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Toxicity Evaluation of Aqueous Extracts of Allium cepa and Coriandrum Sativum in Rats
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Objectives: Allium cepa and Coriandrum sativum are reported in the Ayurvedic literature for hypolipidemic activities. Aqueous extracts of A. cepa and C. sativum prior to the assessment of the efficacy of the extracts for antiobesity potential were investigated for the safety of acute oral exposure in rats. Materials and Methods: Aqueous extracts of A. cepa or C. sativum was administered orally in a sequential manner to one animal each at 2000 and 5000 mg/kg limit test of sighting study followed by four animals at 5000 mg/kg in the main study as per Organisation for Economic Cooperation and Development guideline 420. The experimental animals were observed for mortality, adverse clinical signs, alterations in the body weights, and gross pathological lesions at necropsy. Similar procedure was adopted for the other test substance. Results: Aqueous extracts of A. cepa or C. sativum treated rats survived throughout the study period and did not exhibit any treatment-related adverse clinical signs at the tested dose levels. The overall percent body weight gain in rats treated with aqueous extracts of A. cepa or C. sativum was found to be normal during the 14 days observation period. At necropsy, the rats did not reveal any major pathological lesions. Conclusion: In summary, acute oral toxicity testing of aqueous extracts of A. cepa and C. sativum did not demonstrate any treatment-related adverse effects up to the dose level of 5000 mg/kg body weight, and hence the tested products were labeled unclassified in the hazard category according to Globally Harmonized System.
Keywords
Acute Oral Toxicity, Allium cepa, Coriandrum sativum, Organisation for Economic Cooperation and Development, Rats.
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- Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 4:177.
- Pelkonen O, Xu Q, Fan TP. Why is research on herbal medicinal products important and how can we improve its quality? J Tradit Complement Med. 2014; 4:1–7.
- Rivera JO, Loya AM, Ceballos R. Use of herbal medicines and implications for conventional drug therapy medical sciences. Altern Integr Med. 2013; 2:6.
- Hussain K, Majeed MT, Ismail Z, Sadikun A, Ibrahim P. Traditional and complementary medicines: Quality assessment strategies and safe usage. South Med Rev. 2009; 2:19–23.
- Wachtel-Galor S, Benzie FF, editors. An introduction to its history, usage, regulation, current trends, and research needs. In: Herbal Medicine: Biomolecular and Clinical Aspects. 2nd ed. Boca Raton (FL): CRC Press; 2011. p. 13.
- Joshi SC, Jain PK. A review on hypolipidaemic and antioxidant potential of some medicinal plants. World J Pharm Pharm Sci. 2014; 3:357–80.
- Bora KS, Sharma A. Phytoconstituents and therapeutic potential of Allium cepa Linn- A review. Pharmacogn Rev. 2009; 3:159–69.
- Bang MA, Kim HA, Cho YJ. Alterations in the blood glucose, serum lipids and renal oxidative stress in diabetic rats by supplementation of onion (Allium cepa. Linn). Nutr Res Pract. 2009; 3:242–6.
- Abou El-Soud N, Khalil M. Antioxidative effects of Allium cepa essential oil in streptozotocin induced diabetic rats. Macedonian J Med Sci. 2010; 3:344–51.
- Sahib NG, Anwar F, Gilani AH, Hamid AA, Saari N, Alkharfy KM. Coriander (Coriandrum sativum L.): A potential source of high-value components for functional foods and nutraceuticals- A review. Phytother Res. 2013; 27:1439–56.
- Organization for Economic Co-operation and Development (OECD). OECD Guideline for Testing of Chemicals: Guideline 420, Acute Oral Toxicity- Fixed Dose Procedure, OECD Paris; France. 2001a. Available from: http://www.ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl420.pdf
- Cheung BM, Cheung TT, Samaranayake NR. Safety of antiobesity drugs. Ther Adv Drug Saf. 2013; 4:171–81.
- Lee WJ, Kim HW, Lee HY, Son CG. Systematic review on herb-induced liver injury in Korea. Food Chem Toxicol. 2015; 84:47–54.
- Stokes WS. Humane endpoints for laboratory animals used in regulatory testing. ILAR J. 2002; 43(Suppl):S31–8.
- Organization for Economic Co-operation and Development
- (OECD). Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation. Environmental Health and Safety Monograph Series on Testing and Assessment No19, OECD; Paris, France. 2000. Available from: http://www.imm.ki.se/sft/pdf/OECD19.pdf
- Organization for Economic Co-operation and Development (OECD). OECD Guideline for Testing of Chemicals: Guideline 423, Acute Oral Toxicity – Acute Toxic Class Method, OECD; Paris, France. 2001b. Available from: http://www.ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf
- Organization for Economic Co-operation and Development (OECD). OECD Guideline for Testing of Chemicals: Guideline 425, Acute Oral Toxicity – Up-and-Down Procedure, OECD; Paris, France. 2001c. Available from: http://www.oecd.org/dataoecd/17/51/1948378.pdf
- Johnson MD, Gad SC. The rat. In: Gad SC, editor. Animal Models in Toxicology. 2nd ed. Boca Raton: CRC Press; 2007. p. 193–5.
- Frame SR, Mann PC, Rae MJ. Principles of pathology for toxicological studies. In: Hayes AW, Kruger CL, editors. Principles and Methods of Toxicology. 5th ed. United States: RC Press; 2007. p. 592.
- Kojima T, Tanaka T, Mori H, Kato Y, Nakamura M. Acute and subacute toxicity tests of onion coat, natural colorant extracted from onion (Allium cepa L.), in (C57BL/6 x C3H)F1 mice. J Toxicol Environ Health. 1993; 38:89–101.
- Bhanot A, Shri R. A comparative profile of methanol extracts of Allium cepa and Allium sativum in diabetic neuropathy in mice. Pharmacognosy Res. 2010; 2:374–84.
- Patel D, Desai S, Devkar R, Ramachandran AV. Acute and sub-chronic toxicological evaluation of hydro-methanolic extract of Coriandrum sativum l. Seeds. EXCLI J 2012; 11:566–75.
- Burdock GA, Carabin IG. Safety assessment of coriander (Coriandrum sativum L.) essential oil as a food ingredient. Food Chem Toxicol. 2009; 47:22–34.
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