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Method Development and Validation of Simultaneous Determination of Assay of Quinapril and Tolcapone
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A simple and selective HPLC method is developed for the simultaneous determination of quinapril and tolcopone. The chromatographic separation was achieved on a inertsil column, C18(150 X 4.6 ID) 5μm with mobile phase consisting of a mixture of 40 volumes of mixed phosphate buffer and 60 volumes of acetonitrile were prepared with a detection of wavelength of 239nm. The method was validated for analytical parameters such as specificity, accuracy, precision, robustness and ruggedness as per ICH guidelines. The correlation coefficient for linear curve obtained between concentration vs. Area for standard preparations of Quinapril and Tolcapone is 0.995 and 0.999. The percentage mean recovery of Quinapril and Tolcapone is 101.02% and 99.55% respectively. The proposed method was found to be simple, accurate, precise, and robust and stability indicating HPLC method. Hence this method can be used for routine analysis.
Keywords
RP-HPLC, Quinapril, Tolcapone, Method development, Validation.
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- Wishart DS. Jewison T. Guo AC, Wilson M and Knox C. et al The Human Metabolome Database. Nucleic Acids Res 2013; 1: 801-7 doi: 10.1093/nar/gks1065
- Espinoza S. Managó F. Leo D. et al Role of catechol-O-methyltransferase (COMT)- dependent processes in Parkinson’s disease and L-DOPA treatment. CNS Neurol. Disord. Drug Targets 2012; 11(3): 251-63. doi: 10.2174/187152712800672436
- Goncalves D. Alves G.Soares-Da-Silva P. Falcao A et al Bioanalytical chromatographic methods for the determination of catechol-O-methyltransferase inhibitors in rodents and human samples: a review. Anal. Chim. Acta 2012; 710: 17-32. DOI: 10.1016/j.aca.2011.10.026
- Bugamelli F. Marcheselli C. Barba E. Raggi MA. Et al Determination of L-dopa, carbidopa, 3-O-methyldopa and entacapone in human plasma by HPLC-ED. J Pharm Biomed Anal 2011; 54(3): 562-67. doi: 10.1016/j.jpba.2010.09.042
- Wright JM. Musini VM. Gill R.et al First-line drugs for hypertension. Cochrane Database Syst Rev 2018; 4: CD001841. doi: 10.1002/14651858.CD001841.pub3
- Long CM, Tang K. Chokshi H. Fotaki N et al Surface dissolution UV imaging for investigation of dissolution of poorly soluble drugs and their amorphous formulation. AAPS Pharm Sci Tech 2019; 20(3): 113. doi: 10.1208/s12249-019-1317-z.
- FDA Approved Drug Products: Quinapril Oral Tablet, www.accessdata.fda.gov/drugsatfda_docs/label/2017/019885s043lbl.pdf
- Begum MH: Analytical method development and validation for the estimation of quinapril and tolcapone using RP-HPLC. Int J of Innovative Pharmaceutical Sciences and Research 2018; 6(01): 99-113. doi: 10.13040/IJPSR.0975-8232.12(6).3375-80
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