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In Vitro Propagation of a Sporadic Medicinal Plant Cleome monophylla L


Affiliations
1 PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
 

A sporadic medicinal plant Cleome monophylla L. comes under the family Capparidaceae selected for the in vitro regeneration. The leaf explants showed differential responses to various combination and concentrations of BAP, TDZ and NAA and produced yellow to green friable and compact and dense calli. The maximum amount of callus per explants was produced on MS medium with 4.44 µM of BAP, 1.16 µM of TDZ and 1.135 µM of NAA combination and concentration gave the 84% response and produced green compact calli, 19.98 ± 0.03 shoots from the callus and the maximum mean number of ischolar_mainlets per culture was also noted (15 ± 0.57) in this combination with MS medium of half strength. The in vitro developed entire plantlets were fruitfully acclimatized to thermocol cups and earthen pots.

Keywords

Callogenesis, Capparidaceae, Cleome monophylla, Growth Regulators, Shoot and Root Induction
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  • In Vitro Propagation of a Sporadic Medicinal Plant Cleome monophylla L

Abstract Views: 517  |  PDF Views: 285

Authors

Vijayashalini P.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
Abirami P.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India
Jayanthi G.
PG and Research, Department of Botany, Vellalar College for Women (Autonomous), Erode - 638 012, Tamil Nadu, India

Abstract


A sporadic medicinal plant Cleome monophylla L. comes under the family Capparidaceae selected for the in vitro regeneration. The leaf explants showed differential responses to various combination and concentrations of BAP, TDZ and NAA and produced yellow to green friable and compact and dense calli. The maximum amount of callus per explants was produced on MS medium with 4.44 µM of BAP, 1.16 µM of TDZ and 1.135 µM of NAA combination and concentration gave the 84% response and produced green compact calli, 19.98 ± 0.03 shoots from the callus and the maximum mean number of ischolar_mainlets per culture was also noted (15 ± 0.57) in this combination with MS medium of half strength. The in vitro developed entire plantlets were fruitfully acclimatized to thermocol cups and earthen pots.

Keywords


Callogenesis, Capparidaceae, Cleome monophylla, Growth Regulators, Shoot and Root Induction

References





DOI: https://doi.org/10.15613/sijrs%2F2018%2Fv5i2%2F207621