Research Journal of Pharmacy and Technology

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Evaluation of Safety and Efficacy of an Ayurvedic Ointment Against Acute Burn Injury in Wistar Rats


Affiliations
1 Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
2 Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Kothrud, Pune, Maharashtra 411038., India
     

   Subscribe/Renew Journal


Skin burn is a common health problem that needs a fast and accurate treatment; else, it can have a worse prognosis. The available treatment options pose limitations due to their adverse effects; thus there is an urgent need to find newer treatment options for burn wound healing that can provide a safer and faster treatment option. Ayurveda gives a plethora of medicinal plants and their formulations which have been used in the management of burn wound. The Ayurvedic ointment formulated in this experiment utilizes essential ayurvedic ingredients which are known for their burn wound healing potential. The current work includes the formulation of the Ayurvedic ointment, its standardization, toxicity and efficacy study. The Ayurvedic ointment was formulated by fusion method, and its physicochemical characterization ensures the purity of the compounds used for the formulation. The ointment formulated is well set under the safety parameters for the presence of heavy metals and also free from microbial and fungal contamination. It has obtained optimum spreadability and viscosity, making it desirable for application on wounds. In the acute dermal toxicity study, it has shown no adverse effect on the animals as there were no significant changes in the body weight and feed intake and water consumption, thus confirming it as a safe formulation. It has shown significant rate of wound healing and period of re-epithelization in the Wistar rat model. It can be a potential ointment for burn wound treatment, and in future, it can be considered for clinical studies to validate its safety and efficacy in humans.

Keywords

Burn wound, Ayurveda, Ointment, Fusion method, Re-epithelization.
Subscription Login to verify subscription
User
Notifications
Font Size


  • Weaver Jr AJ, Brandenburg KS, Smith BW, Leung KP. Comparative analysis of the host response in a rat model of deep-partial and full-thickness burn wounds with Pseudomonas aeruginosa Infection. Frontiers in Cellular and Infection Microbiology. 2020; 9:466.

  • Amini-Nik S, Yousuf Y, Jeschke MG. Scar management in burn injuries using drug delivery and molecular signaling: current treatments and future directions. Advanced Drug Delivery Reviews. 2018; 123:135-154.

  • Kaddoura I, Abu-Sittah G, Ibrahim A, Karamanoukian R, Papazian N. Burn injury: review of pathophysiology and therapeutic modalities in major burns. Annals of burns and Fire Disasters. 2017; 30 (2):95-102.

  • Vaghardoost R, Majd SGM, Tebyanian H, Babavalian H, Malaei L, Niazi M, Javdani A. The healing effect of sesame oil, camphor and honey on second degree burn wounds in rat. World Journal of Plastic Surgery. 2018; 7(1):67-71.

  • Quan TE, Cowper S, Wu S-P, Bockenstedt LK, Bucala R. Circulating fibrocytes: collagen-secreting cells of the peripheral blood. The International Journal of iochemistry & Cell Biology. 2004; 36(4):598-606.

  • Abdullahi A, Amini-Nik S, Jeschke M. Animal models in burn research. Cellular and Molecular Life Sciences. 2014; 71 (17):3241-3255.

  • Chang S-J, Sartika D, Fan G-Y, Cherng J-H, Wang Y-W. (2019) Animal models of burn wound management. in: Animal Models in Medicine and Biology. 2019. https://www.intechopen.com/chapters/69011.

  • Nasiri E, Hosseinimehr SJ, Azadbakht M, Akbari J, Enayati-Fard R, Azizi S, Azadbakht M. The healing effect of Arnebia euchroma ointment versus silver sulfadiazine on burn wounds in rat. World Journal of Plastic surgery. 2015; 4(2):134-144.

  • Hosseini SV, Tanideh N, Kohanteb J, Ghodrati Z, Mehrabani D, Yarmohammadi H. Comparison between Alpha and silver sulfadiazine ointments in treatment of Pseudomonas infections in 3rd degree burns. International Journal of Surgery. 2007; 5(1):23-26.

  • Bahramsoltani R, Farzaei MH, Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Archives of Dermatological Research. 2014; 306 (7):601-617.

  • Silalahi J, Surbakti C. Burn wound healing activity of hydrolyzed virgin coconut oil. International Journal of PharmaTech Research. 2015; 8 (1):67-73.

  • Molee W, Phanumartwiwath A, Kesornpun C, Sureram S, Ngamrojanavanich N, Ingkaninan K, Mahidol C, Ruchirawat S, Kittakoop P. Naphthalene derivatives and quinones from Ventilago denticulata and their nitric oxide radical scavenging, antioxidant, cytotoxic, antibacterial, and phosphodiesterase inhibitory activities. Chemistry & biodiversity, 2018; 15 (3):e1700537. doi: 10.1002/cbdv.201700537.

  • Kulac M, Aktas C, Tulubas F, Uygur R, Kanter M, Erboga M, Ceber M, Topcu B, Ozen OA. The effects of topical treatment with curcumin on burn wound healing in rats. Journal of molecular histology. 2013; 44 (1):83-90.

  • Khan M, Shahzadi T, Malik S, Shahid M, Ismail M, Zubair M, Iqbal S. Pharmacognostic evaluation of turmeric (Curcuma longa) extracts in diabetic wound healing. The Journal of Animal & Plant Sciences. 2019; 29 (1):68-74.

  • Mahboubi M. Rosa damascena as holy ancient herb with novel applications. Journal of Traditional and Complementary Medicine. 2016; 6 (1):10-16.

  • Busse D, Kudella P, Grüning N-M, Gisselmann G, Ständer S, Luger T, Jacobsen F, Steinsträßer L, Paus R, Gkogkolou P. A synthetic sandalwood odorant induces wound-healing processes in human keratinocytes via the olfactory receptor OR2AT4. Journal of investigative dermatology. 2014; 134 (11): 2823-2832.

  • Palatty P, Azmidah A, Rao S, Jayachander D, Thilakchand K, Rai M, Haniadka R, Simon P, Ravi R, Jimmy R. Topical application of a sandal wood oil and turmeric based cream prevents radiodermatitis in head and neck cancer patients undergoing external beam radiotherapy: a pilot study. The British Journal of Radiology. 2014; 87 (1038): 20130490.

  • Temhunna S, Mishra S, Parida N. An Ayurvedic vivechan on arsha vyadhi. International Journal of Health Sciences and Research. 2019; 9 (7):277-281.

  • Khan J, Leenoos LM, Ruhi S, Al-Dhalli S, Kaleemullah M, Saad R, Ali HS, Sahu R, Florence M, Rasny M. Development and evaluation of polyherbal halal ointment using honey and Papaya. International Journal of Medical Toxicology & Legal Medicine. 2020; 23 (1-2): 232-238.

  • Bora M, Srivastava B, Gaidhani SN, Sharma H, Gautam MK, Tiwari RK, Wanjari MM, Khanduri S, Hazra J. Development of a novel polyherbal formulation for augmenting milk production in healthy dairy cows. Journal of Drug Research in Ayurvedic Sciences. 2019; 4(2): 84-94.

  • Gupta S, Raghuwanshi N, Varshney R, Banat IM, Srivastava AK, Pruthi PA, Pruthi V. Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute. Biomedicine & Pharmacotherapy. 2017; 94:1186-1196.

  • Bhatia N, Arunpreet S, Rohit S, Amandeep S, Varinder S, Gurjeet S, Jaideep B, Ravi D, Balwinder S. Evaluation of burn wound healing potential of aqueous extract of Morus alba based cream in rats. Journal of Phytopharmacology. 2014; 3 (6):378-383.

  • Liu Y, Xiao X, Ji L, Xie L, Wu S, Liu Z. Camellia cake extracts reduce burn injury through suppressing inflammatory responses and enhancing collagen synthesis. Food & Nutrition Research. 2020; 64: 10.29219/fnr.v64.3782.

  • Banchrof J, Steven A, Turner D. Theory and practice of histopathological techniques. Churchil Livingstone, New York, London, San Francisco, Tokyo. 1996.

  • Kumar KNS, Holla BS, Ravishankar B, Yashovarma B. Quality standards for Hutabhugadi curṇa (Ayurvedic Formulary of India). Journal of traditional and complementary medicine. 2016, 6 (1):78-88.

  • Abraham A, Samuel S, Mathew L. (2018) Phytochemical analysis of Pathyashadangam kwath and its standardization by HPLC and HPTLC. Journal of Ayurveda and Integrative Medicine. 2020; 11(2): 153-158.

  • Pahl P. Growth curves for body weight of the laboratory rat. Australian journal of biological sciences. 1969; 22 (4):1077-1080.

  • Shahzad MN, Ahmed N. Effectiveness of Aloe vera gel compared with 1% silver sulphadiazine cream as burn wound dressing in second degree burns. Journal of Pakistan Medical Association. 2013; 63 (2):225-230.

  • Alemdaroglu C, Degim Z, Çelebi N, Zor F, Ozturk S, Erdogan D. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns. 2006; 32 (3): 319-327.


Abstract Views: 160

PDF Views: 0




  • Evaluation of Safety and Efficacy of an Ayurvedic Ointment Against Acute Burn Injury in Wistar Rats

Abstract Views: 160  |  PDF Views: 0

Authors

Dipsundar Sahu
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
Manajit Bora
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
A. K. Dixit
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
Shrirang Jamadagni
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Kothrud, Pune, Maharashtra 411038., India
Manosi Das
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
Barnail Sinha
, India
Qadir Alam
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
Sharad Pawar
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India
P. V.Vara Prasad
Central Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Govt. of India, Bidhannagar, Kolkata, West Bengal 700091., India

Abstract


Skin burn is a common health problem that needs a fast and accurate treatment; else, it can have a worse prognosis. The available treatment options pose limitations due to their adverse effects; thus there is an urgent need to find newer treatment options for burn wound healing that can provide a safer and faster treatment option. Ayurveda gives a plethora of medicinal plants and their formulations which have been used in the management of burn wound. The Ayurvedic ointment formulated in this experiment utilizes essential ayurvedic ingredients which are known for their burn wound healing potential. The current work includes the formulation of the Ayurvedic ointment, its standardization, toxicity and efficacy study. The Ayurvedic ointment was formulated by fusion method, and its physicochemical characterization ensures the purity of the compounds used for the formulation. The ointment formulated is well set under the safety parameters for the presence of heavy metals and also free from microbial and fungal contamination. It has obtained optimum spreadability and viscosity, making it desirable for application on wounds. In the acute dermal toxicity study, it has shown no adverse effect on the animals as there were no significant changes in the body weight and feed intake and water consumption, thus confirming it as a safe formulation. It has shown significant rate of wound healing and period of re-epithelization in the Wistar rat model. It can be a potential ointment for burn wound treatment, and in future, it can be considered for clinical studies to validate its safety and efficacy in humans.

Keywords


Burn wound, Ayurveda, Ointment, Fusion method, Re-epithelization.

References