Toxicology International (Formerly Indian Journal of Toxicology)

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Effects of Residual Tobacco Smoke in A549, Hep-2 and 3T3 Cells


Affiliations
1 Graduate Program in Pathology, Federal University of Health Sciences of Porto Alegre, UFCSPA, Brazil
2 Feevale University, Brazil
3 Cytotoxicity Laboratory, Institute of Health Sciences, Feevale University, Brazil
4 Pharmacosciences Department, UFCSPA, Brazil
     

   Subscribe/Renew Journal


The aim of this study was to assess the effects of residual tobacco smoke in the A549, Hep-2 and 3T3 cells. Cellulose papers were exposed to the smoke of 1, 3, 5 and 8 cigarettes. Cotton wipes were used to clean the chamber walls after extinction of the smoke. The paper and cotton were immersed in Dulbecco's Modified Eagle Media (DMEM) to transfer the compounds of the material to the solvent. DMEM+THS was used to expose A549, Hep-2 and 3T3 cells for 24 hours and MTT, Neutral Red Uptake (NRU) and Trypan blue exclusion assays were performed. No significantly changes were found in A549 cells. In Hep-2 cells, there was difference only in NRU assays. In 3T3 cells, changes in mitochondrial activity and proliferation rate were observed. Because of different sensibility of cells to THS, it is important to screen multiple cell types in order to better assess cytotoxicity.

Keywords

Thirdhand Tobacco Smoke, Environmental Tobacco Smoke, MTT Assay, Neutral Red Uptake Assay.
User
Subscription Login to verify subscription
Notifications
Font Size

  • Winickoff JP, Friebely J, Tanski SE, Sherrod C, Matt GE, Hovell MF, et al. Beliefs about the health effects of “thirdhand” smoke and home smoking bans. Pediatrics 2009 Jan;123(1):e74-9. doi: 10.1542/peds.2008-2184.

  • Burton, A., 2011. Burton A. Does the smoke ever really clear? Thirdhand smoke exposure raises new concerns. Environ Health Perspect 2011 Feb;119(2):A70-4.doi: 10.1289/ehp.119-a70.

  • Sleiman M, Gundel LA, Pankow JF, Jacob P 3rd, Singer BC, Destaillats H. Formation of carcinogens indoors by surface-mediated reactions of nicotine with nitrous acid, leading to potential thirdhand smoke hazards. ProcNatlAcadSci U S A 2010a;107(15):6576-81.doi: 10.1073/pnas.0912820107.

  • Hoh E, Hunt RN, Quintana PJ, Zakarian JM, Chatfield DA, Wittry BC, et al. Environmental tobacco smoke as a source of polycyclic aromatic hydrocarbons in settled household dust. Environ SciTechnol 2012 Apr 3;46(7):4174-83.doi: 10.1021/es300267g.

  • Bahl V, Jacob P 3rd, Havel C, Schick SF, Talbot P. Thirdhand cigarette smoke: factors affecting exposure and remediation. PLoS One 2014 Oct 6;9(10):e108258. doi: 10.1371/journal.pone.0108258.

  • Bahl V, Shim HJ, Jacob P 3rd, Dias K, Schick SF, Talbot P. Thirdhand smoke: Chemical dynamics, cytotoxicity, and genotoxicity in outdoor and indoor environments. ToxicolIn Vitro 2015a Dec 9. pii: S0887-2333(15)30028-X. doi: 10.1016/j.tiv.2015.12.007.

  • Matt GE, Quintana PJ, Zakarian JM, Fortmann AL, Chatfield DA, Hoh E, et al. When smokers move out and non-smokers move in: residential thirdhand smoke pollution and exposure. Tob Control 2011a Jan;20(1):e1.doi: 10.1136/tc.2010.037382.

  • Hang B, Sarker AH, Havel C, Saha S, Hazra TK, Schick S, et al. Thirdhand smoke causes DNA damage in human cells. Mutagenesis 2013 Jul;28(4):381-91.doi: 10.1093/mutage/get013.

  • Ramirez N, Ozel MZ, Lewis AC, Marcé RM, Borrull F, Hamilton JF. Exposure to nitrosamines in thirdhand tobacco smoke increases cancer risk in non-smokers. Environ Int2014;71:139-47.doi: 10.1016/j.envint.2014.06.012.

  • Xu B, Chen M, Yao M, Ji X, Mao Z, Tang W, et al. Metabolomics reveals metabolic changes in male reproductive cells exposed to thirdhand smoke. Sci Rep 2015 Oct 22;5:15512.doi: 10.1038/srep15512.

  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983 Dec 16;65(1-2):55-63.

  • Trintinaglia L, Bianchi E, Silva LB, Nascimento CA, Spilki FR, Ziulkoski AL. Cytotoxicity assays as tools to assess water quality in the Sinos river basin. Braz J Biol 2015 May;75(2 Suppl):75-80. doi: 10.1590/1519-6984.0113.

  • Borenfreund E, Puerner JA. Toxicity determined in vitro by morphological alterations and neutral red absorption. ToxicolLett 1985 Feb-Mar;24(2-3):119-24.

  • Altman SA, Randers L, Rao G. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations. BiotechnolProg 1993 Nov-Dec;9(6):671-4.

  • International Agency for Research on Cancer - IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Tobacco Smoke and Involuntary Smoking. IARC MonogrEvalCarcinog Risks Hum 2004;83:1-1438.

  • Hammer TR, Fischer K, Mueller M, Hoefer D. Effects of cigarette smoke residues from textiles on fibroblasts, neurocytes and zebrafish embryos and nicotine permeation through human skin. Int J Hyg Environ Health 2011 Sep;214(5):384-91. doi: 10.1016/j.ijheh.2011.04.007.

  • Amaro MI, MonasteriosM, Avendaño M, Charris J. Preliminary evaluation of the toxicity of some synthetic furan derivatives in two cell lines and Artemiasalina. J ApplToxicol 2009 Jan;29(1):36-41. doi: 10.1002/jat.1379.

  • Bahl V, Weng NJ, Schick SF, Sleiman M, Whitehead J, Ibarra A, et al. Cytotoxicity of Thirdhand Smoke and Identification of Acrolein as a Volatile Thirdhand Smoke Chemical That Inhibits Cell Proliferation. ToxicolSci 2015 Dec 29. pii: kfv327.

  • Sobus SL1, Warren GW. The biologic effects of cigarette smoke on cancer cells. Cancer 2014 Dec 1;120(23):3617-26. doi: 10.1002/cncr.28904.

  • Putnam KP1, Bombick DW, Doolittle DJ. Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. ToxicolIn Vitro 2002 Oct;16(5):599-607.

  • Wang H, Word B, Lyn-Cook L Jr, Yang M, Hammons G, Lyn-Cook B. Cytotoxicity of chronic exposure to 4 cigarette smoke condensates in 2 cell lines. Int J Toxicol 2015 Mar-Apr;34(2):182-94. doi: 10.1177/1091581815574349.

  • Riss TL, Moravec RA, Niles AL, Benink HA, Worzella TJ, Minor L. Cell Viability Assays. In: Sittampalam GS, Coussens NP, Nelson, H, et al., editors. Assay Guidance Manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences, Bethesda (MD): 2004. Retried: http://www.ncbi.nlm.nih.gov/books/NBK144065/. Accessed: Jan 28, 2016.

  • Fukano Y, Ogura M, Eguchi K, Shibagaki M, Suzuki M. Modified procedure of a direct in vitro exposure system for mammalian cells to whole cigarette smoke. ExpToxicolPathol 2004 Mar;55(5):317-23.


Abstract Views: 463

PDF Views: 1




  • Effects of Residual Tobacco Smoke in A549, Hep-2 and 3T3 Cells

Abstract Views: 463  |  PDF Views: 1

Authors

Luciana Rizzieri Figueiro
Graduate Program in Pathology, Federal University of Health Sciences of Porto Alegre, UFCSPA, Brazil
Amanda Ferreira Becker
Feevale University, Brazil
Ana Luiza Ziulkoski
Cytotoxicity Laboratory, Institute of Health Sciences, Feevale University, Brazil
Denise Conceicao Mesquita Dantas
Pharmacosciences Department, UFCSPA, Brazil

Abstract


The aim of this study was to assess the effects of residual tobacco smoke in the A549, Hep-2 and 3T3 cells. Cellulose papers were exposed to the smoke of 1, 3, 5 and 8 cigarettes. Cotton wipes were used to clean the chamber walls after extinction of the smoke. The paper and cotton were immersed in Dulbecco's Modified Eagle Media (DMEM) to transfer the compounds of the material to the solvent. DMEM+THS was used to expose A549, Hep-2 and 3T3 cells for 24 hours and MTT, Neutral Red Uptake (NRU) and Trypan blue exclusion assays were performed. No significantly changes were found in A549 cells. In Hep-2 cells, there was difference only in NRU assays. In 3T3 cells, changes in mitochondrial activity and proliferation rate were observed. Because of different sensibility of cells to THS, it is important to screen multiple cell types in order to better assess cytotoxicity.

Keywords


Thirdhand Tobacco Smoke, Environmental Tobacco Smoke, MTT Assay, Neutral Red Uptake Assay.

References





DOI: https://doi.org/10.22506/ti%2F2016%2Fv23%2Fi2%2F146695