Estimation of Rutin in Tephrosia purpurea by HPTLC Method

Tephrosia purpurea Linn (Family-Fabeaceae) having anti-hepatotoxic activity, CNS depressant activity and it also show anti-allergic activity. The plant Tephrosia purpurea contains rutin, quercetin, rotenoids, rotenone, tephrosin and lupeol. The powdered aerial part of Tephrosia purpuria was extracted with 95% ethanol by hot continuous extraction method. The prepared extracts were subjected to HPTLC analysis. The marker compounds were estimated by using pre-coated HPTLC aluminium silica gel 60 F254 plates (MERCK) and compared with standard. The methanolic solution of standard rutin 5 μl (1 mg/ml) and Tephrosia purpuria extract 8 μl (10 mg/ml) were applied as 7mm band width using CAMAG Linomat IV applicator. The Mobile phase is ethyl acetate: formic acid: glacial acetic acid: water (100:11:11:26). The detection was carried out at 366 nm. The amount of rutin was estimated by the comparing the peak area of standard and the same was present in the extracts. The content of rutin in Tephrosia purpurea extracts was found to be 1.40% w/w. This estimation technique is very much useful for the estimation of quercetin and rutin present in the various medicinal plants.