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Green Synthesis and In vitro Applications of Nano Hydroxyapatite for Orthopaedic and Dental Applications


Affiliations
1 Department of Physics, Navarasam Arts and Science College for Women, Erode, India
2 Department of Physics, Sri Sarada College for Women, Salem, Tamil Nadu, India
 

Hydroxyapatite (HAp) is a known Biomaterial applied in Orthopaedic and Dental application as bone implants. Bone can easily be infected by microorganisms. Hydroxyapatite with antibacterial activity can be used for bone and dental treatment defects. In the current study, HAp nanoparticles are synthesized with and without Moringa oleifera leaf extract by wet chemical method assisted by Microwave irradiation. Calcium hydroxide and Orthophosphoric acid are used as calcium and phosphorus source respectively. The synthesized samples were characterized by X-ray Diffraction technique (XRD), Fourier Transform Infrared analysis (FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDAX). Further in vitro analysis was performed using gram positive bacteria Staphylococcus aureus, gram negative bacteria Klebsiella pneumonia and fungi Candida albicans. X-ray Diffraction (XRD) techniques investigated the crystalline size for both the samples ranges between 6 to 20 nm. FTIR spectrum confirms the presence of hydroxyl (O-H) and phosphate (PO4 3-) groups. SEM and EDAX predict the presence of spherical shaped morphology with proper proportion of 1.67. In vitro analysis reveals the significant zone of inhibition for both bacteria and fungi.


Keywords

HAp, Invitro Analysis, Synthesis.
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  • Iqbal N, et al. Characterization antibacterial and in vitro compatibility of zinc-silver doped hydroxyapatite nanoparticles prepared through microwave synthesis. Ceramic International. 2014; 40:4507–13. https://doi.org/10.1016/j.ceramint.2013.08.125

  • Kanchana P, et al. Influence of sodium fluoride on the synthesis of hydroxyapatite by gel method. Journal of Crystal Growth. 2010; 312:808–16. https://doi.org/10.1016/j.jcrysgro.2009.12.032

  • Suresh Kumar G, Rajendran S, Karthi S, et al. Green synthesis and antibacterial activity of hydroxyapatite nanorods for orthopedic applications. MRS Comm. 2017; 7:183–8.

  • Varun Prasath P, Ravichandran K, et al. Green synthesis of hydroxyapatite nano rods using Camellia sinesis (White tea extract). IJIRSE, 2347-3207

  • Sundrarajan M, Jegatheeswaran S, Selvam S, Sanjeevi N, Balaji M, et al. The ionic liquid assisted green synthesis of hydroxyapatite Nano plates by Moringa oleifera flower extract: A biomimetic approach. Materials and Design. 2015; 88:1183–90. https://doi.org/10.1016/j.matdes.2015.09.051

  • Kalaiselvi V, Mathammal R, Vijayakumar S, Vaseeharan B. Microwave assisted green synthesis of Hydroxyapatite nanorods using Moringa oleifera flower extract and its antimicrobial applications. IJVSM. 2018; 6(2):286–95. doi.org/10.1016/j.ijvsm.2018.08.003

  • Manju S, Malaikozhundan B, Vijayakumar S, Santhi S, Jaishabanu A, Ekambaram P, et al. Antibactrial, antibio film and cytoxic effects of Nigella sativa essential oil coated gold nanoparticles. Microb Pathog. 2016l 91:129–35. https://doi.org/10.1016/j.micpath.2015.11.021

  • Mariappan R, Ponnuswamy V, Suresh P. Effect of doping concentration on the structural and optical properties of pure and tin doped zinc oxide thin films by Nebulizer Spray Pyrolysis (NSP) technique. Superlattices Microstruct. 2012; 52:500–13. https://doi.org/10.1016/j.spmi.2012.05.016

  • Costescu A, Pasuk I, Ungureanu F, Dinischiotu A, Costache M, Huneau F, et al. Physico-chemical properties of Nanosized Hexagonal Hydroxyapatite powder synthesized by sol-gel. Dig J Nanomater Biostruct. 2010; 5:989–1000.

  • Thaya R, Malaikozhundan B, Vijayakumar S, Sivakamavalli J, Jeyasekar R, et al. Chitosan coated Ag/ZnO nanocomposite and their antibiofilm, antifungal and cytotoxic effects on murine macrophages. Microb pathog. 2016; 100:124–32. https://doi.org/10.1016/j.micpath.2016.09.010

  • Movasaghi Z, Rehman S, Rehman I, et al. Fourier transform infrared spectroscopy of biological tissues. Applied Spec Reviews. 2008; 43(2):134–79.

  • Archer NK, Mazaitis MJ, et al. Staphylococcus aureus biofilms. Properties, Regulation and Roles in Human Disease. 2011; 2(5):445–59.

  • Pandya HM, Anitha P, Mathammal R, Kalaiselvi V. Incorporation and in vitro application of hydroxyapatite with silver and titanium dopants synthesized by wet chemical method. J Environ Nanotechnol. 2017; 6:42–6. https://doi.org/10.13074/jent.2017.12.174290

  • Kalaiselvi V, Mathammal R. Influence and effects of pure and capped Hydroxyapatite Nanoparticles using HEK 293 cell line by Microwave irradiation method. Asian Journal of Chemistry. 2018; 38(8):1696–700. https://doi.org/10.14233/ajchem.2018.21214


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  • Green Synthesis and In vitro Applications of Nano Hydroxyapatite for Orthopaedic and Dental Applications

Abstract Views: 657  |  PDF Views: 252

Authors

V. Kalaiselvi
Department of Physics, Navarasam Arts and Science College for Women, Erode, India
K. Surya
Department of Physics, Navarasam Arts and Science College for Women, Erode, India
N. Vidhya
Department of Physics, Sri Sarada College for Women, Salem, Tamil Nadu, India
R. Mathammal
Department of Physics, Sri Sarada College for Women, Salem, Tamil Nadu, India

Abstract


Hydroxyapatite (HAp) is a known Biomaterial applied in Orthopaedic and Dental application as bone implants. Bone can easily be infected by microorganisms. Hydroxyapatite with antibacterial activity can be used for bone and dental treatment defects. In the current study, HAp nanoparticles are synthesized with and without Moringa oleifera leaf extract by wet chemical method assisted by Microwave irradiation. Calcium hydroxide and Orthophosphoric acid are used as calcium and phosphorus source respectively. The synthesized samples were characterized by X-ray Diffraction technique (XRD), Fourier Transform Infrared analysis (FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray analysis (EDAX). Further in vitro analysis was performed using gram positive bacteria Staphylococcus aureus, gram negative bacteria Klebsiella pneumonia and fungi Candida albicans. X-ray Diffraction (XRD) techniques investigated the crystalline size for both the samples ranges between 6 to 20 nm. FTIR spectrum confirms the presence of hydroxyl (O-H) and phosphate (PO4 3-) groups. SEM and EDAX predict the presence of spherical shaped morphology with proper proportion of 1.67. In vitro analysis reveals the significant zone of inhibition for both bacteria and fungi.


Keywords


HAp, Invitro Analysis, Synthesis.

References





DOI: https://doi.org/10.15613/sijrs%2F2017%2Fv4i2%2F184636