproliferative factor up regulation play a major role in progression of the disease. The present study focuses on Carr induced reactive species generation with its related vasoproliferative factor up regulation and ameliorative activity of AML. Biochemical estimation of catalase, lipid peroxidation, hypoxia inducing factor (HIF-1α) and guanyl-s-transferase (GST) were done. AML treated group showed significant increase in catalase enzyme, guanyl-s-transferase and significantly reduced the lipid peroxidation. Western blotting assay showed a significant decrease in HIF-1α in AML treated group. The progression of PR alters the endogenous cell signalling thus causing havoc in retinal cells. Therefore, it could be hypothesized that use of AML can be a new detection method for therapeutic strategies to prevent blindness in PR.

Keywords


Amlodipine, Proliferative retinopathy, Biochemical estimation, Hypoxia.

References