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Characterization and Synthesis of Milk Thistle Nanoparticles to Overcome Oxidative Stress Induce Testicular Damage in Male Rats


Affiliations
1 University of Technology / Nanotechnology and Advanced Research Center., Iraq
2 College of Hilla University/ Department of Anesthetic., Iraq
3 Al-Safwa University College, Kerbala,, Iraq
4 Department of Pathology, Veterinary Medicine Collage, Karbala University., Iraq
5 College of Veterinary Medicine, AL-Qassim green University, Iraq

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Background Silymarin (SMN) is a natural component polyphenolic purified from Silybum marianum (milk thistle) seeds. SMN can control reactive oxygen species and converts them into compounds with minimizing their toxicity. Objectives The aim of the present study was to evaluate the therapeutic effect of silymarin nanoparticles on sperm parameters of rats induced diabetes by alloxan. Methods: Firstly gas liquid chromatography (GCMS) was utilized to identification active compound in milk thistle, then the preparation of silymarin -TPGS nanoparticles: Nanoparticles of silymarin of were prepared by the Nano precipitation. Forty Wistar albino rats were divided into four groups, control, induced diabetic with 150mg/kg of alloxan I.p, group three and four induced diabetic and treated with milk thistle extract and nanoparticles at a dose of 100 mg/kg.bw daily orally, for 60 days, Blood was collected from heart puncture for estimate serum FSH, L.H, and testosterone, then animal sacrificed to evaluate sperm quality and quantity as well as the histopathological section of tests. Results: Biochemical inspection appear that SMN nanoparticles improved sperm quality and quantity when compared with untreated and SMN groups. SMN nanoparticles appear to have therapeutic effects as an antioxidant against alloxan-induced damage in rat testes. Conclusions: Silymarin nanoparticles can be considered as a hopeful herbal as complementary medicine which may play a significant role to save normal spermatocytes against ROS effects-induced reproductive damage.

Keywords

Silymarin, FSH, L.H, Tests, Nanoparticles.
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Abstract Views: 253




  • Characterization and Synthesis of Milk Thistle Nanoparticles to Overcome Oxidative Stress Induce Testicular Damage in Male Rats

Abstract Views: 253  | 

Authors

Essa Daham. Jalloob
University of Technology / Nanotechnology and Advanced Research Center., Iraq
Rusul Heider
College of Hilla University/ Department of Anesthetic., Iraq
Abdulamir A. Al Zahid
Al-Safwa University College, Kerbala,, Iraq
Iman Jawad. Jaber
Department of Pathology, Veterinary Medicine Collage, Karbala University., Iraq
Adnan Mansour. Jasim
College of Veterinary Medicine, AL-Qassim green University, Iraq
Mohsin
College of Veterinary Medicine, AL-Qassim green University, Iraq
Kareem
College of Veterinary Medicine, AL-Qassim green University, Iraq
Rawaa
College of Veterinary Medicine, AL-Qassim green University, Iraq
Ameer Ridha. Dirwal
College of Veterinary Medicine, AL-Qassim green University, Iraq
Dheyaa Ali.Neamah
College of Veterinary Medicine, AL-Qassim green University, Iraq

Abstract


Background Silymarin (SMN) is a natural component polyphenolic purified from Silybum marianum (milk thistle) seeds. SMN can control reactive oxygen species and converts them into compounds with minimizing their toxicity. Objectives The aim of the present study was to evaluate the therapeutic effect of silymarin nanoparticles on sperm parameters of rats induced diabetes by alloxan. Methods: Firstly gas liquid chromatography (GCMS) was utilized to identification active compound in milk thistle, then the preparation of silymarin -TPGS nanoparticles: Nanoparticles of silymarin of were prepared by the Nano precipitation. Forty Wistar albino rats were divided into four groups, control, induced diabetic with 150mg/kg of alloxan I.p, group three and four induced diabetic and treated with milk thistle extract and nanoparticles at a dose of 100 mg/kg.bw daily orally, for 60 days, Blood was collected from heart puncture for estimate serum FSH, L.H, and testosterone, then animal sacrificed to evaluate sperm quality and quantity as well as the histopathological section of tests. Results: Biochemical inspection appear that SMN nanoparticles improved sperm quality and quantity when compared with untreated and SMN groups. SMN nanoparticles appear to have therapeutic effects as an antioxidant against alloxan-induced damage in rat testes. Conclusions: Silymarin nanoparticles can be considered as a hopeful herbal as complementary medicine which may play a significant role to save normal spermatocytes against ROS effects-induced reproductive damage.

Keywords


Silymarin, FSH, L.H, Tests, Nanoparticles.

References