Research Journal of Pharmacy and Technology

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Isolation and Identification of Antibacterial Compound from Actinomycetes Isolated from Mangrove Soil


Affiliations
1 Department of Biotechnology, MITS School of Biotechnology, Odisha - 751024, India
2 S.K. Dash Centre of Excellence of Biosciences and Engineering and Technology, Indian Institute of Technology, Bhubaneswar - 752050, India
3 Department of Biotechnology, School of Health and Allied Sciences, ARKA JAIN University, Jamshedpur – 831001, India
4 School of Chemical Sciences, National Institute of Science Education and Research, Bhubaneswar - 752050, India
5 District Public Health Laboratory, District Head Quarters Hospital, Baripada - 757001, India
6 Department of Biotechnology and Bioinformatics, Sambalpur University Jyotivihar - 768019, India
     

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Nine actinobacteria were isolated from the mangrove soil of Bhitarkanika and screened for their bioactive metabolites and antimicrobial property. Out of the nine actinobacterial isolates, the bacterial isolateBAB-7 showed positive activity towards most of the enzymesscreened,i.e., Cellulase, phosphate solubilization, L-asparaginase, protease, and amylase. The actinobacterial isolate BAB-7 also exhibited antimicrobial activity against K. pneumoniae, V. alginolyticus, V. parahaemolyticus, P. aeruginosa,E. coli, S. typhimurium, and S. salivarius. Based on biochemical characterization, the actinobacterial isolate BAB-7 was identified asSteptomcessp.The secondary metabolites produced by the bacterial strain were analyzed through GC-mass spectroscopy and found to besimilar to the peaks of2-cyclohex-3-en-1-yl-2-oxoacetic acid derivative (ketomycin).

Keywords

Antibiotics, bioactive metabolites, actinobacteria, mangrove.
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  • Isolation and Identification of Antibacterial Compound from Actinomycetes Isolated from Mangrove Soil

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Authors

Riya Jalan
Department of Biotechnology, MITS School of Biotechnology, Odisha - 751024, India
Biswaranjan Pradhan
S.K. Dash Centre of Excellence of Biosciences and Engineering and Technology, Indian Institute of Technology, Bhubaneswar - 752050, India
Santosh Kumar Singh
Department of Biotechnology, School of Health and Allied Sciences, ARKA JAIN University, Jamshedpur – 831001, India
Anuradha Das
School of Chemical Sciences, National Institute of Science Education and Research, Bhubaneswar - 752050, India
Rajib Lochan Barik
District Public Health Laboratory, District Head Quarters Hospital, Baripada - 757001, India
Jyotisikha Meher
Department of Biotechnology, MITS School of Biotechnology, Odisha - 751024, India
Rashmi Ranjan Mishra
Department of Biotechnology, MITS School of Biotechnology, Odisha - 751024, India
Debasmita Dubey
Department of Biotechnology and Bioinformatics, Sambalpur University Jyotivihar - 768019, India
Bikash Chandra Behera
Department of Biotechnology, MITS School of Biotechnology, Odisha - 751024, India

Abstract


Nine actinobacteria were isolated from the mangrove soil of Bhitarkanika and screened for their bioactive metabolites and antimicrobial property. Out of the nine actinobacterial isolates, the bacterial isolateBAB-7 showed positive activity towards most of the enzymesscreened,i.e., Cellulase, phosphate solubilization, L-asparaginase, protease, and amylase. The actinobacterial isolate BAB-7 also exhibited antimicrobial activity against K. pneumoniae, V. alginolyticus, V. parahaemolyticus, P. aeruginosa,E. coli, S. typhimurium, and S. salivarius. Based on biochemical characterization, the actinobacterial isolate BAB-7 was identified asSteptomcessp.The secondary metabolites produced by the bacterial strain were analyzed through GC-mass spectroscopy and found to besimilar to the peaks of2-cyclohex-3-en-1-yl-2-oxoacetic acid derivative (ketomycin).

Keywords


Antibiotics, bioactive metabolites, actinobacteria, mangrove.

References