Toxicology International (Formerly Indian Journal of Toxicology)

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Toxic Effects of Quinalphos on Protein Content and Growth Rate in Freshwater Teleost, Cyprinus carpio (L.)


Affiliations
1 Department of Aquatic Environment Management, College of Fisheries, Mangalore, India
2 Department of Veterinary Pharmacology and Toxicology, Veterinary College, Bangalore, India
3 Department of Aquaculture, College of Fisheries, Mangalore, India
     

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Organophosphate based pesticides account for a major percentage of pesticides used in domestic, agricultural and industrial applications throughout the world. They are highly popular because they are effective, non persistent and relatively less expensive. Quinalphos is a synthetic organophosphate which has become a matter of concern because of its potentiality and hazardous effect. Hence, the present investigation was designed to assess the impact of quinalphos on total protein content in the selected tissues and growth rate of freshwater fish, Cyprinus carpio. The lethal toxicity of quinalphos to the fish exposed for 96 h was found to be 2.75 ppm. For sublethal toxicity study, the fish were exposed to two concentration viz., 1/5th of LC50 (0.550 ppm) and 1/10th of LC50 (0.275 ppm) along with control as reference for 4, 8, 16 and 32 days. Decrease in total protein concentration and growth rate were observed in 4th and 8th day of exposure, but on 16th and 32nd day of exposure all the values reached nearer to normal condition. The decline in total protein levels and relative growth rate at initial exposure periods may indicates the high energy demand associated with imposed quinalphos stress. Restoration of protein fractions to normal, implies that after 8 days of exposure there seems to exist an oscillatory phase in protein turnover towards a more synthetic phase leading to the establishment of recuperation and adaptation phenomena.

Keywords

Quinalphos, Toxicity, Freshwater Teleost, Total Protein, Growth.
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  • Joshi PP, Kulkarni GK. Cypermethrin and fenvalerate induced protein alterations in freshwater crab, Barytelphusa cunicularis (Westwood). Recent Res Sci Tech 2011; 3(12):710.

  • Varo I, Serrano R, Pitarch E, Amat F, Lopez FJ, Navarro JC. Bioaccumulation of chlorpyrifos through an experimental food chain: study of protein HSP70 as biomarker of sublethal stress in fish. Arch Environ Contam Toxicol 2002; 42:229–235.

  • Gad NS, Saad AS. Effect of environmental pollution by phenol on some physiological parameters of Oreochromis niloticus. Global Veterinaria 2008; 2(6):312-319.

  • Huang DJ, Chen HC. Oxygen consumption, ammonia-N excretion and growth rate in juvenile green neon shrimp (Neocaridina denticulata) exposed to chlordane and lindane. Acta Zool Taiwan 2004; 14(2):65-76.

  • Das BK, Mukherjee SC. Sublethal effects of quinalphos on selected blood parameters of Labeo rohita (Ham) fingerlings. Asian Fish Sci 2000; 13:225-233.

  • Cengiz EI, Unlu E. Histopathology of gills in mosquito fish, Gambusia affinis after long-term exposure to sublethal concentrations of malathion. J Environ Sci Health 2003; 38(B):581-589.

  • Triebskorn R, Kohler HR, Honnen W, Schramm M, Adams SM. Induction of heat shock proteins, changes in liver ultrastructure and alterations of fish behavior: Are these biomarkers related and are they useful to reflect the state of pollution in the field? J. Aquat Ecosyst Stress Recovery 1997; 6:57-73.

  • Pereira P, De-Pablo H, Pacheco M, Vale C. The relevance of temporal and organ specific factors on metals accumulation and biochemical effects in feral fish (Liza aurata) under a moderate contamination scenario. Ecotoxicol Environ Saf 2010; 73:805-816.

  • APHA, AWWA, WPCF. Standard methods for the examination of water and wastewater. Washington, DC: American Public Health Association 1981; pp 1134.

  • Finney DJ. Probit Analysis, 3rd Ed. Cambridge University Press, London 1971; 330 pp.

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193:265-275.

  • Duncan DB. Multiple range and multiple F – tests. Biometrics 1955; 11:1-42.

  • Snedecor GW, Cochran GW. Statistical Methods. Oxford and IBH Publishing Company, Calcutta, India 1968.

  • Ogueji EO, Auta J. Investigation of biochemical effects of acute concentration of lambda-cyhalothrin on African catfish, Clarias gariepinus (Teguels). J Fish Intern 2007; 2(1):86-90.

  • Chebbi SG, David M. Modulation in the protein metabolism under sublethal concentration of quinalphos intoxication in the freshwater common carp, Cyprinus carpio (Linnaeus, 1758). Int J Pharm Biol Arch 2011; 2(4):1183-1189.

  • Magar RS, Shaikh A. Biochemical changes in proteins and amino acids in Channa punctatus in responses to sublethal treatment with the insecticide malathion. Trends in Life Sci 2012; 1(3):2319–4731.

  • Singh SP, Singh S. Metabolic effects of an organophosphorus dipterex in Trichogaster fasciatus. Ind J Biol Res 2011; 1(1):61-66.

  • Fahmy GH. Malathion toxicity: Effect of some metabolic activities in Oreochromis niloticus, the Tilapia fish. Int J Bio Biochem Bioinf 2012; 2(1):52-55.

  • Vidhya V, Nair CR. Lambda-cyhalothrin induced protein alternations in Etroplus suratensis (Bloch). Global J Pharmacol 2013; 7(2):166-171.

  • Neeraja SRK, Giridhar P. Effect of deltamethrin on some aspects of protein metabolism in fresh water fish Labeo rohita (Hamilton). Int J Pharm Pharm Sci 2014; 6(7):494-497.

  • Dixon DG, Sprague JB. Copper bioaccumulation and hepato protein synthesis during acclimation to copper by juvenile rainbow trout. Aquatic Toxicol 1981; 1:69-81.

  • Kito H, Osc Y, Sato T, Ishikawa T. Formation of metalloprotein in fish. Comp Biochem Physiol 1982; 73C (1):129-34.

  • Umminger BL. Physiological studies on super cooled kill fish, Fundus heteroclitus. III. Carbohydrate metabolism and survival at subzero temperature. J Exp Zool 1970; 173:15974.

  • Young UR. In: Mammalia protein metabolism (H.N. Mumo. Ed), Academic Press. New York 1970; 4:485.

  • Rekha P, John J. Study on the changes in the levels of protein metabolism in λ cyhalothrin induced hepatotoxicity in fresh water Tilapia (Oreochromis mossambicus). Adv Appl Sci Res 2011; 2(3):57-62.

  • Heath AG. Use of physiological and biochemical measures in pollution biology. In: Water pollution and fish physiology. Lewis Publishers, CRC Press, Baco Raton, FL 1995; pp 325-342.

  • Miliou H, Zaboukas N, Moraitou-Apostolopoulou M. Biochemical composition, growth and survival of the guppy, Poecilia reticulata during chronic sublethal exposure to cadmium. Arch Environ Contam Toxicol 1998; 35:5863.

  • Moraes FD, De FP, Venturini LRX, Cortella PA, Rossi, Moraes G. Acute toxicity of pyrethroid-based insecticides in the Neotropical freshwater fish, Brycon amazonicus. Ecotoxicol Environ Contam 2013; 8(2):59-64.

  • Kim SG, Kang JC. Effect of dietary copper exposure on accumulation, growth and hematological parameters of the juvenile rockfish, (Sebstes schlegeli). Mar Environ Res 2004; 58:65-82.

  • Hayat S, Javed M, Razzaq S. Growth performance of metal stressed major carps viz. Catla catla, Labeo rohita and Cirrhina mrigala reared under semi-intensive culture system. Pakistan Vet J 2007; 27:8-12.

  • Cleveland L, Hamilton SJ. Toxicity of the organophosphorus defoliant to rainbow trout, Salmo gairdneri and channel catfish Ictalurus punctatus. Aquat Toxicol 1983; 4:341-355.

  • Brocksen RW. Influence of competition on the food consumption and production of animals in laboratory stream communities. MS thesis, Oregon State University, Corvallis, OR 1966; 82 pp.

  • Woltering DM. The growth response in fish chronic and early life stage toxicity tests: a critical review. Aquat Toxicol 1984; 5:1–21.


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  • Toxic Effects of Quinalphos on Protein Content and Growth Rate in Freshwater Teleost, Cyprinus carpio (L.)

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Authors

A. Padmanabha
Department of Aquatic Environment Management, College of Fisheries, Mangalore, India
H. R. V. Reddy
Department of Aquatic Environment Management, College of Fisheries, Mangalore, India
N. B. Shridhar
Department of Veterinary Pharmacology and Toxicology, Veterinary College, Bangalore, India
Muttappa Khavi
Department of Aquatic Environment Management, College of Fisheries, Mangalore, India
B. T. Naveen Kumar
Department of Aquaculture, College of Fisheries, Mangalore, India

Abstract


Organophosphate based pesticides account for a major percentage of pesticides used in domestic, agricultural and industrial applications throughout the world. They are highly popular because they are effective, non persistent and relatively less expensive. Quinalphos is a synthetic organophosphate which has become a matter of concern because of its potentiality and hazardous effect. Hence, the present investigation was designed to assess the impact of quinalphos on total protein content in the selected tissues and growth rate of freshwater fish, Cyprinus carpio. The lethal toxicity of quinalphos to the fish exposed for 96 h was found to be 2.75 ppm. For sublethal toxicity study, the fish were exposed to two concentration viz., 1/5th of LC50 (0.550 ppm) and 1/10th of LC50 (0.275 ppm) along with control as reference for 4, 8, 16 and 32 days. Decrease in total protein concentration and growth rate were observed in 4th and 8th day of exposure, but on 16th and 32nd day of exposure all the values reached nearer to normal condition. The decline in total protein levels and relative growth rate at initial exposure periods may indicates the high energy demand associated with imposed quinalphos stress. Restoration of protein fractions to normal, implies that after 8 days of exposure there seems to exist an oscillatory phase in protein turnover towards a more synthetic phase leading to the establishment of recuperation and adaptation phenomena.

Keywords


Quinalphos, Toxicity, Freshwater Teleost, Total Protein, Growth.

References





DOI: https://doi.org/10.22506/ti%2F2016%2Fv23%2Fi1%2F146675