Green Synthesis and In vitro Applications of Nano Hydroxyapatite for Orthopaedic and Dental Applications

Hydroxyapatite (HAp) is a known Biomaterial applied in Orthopaedic and Dental application as bone implants. Bone can easily be infected by microorganisms. Hydroxyapatite with antibacterial activity can be used for bone and dental treatment defects. In the current study, HAp nanoparticles are synthesized with and without Moringa oleifera leaf extract by wet chemical method assisted by Microwave irradiation. Calcium hydroxide and Orthophosphoric acid are used as calcium and phosphorus source respectively. The synthesized samples were characterized by X-ray Diffraction technique (XRD), Fourier Transform Infrared analysis (FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDAX). Further in vitro analysis was performed using gram positive bacteria Staphylococcus aureus, gram negative bacteria Klebsiella pneumonia and fungi Candida albicans. X-ray Diffraction (XRD) techniques investigated the crystalline size for both the samples ranges between 6 to 20 nm. FTIR spectrum confirms the presence of hydroxyl (O-H) and phosphate (PO₄ ^3-) groups. SEM and EDAX predict the presence of spherical shaped morphology with proper proportion of 1.67. In vitro analysis reveals the significant zone of inhibition for both bacteria and fungi.