Toxicology International (Formerly Indian Journal of Toxicology)

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Protective Effect of Naringin Flavonoid on Cisplatin Induced Hematotoxicity and Hepatotoxicity in Wistar Rats


Affiliations
1 Centre for Research and Development, PRIST University, Thanjavur, Tamilnadu, India
2 Pharmacology Department, Shree Devi College of Pharmacy, Mangalore, India
     

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Cisplatin is an important anticancer drug used in treatment of wide range of cancers. Even though it has got severe adverse effects which are linked with elevated oxidative stress. The objective of the present study was to investigate the possible protective effect of flavonoid Naringin against the oxidative stress induced by cisplatin (CP) on hematic parameters and hepatic antioxidant biomarkers in wistar rats. Animals were randomly divided into four equal groups in separate cages, six rats in each: control (saline 1ml p.o.), Cisplatin (7.5 mg/kg once i.p), combination of cisplatin and Naringin (50mg/kg p.o. for 21 days) and combination of cisplatin and Naringin (100mg/kg p.o. for 21 days). Blood samples were collected and subjected for evaluation of haematological parameters (RBC, WBC, Hb, and Platelets) and serum biomarkers (ALT, AST & ALP). Livers were excised from rats and subjected for antioxidants (GSH, MDA, SOD&Catalase) evaluation and histopathological study. Naringin provided a beneficial (P<0.001) role in CP-induced hemotoxicity by increasing RBC, Hb, PCV & platelets. Naringin also showed significant (P<0.001) protection against CP induced hepatotoxicity by decreasing ALT, AST, ALP, MDA level and by increasing the GSH, SOD, catalase, due to its ability to reduce free radical-induced oxidative damage in the liver and to scavenge the hydroxyl and peroxyl radicals. The present findings clearly suggest that Naringin protects against cisplatin induced oxidative stress on blood cells and hepatic antioxidant defence system.

Keywords

Naringin, Cisplatin, Haemotoxicity, Oxidative Stress, Hepatotoxicity.
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  • Mansour HH, Hafez HF and Fahmy NM. Silymarin modulates Cisplatin10mg/kg induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol 2006; 39: 656–61.

  • Kart A, Yilmaz C, Musa K & Hasan O. Caffeic acid phenethylester( CAPE) ameliorates cisplatin-induced hepatotoxicity in rabbit. Exp Toxicol Pathol 2010; 62: 45–52.

  • Iseri S, Ercan F, Gedik N, Yuksel M & Alican I. Simvastatin attenuates cisplatin-induced kidney and liver damage in rats. Toxicol 2007; 230(2): 256–64.

  • Abdelmeguid NE, Chmaisse HN, Abou Zeinab NS. Silymarin ameliorates cisplatin-induced hepatotoxicity in rats: histopathological and ultra structural studies. Pak J Biol Sci 2010(13): 463–79.

  • James MH, Robert FD, Gary RB, Joanne MH, David BH, Seema B et al. Flavonoid Content of U.S. Fruits, Vegetables, and Nuts. J Agric Food Chem 2006; 54: 9966-77.

  • Agarwal AD. Pharmacological activities of Flavonoids – A review. Int J Pharm Nanotech 2011; 4(2): 1394-8.

  • Vishnuvarthan VJ, Srividya AR, Satishkumar MN. Role of Naringin and Naringinin on various diseased conditions- A review. Int J Pharm Res Schol 2013; 4(2): 198-210.

  • Caccamese S, Chillemi R. “Racemization at C-Z of Naringin in Pummela (citrus grandis) with increasing maturity determined by chiral high performance liquid chromatography”. J Chromat 2010; 1217:1089-93.

  • Ashraf YN. Protective effect of aged garlic extract against the oxidative stress induced by cisplatin on blood cells parameters and hepatic antioxidant enzymes in rats. Toxicol Rep 2014; 1:682–91.

  • Saharan RK and Sharma SC. Correlation Studies of trehalose with oxidative stress in ethanol stressed yeast pachysolen tannophilus. Curr Res J Biol Sci 2010; 2(5):300-5.

  • Mihara M, Uchiyama M. Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978; 86 (1): 271–8.

  • Ellmann GL. Tissue sulfhydril groups. Arch Biochem Biophys 1959; 82:70-7.

  • Aebi H, Catalase, in: H.U. Bergmeyer (Ed.), Methods in Enzymatic Analysis, vol. 3, Academic Press, New York, 1983, pp. 276–86.

  • Kakkar PS, Das B, Viswanathan PNA. Modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984; 21:130–2.

  • Bancroft JD, Gamble M. Theory and Practice of Histological Techniques, 5th ed., Churchill Livingstone Pub., Edinburgh/ New York/London/Philadelphia, 2002, pp. 125–38, 172–5, 184–93,593–620.

  • Amany AT, Ahmed MA, Ahmed EAM and Romissaa HS. Cinnamic acid Attenuates Cisplatin-Induced Hepatotoxicity and Nephrotoxicity. J Bas Environ Sci 2016; 3:1-9.

  • Arhoghro EM, IKeh C and Prohp TP. Cymbopogon citratus aqueous extract alleviates cisplatin-induced hepatic oxidative stress and toxicity in albino rats. Int J Curr Microbiol App Sci 2014; 3(4): 586-604.

  • Yamini C, Chetenchery SP. Therapeutic efficacy of naringin on cyclosporine (A) induced nephrotoxicity in rats: Involvement of hemeoxygenase-1. Pharmacological Reports 2013; 65: 1336-44.

  • Yuan G et al. Toxicological assessment of combined lead and cadmium: acute and sub-chronic toxicity study in rats, Food Chem Toxicol. 2014; 65:260–8.

  • Sirage HM. Biochemical and hematological studies for the protective effect of Oyster Mushroom (Pleurotus ostreatus) against glycerol-induced acute renal failure in rats. J Biol Sci 2009; 9(7): 746–52.

  • Olas B, Wachowicz B, Majsterek I, Blasiak J. Resveratrol may reduce oxidative stress induced by platinum compounds in human plasma, blood platelets and lymphocytes. Anticancer Drugs 2005; 16:659–65.

  • Yang X et al. Naringin administration inhibits platelet aggregation and release by reducing blood cholesterol levels and the cytosolic free calcium concentration in hyperlipidemic rabbits. Exp Therap Medicine 2014; 8: 968-72.

  • Heba HM, Hafez FH and Nadia MF. Silymarin Modulates Cisplatin-Induced Oxidative Stress and Hepatotoxicity in Rats. J Biochem Mol Biol 2006; 39(6): 656-61.

  • El-Sharaky AS, Newairy AA, Kamel MA, Eweda SM. Protective effect of ginger extract against bromobenzene induced hepatotoxicity in male rats. Food Chem Toxicol 2009;47: 1584–90.

  • S. Shona, E. Abdel-Wakeel, M. Sherif, Zaki. Tarek, I. AbdelGalil, Effectof cisplatinum on the liver of the adult albino rat and the possible protective role of vitamin E (Histological and Ultrastructural Study). Anat Physiol 2012; 2(3):1–5.

  • Osama MA, Basant MM, Asmaa MM. Curcumin and Naringin prevents 7,12-Dimethyl benz(A) anthrecene induced hepatic injury by suppressing inflammation and oxidative stress. J Int Acad Res Multidisciplinery 2014;2(4): 589-603.


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  • Protective Effect of Naringin Flavonoid on Cisplatin Induced Hematotoxicity and Hepatotoxicity in Wistar Rats

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Authors

Sanjiv Karale
Centre for Research and Development, PRIST University, Thanjavur, Tamilnadu, India
Jagadish V. Kamath
Pharmacology Department, Shree Devi College of Pharmacy, Mangalore, India

Abstract


Cisplatin is an important anticancer drug used in treatment of wide range of cancers. Even though it has got severe adverse effects which are linked with elevated oxidative stress. The objective of the present study was to investigate the possible protective effect of flavonoid Naringin against the oxidative stress induced by cisplatin (CP) on hematic parameters and hepatic antioxidant biomarkers in wistar rats. Animals were randomly divided into four equal groups in separate cages, six rats in each: control (saline 1ml p.o.), Cisplatin (7.5 mg/kg once i.p), combination of cisplatin and Naringin (50mg/kg p.o. for 21 days) and combination of cisplatin and Naringin (100mg/kg p.o. for 21 days). Blood samples were collected and subjected for evaluation of haematological parameters (RBC, WBC, Hb, and Platelets) and serum biomarkers (ALT, AST & ALP). Livers were excised from rats and subjected for antioxidants (GSH, MDA, SOD&Catalase) evaluation and histopathological study. Naringin provided a beneficial (P<0.001) role in CP-induced hemotoxicity by increasing RBC, Hb, PCV & platelets. Naringin also showed significant (P<0.001) protection against CP induced hepatotoxicity by decreasing ALT, AST, ALP, MDA level and by increasing the GSH, SOD, catalase, due to its ability to reduce free radical-induced oxidative damage in the liver and to scavenge the hydroxyl and peroxyl radicals. The present findings clearly suggest that Naringin protects against cisplatin induced oxidative stress on blood cells and hepatic antioxidant defence system.

Keywords


Naringin, Cisplatin, Haemotoxicity, Oxidative Stress, Hepatotoxicity.

References





DOI: https://doi.org/10.22506/ti%2F2016%2Fv23%2Fi2%2F146702