Research Journal of Pharmacy and Technology

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A Comparative Account on Antioxidant Activities, Total Phenolic and Flavonoid Contents of Punica Granatum, Carica Papaya, Foeniculum Vulgare, Trigonella Foenum-graecum, and Urtica Dioica: An in Vitro Evaluation


Affiliations
1 School of Bio-Engineering and Bio-Sciences, Lovely Professional University Jalandhar Delhi GT Road, Phagwara-144411, India
     

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Antioxidant potential of aqueous extracts was determined individually and ABTS/DPPH free radicals were utilized for scoring antioxidant activities, Folin-Ciocalteau’s reagent for Total Phenolic content and aluminum chloride colorimetry method for Total Flavonoid content evaluation. Different parts of the same plant exhibited different antioxidant potential. An overall comparative account among the different parts of selected fruits, spices and herb can be stated as Punica granatum (PG) peel˃ Punica granatum pulp˃ Carica papaya (CP) peel˃ Trigonella foenum-graecum (TFG) seeds˃ Foeniculum vulgare (FV) seeds˃ Urtica dioica (UD) dried leaves˃ Carica papaya (CP) seed˃ Urtica dioica (UD) frozen leaves˃ Punica granatum (PG) seed˃ Urtica dioica (UD) fresh leaves˃ Carica papaya (CP) pulp. Thus we can conclude that different parts of the same plant can have statistically different antioxidant potential and non-conventional parts can have good antioxidant potential which can be utilized to decrease the free radicals.

Keywords

Nutraceuticals, Antioxidant activity, ABTS/DPPH, Total phenolic content, Total flavonoid content.
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  • Suresh AS, Pawar B. A Study of Factors of Lifestyle and its impact on Nutraceutical Consumption: India Perspective. Asian Journal of Management. 2018; 9(1):203-11.

  • Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry. 2001; 49(11): 5165-5170.

  • Sharma S, Rana M, Kumar H, Parashar B. It's era to move towards nature for getting beneficial effects of plants having Antioxidant activity to fight against deleterious diseases. Asian Journal of Pharmaceutical Research. 2013; 3(2):103-6.

  • Shinde N, Bangar B, Deshmukh S, Kumbhar P. Nutraceuticals: A Review on current status. Research J. Pharm. and Tech. 2014; 7(1): 110-113.

  • G.Sreeramya, C.N.Nalini, Ramalakshmi. N, Kondaviti Sahini, Amudha Lakshmi.S. A new Era in Medicine – Neutraceuticals. Research J. Pharm. and Tech. 2018; 11(8): 3572-3576.

  • Jadhav GB, Saudagar RB. Free radical Scavenging and Antioxidant Activity of Punica granatum Linn. Asian Journal of Research in Pharmaceutical Science. 2014; 4(2): 51-4.

  • Tehranifar A, Zarei M, Nemati Z, Esfandiyari B, Vazifeshenas MR. Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars. Scientia Horticulturae. 2010; 126(2): 180-185.

  • Vij T, Prashar Y. A review on medicinal properties of Carica papaya Linn. Asian Pacific Journal of Tropical Disease. 2015; 5(1): 1-6.

  • Kanagavalli M., Anuradha R. A Study on Phytochemical Constituents and In Vitro Antioxidant Activity of Carica papaya. Research J. Pharm. and Tech. 2012; 5(1): 119-120.

  • Atta-Aly MA. Fennel swollen base yield and quality as affected by variety and source of nitrogen fertilizer. Scientia Horticulturae. 2001; 88(3):191-202.

  • Belguith-Hadriche O, Bouaziz M, Jamoussi K, Simmonds MS, El Feki A, Makni-Ayedi F. Comparative study on hypocholesterolemic and antioxidant activities of various extracts of fenugreek seeds. Food Chemistry. 2013; 138(2-3): 1448-1453.

  • Riehemann K, Behnke B, Schulze-Osthoff K. Plant extracts from stinging nettle (Urtica dioica), an antirheumatic remedy, inhibit the proinflammatory transcription factor NF-κB. FEBS Letters. 1999; 442(1):89-94.

  • Madhu C, Swapna J, Neelima K, Shah MV. A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in India. Asian Journal of Research in Pharmaceutical Science. 2012;2(3):98-100.

  • Neelam Jain, Shaily Goyal, K. G. Ramawat. Radical Scavenging Activity and Total Phenolic Content in Selected Fruits and Vegetables. Research J. Pharm. and Tech. 2012; 5(1): Page 121-124.

  • Lokhande SS. Role of Nutraceuticals in Various Diseases: A Comprehensive Review. Asian Journal of Pharmaceutical Research. 2018; 8(4): 236-40.

  • Seema Thakur, Neha Srivastava. Nutraceuticals: A Review. Asian J. Res. Pharm. Sci. 2016; 6(2): 85-94.

  • Vipul Singh, Sangeeta Mahaur, Sanjay Kumar Kushwaha. Nutraceuticals: A New Golden Era in Health and Disease. Asian J. Research Chem. 2018; 11(3): 652-658.

  • Rajasekaran A, Sivagnanam G, Xavier R. Nutraceuticals as therapeutic agents: A Review. Research J. Pharm. and Tech. 2008; 1(4): 171-174.

  • Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, Leitao SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 2001; 15(2): 127-130.

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999; 26(9-10): 1231-1237.

  • Malik CP and Singh MB. Plant enzymology and histo-enzymology, New Delhi.1980.

  • Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64(4): 555-559.

  • Elfalleh W, Nasri N, Marzougui N, Thabti I, M'rabet A, Yahya Y, Lachiheb B, Guasmi F, Ferchichi A. Physico-chemical properties and DPPH-ABTS scavenging activity of some local pomegranate (Punica granatum) ecotypes. International Journal of Food Sciences and Nutrition. 2009; 60(sup2): 197-210.

  • Elfalleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A. Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. Journal of Medicinal Plants Research. 2012; 6(32): 4724-4730.

  • Mansour E, Khaled AB, Lachiheb B, Abid M, Bachar K, Ferchichi A. Phenolic Compounds, Antioxidant, and Antibacterial Activities of Peel Extract from Tunisian Pomegranate. 2018.

  • Ardekani MR, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, Gholam N, Moridi T. Comparative antioxidant activity and total flavonoid content of Persian pomegranate (Punica granatum L.) cultivars. Iranian Journal of Pharmaceutical Research. 2011; 10(3): 519.

  • Jing PU, Ye T, Shi H, Sheng Y, Slavin M, Gao B, Liu L, Yu LL. Antioxidant properties and phytochemical composition of China-grown pomegranate seeds. Food Chemistry. 2012; 132(3): 1457-1464.

  • Ambigaipalan P, de Camargo AC, Shahidi F. Phenolic compounds of pomegranate byproducts (outer skin, mesocarp, divider membrane) and their antioxidant activities. Journal of Agricultural and Food Chemistry. 2016; 64(34): 6584-6604.

  • Morais DR, Rotta EM, Sargi SC, Schmidt EM, Bonafe EG, Eberlin MN, Sawaya AC, Visentainer JV. Antioxidant activity, phenolics and UPLC–ESI (–)–MS of extracts from different tropical fruits parts and processed peels. Food Research International. 2015; 77: 392-399.

  • Somanah J, Bourdon E, Rondeau P, Bahorun T, Aruoma OI. Relationship between fermented papaya preparation supplementation, erythrocyte integrity and antioxidant status in pre-diabetics. Food and Chemical Toxicology. 2014; 65: 12-17.

  • Rivera-Pastrana DM, Gardea AA, Yahia EM, Martínez-Téllez MA, González-Aguilar GA. Effect of UV-C irradiation and low temperature storage on bioactive compounds, antioxidant enzymes and radical scavenging activity of papaya fruit. Journal of Food Science and Technology. 2014; 51(12): 3821-3829.

  • Gogna N, Hamid N, Dorai K. Metabolomic profiling of the phytomedicinal constituents of Carica papaya L. leaves and seeds by 1H NMR spectroscopy and multivariate statistical analysis. Journal of Pharmaceutical and Biomedical Analysis. 2015; 115: 74-85.

  • Zhou K, Wang H, Mei W, Li X, Luo Y, Dai H. Antioxidant activity of papaya seed extracts. Molecules. 2011; 16(8): 6179-6192.

  • Siddique S, Nawaz S, Muhammad F, Akhtar B, Aslam B. Phytochemical screening and in-vitro evaluation of pharmacological activities of peels of Musa sapientum and Carica papaya fruit. Natural Product Research. 2018; 32(11): 1333-1336.

  • Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Research International. 2011; 44(7): 2047-2053.

  • Gomes WF, França FR, Denadai M, Andrade JK, da Silva Oliveira EM, de Brito ES, Rodrigues S, Narain N. Effect of freeze-and spray-drying on physico-chemical characteristics, phenolic compounds and antioxidant activity of papaya pulp. Journal of Food Science and Technology. 2018; 55(6): 2095-2102.

  • Sofi FR, Raju CV, Lakshmisha IP, Singh RR. Antioxidant and antimicrobial properties of grape and papaya seed extracts and their application on the preservation of Indian mackerel (Rastrelliger kanagurta) during ice storage. Journal of Food Science and Technology. 2016; 53(1): 104-117.

  • Baba WN, Tabasum Q, Muzzaffar S, Masoodi FA, Wani I, Ganie SA, Bhat MM. Some nutraceutical properties of fenugreek seeds and shoots (Trigonella foenum-graecum L.) from the high Himalayan region. Food Bioscience. 2018; 23: 31-37.

  • Anwar F, Ali M, Hussain AI, Shahid M. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour and Fragrance Journal. 2009; 24(4) :170-176.

  • Kim IS, Yang MR, Lee OH, Kang SN. Antioxidant activities of hot water extracts from various spices. International Journal of Molecular Sciences. 2011; 12(6): 4120-4131.

  • Hou WC, Lin RD, Cheng KT, Hung YT, Cho CH, Chen CH, Hwang SY, Lee MH. Free radical-scavenging activity of Taiwanese native plants. Phytomedicine. 2003; 10(2-3): 170-175.

  • Kenny O, Smyth TJ, Hewage CM, Brunton NP. Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of fenugreek (Trigonella foenum-graecum) seeds and bitter melon (Momordica charantia) fruit. Food Chemistry. 2013; 141(4): 4295-4302.

  • Schlesier K, Harwat M, Böhm V, Bitsch R. Assessment of antioxidant activity by using different in vitro methods. Free Radical Research. 2002; 36(2): 177-187.

  • Carvalho AR, Costa G, Figueirinha A, Liberal J, Prior JA, Lopes MC, Cruz MT, Batista MT. Urtica spp.: Phenolic composition, safety, antioxidant and anti-inflammatory activities. Food Research International. 2017; 99: 485-494.

  • Kukric ZZ, Topalic-Trivunovic LN, Kukavica BM, Matos SB, Pavicic SS, Boroja MM, Savic AV. Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.). Acta Periodica Technologica. 2012; 43: 257-272.

  • Fattahi S, Zabihi E, Abedian Z, Pourbagher R, Ardekani AM, Mostafazadeh A, Akhavan-Niaki H. Total phenolic and flavonoid contents of aqueous extract of stinging nettle and in vitro antiproliferative effect on hela and BT-474 Cell lines. International Journal of Molecular and Cellular Medicine. 2014; 3(2): 102.

  • Himalian R, Singh SK, Singh MP Ameliorative role of nutraceuticals on neurodegenerative diseases using the Drosophila melanogaster as a discovery model to define bioefficacy, Journal of the American College of Nutrition. 2021: DOI: 10.1080/07315724.2021.1904305


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  • A Comparative Account on Antioxidant Activities, Total Phenolic and Flavonoid Contents of Punica Granatum, Carica Papaya, Foeniculum Vulgare, Trigonella Foenum-graecum, and Urtica Dioica: An in Vitro Evaluation

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Authors

Ranjana Himalian
School of Bio-Engineering and Bio-Sciences, Lovely Professional University Jalandhar Delhi GT Road, Phagwara-144411, India
Mahendra P. Singh
School of Bio-Engineering and Bio-Sciences, Lovely Professional University Jalandhar Delhi GT Road, Phagwara-144411, India

Abstract


Antioxidant potential of aqueous extracts was determined individually and ABTS/DPPH free radicals were utilized for scoring antioxidant activities, Folin-Ciocalteau’s reagent for Total Phenolic content and aluminum chloride colorimetry method for Total Flavonoid content evaluation. Different parts of the same plant exhibited different antioxidant potential. An overall comparative account among the different parts of selected fruits, spices and herb can be stated as Punica granatum (PG) peel˃ Punica granatum pulp˃ Carica papaya (CP) peel˃ Trigonella foenum-graecum (TFG) seeds˃ Foeniculum vulgare (FV) seeds˃ Urtica dioica (UD) dried leaves˃ Carica papaya (CP) seed˃ Urtica dioica (UD) frozen leaves˃ Punica granatum (PG) seed˃ Urtica dioica (UD) fresh leaves˃ Carica papaya (CP) pulp. Thus we can conclude that different parts of the same plant can have statistically different antioxidant potential and non-conventional parts can have good antioxidant potential which can be utilized to decrease the free radicals.

Keywords


Nutraceuticals, Antioxidant activity, ABTS/DPPH, Total phenolic content, Total flavonoid content.

References