Research Journal of Pharmacy and Technology

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A Taxonomic Study Comparing the Two Types of Medicinal Leeches Available in Iraq


Affiliations
1 College of Pharmacy, University of Mosul, Mosul,, Iraq
2 College of Science, University of Mosul, Mosul,, Iraq
3 College of Pharmacy, Ninevah University, Mosul,, Iraq
     

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Medical leeches are ectoparasites invertebrates which are widely used in medical and surgical treatments because it was found since the ancient time that many diseases can be cured by medicinal leeches, then it was discovered that leech saliva contains anti-inflammatory, analgesic, antibacterial, anticoagulant, and even anticancer enzymes, also they found that medicinal leeches have a great role in treating diabetic ulcers. Tow samples of leeches were collected from two regions in Iraq from September to the end of December 2020. These samples were examined a live by dissecting microscope and then they were examined after fixing them in formaldehyde 5%. The samples of leeches that were used to analyze their genetic DNA were fixed and preserved in absolute ethanol. The classification process was done through a molecular diagnosis of samples of medical leeches by using polymerase chain reaction PCR and technique tracking nitrogenous bases in the nucleic acid chain, and the molecular diagnosis depending on Cytochrome oxidase subunit 1 (CO1), and then the result of PCR of medical leech samples was sent with the primers of the resulting bands and the gene sequences were read depending on the Genetic Analyser Device 3130 supplied by Japanese Hitachi company. Then the gene sequences were matched with gene sequences documented in the National Center Biotechnology Information NCBI. The results were analyzed depending on the BLAST program through which two types of medical leeches were detected Hirudo medicinalis and Hirudo verbena.

Keywords

Leeches, Hirudo medicinalis, Hirudo verbena, PCR.
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  • Trontelj P. Sotler M. Verovnik R. Genetic Differentiation Between Two Species of the Medicinal Leech, Hirudo Medicinalis and the Neglected H. Verbana, Based on Random-Amplified Polymorphic DNA. Parasitology Research. 2004; 94(2):118-24.doi.org/10.1007/s00436-004-1181-x.

  • Darabi-Darestani K. Malek M. Seasonal Variation in the Occurrence of the Medicinal Leech Hirudo Orientalis in Guilan Province, Iran. Aquatic Biology. 2011;11(3):289-94. doi.org/10.3354/ab00310.

  • Kutschera U. Shain DS. Hirudinea Lamarck 1818: Evolutionary Origin and Taxonomy of the Six Medicinal Leeches (Genus Hirudo) Known Today. Biomed Res. 2019;3:1-4. doi.10.15761/BRR.1000126.

  • Babenko VV. Podgorny OV. Manuvera VA. Kasianov AS. Manolov AI. Grafskaia EN et al. Draft Genome Sequences of Hirudo Medicinalis and Salivary Transcriptome of Three Closely Related Medicinal Leeches. BMC Genomics. 2020;21:1-6. doi.org/10.1186/s12864-020-6748-0.

  • Trontelj P. Sket B. Molecular Re-assessment of Some Phylogenetic, Taxonomic and Biogeographic Relationships Between the Leech Genera Dina and Trocheta (Hirudinea: Erpobdellidae). Hydrobiologia. 2000;438(1):227-35. doi.org/10.1023/A:1004137300113.

  • Govedich FR. Bain BA. Burd M. Davies RW. Reproductive Biology of the Invasive Asian Freshwater Leech Barbronia Weberi (Blanchard, 1897). Hydrobiologia. 2003; 510(1):125-9. doi.org/10.1023/B:HYDR. 0000008638.87536.b0.

  • Gouck HK. Protection from Ticks, Fleas, Chiggers, and Leeches. Archives of dermatology. 1966; 93(1):112-3. doi:10.1001/archderm.1966.01600190118026.

  • Kadry AA, El-Ganiny AM, El-Baz AM. Comparison of methods used in identification of Candida albicans. Research Journal of Pharmacy and Technology. 2018;11(3):1164-8. doi.org.10.5958/0974-360X.2018.00217.2

  • Jasim HS. Molecular detection and DNA Methylation of HBV in a group of Iraqi patients with Hepatocellular Carcinoma. Liver. 2019 Sep 30;20:100.

  • Rupashri SV, Gopinath P. Detection of CNA Gene for the presence of collagen adhesion in clinical strains of Staphylococcus aureus. Research Journal of Pharmacy and Technology. 2016 Oct 28;9(10):1626-8. doi.org.10.5958/0974-360X.2016.00324.3

  • Schmidt-Rhaesa A. Harzsch S. Purschke G. Structure and Evolution of Invertebrate Nervous Systems. Oxford University Press; 2015. DOI:10.1093/acprof:oso/9780199682201.001.0001.

  • Koperski P. Testing the Suitability of Leeches (Hirudinea, Clitellata) for Biological Assessment of Lowland Streams. Polish Journal of Ecology. 2005;53(1):65-80.

  • Todorov M. Grozeva S. Hubenov Z. Kenderov L. Trichkova T. Taxonomic Status and Distribution of Medicinal Leeches of the Genus Hirudo L. (Hirudinea) in Bulgaria. Acta zool. bulg. 2016; 68(2):171-82.

  • Elliott JM. Kutschera U. Medicinal Leeches: Historical Use, Ecology, Genetics and Conservation. Freshwater Reviews. 2011; 4(1):21-41. doi.org/10.1608/FRJ-4.1.417.

  • Elliott JM. Dobson M. Freshwater Leeches (Hirudinea) of Britain and Ireland; Keys and a Review of Their Ecology. Freshwater Biological Association, Scientific Publication. 2014;69.

  • Agnarsson I. Kuntner M. Taxonomy in a Changing World: Seeking Solutions for a Science in Crisis. Systematic Biology. 2007; 56(3):531-9. doi.org/10.1080/10635150701424546.

  • Siddall ME. Burreson EM. Phylogeny of Leeches (Hirudinea) Based on Mitochondrial Cytochrome Oxidase Subunit I. Molecular Phylogenetics and Evolution. 1998;9(1):156-62. doi.org/10.1006/mpev. 1997.0455.


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  • A Taxonomic Study Comparing the Two Types of Medicinal Leeches Available in Iraq

Abstract Views: 155  |  PDF Views: 0

Authors

Islam Khalid
College of Pharmacy, University of Mosul, Mosul,, Iraq
Najah Sobhi Nayyef
College of Science, University of Mosul, Mosul,, Iraq
Marwan M. Merkhan
College of Pharmacy, Ninevah University, Mosul,, Iraq

Abstract


Medical leeches are ectoparasites invertebrates which are widely used in medical and surgical treatments because it was found since the ancient time that many diseases can be cured by medicinal leeches, then it was discovered that leech saliva contains anti-inflammatory, analgesic, antibacterial, anticoagulant, and even anticancer enzymes, also they found that medicinal leeches have a great role in treating diabetic ulcers. Tow samples of leeches were collected from two regions in Iraq from September to the end of December 2020. These samples were examined a live by dissecting microscope and then they were examined after fixing them in formaldehyde 5%. The samples of leeches that were used to analyze their genetic DNA were fixed and preserved in absolute ethanol. The classification process was done through a molecular diagnosis of samples of medical leeches by using polymerase chain reaction PCR and technique tracking nitrogenous bases in the nucleic acid chain, and the molecular diagnosis depending on Cytochrome oxidase subunit 1 (CO1), and then the result of PCR of medical leech samples was sent with the primers of the resulting bands and the gene sequences were read depending on the Genetic Analyser Device 3130 supplied by Japanese Hitachi company. Then the gene sequences were matched with gene sequences documented in the National Center Biotechnology Information NCBI. The results were analyzed depending on the BLAST program through which two types of medical leeches were detected Hirudo medicinalis and Hirudo verbena.

Keywords


Leeches, Hirudo medicinalis, Hirudo verbena, PCR.

References