Chemopreventive Role of Euphorbia neriifolia (Linn) and its Isolated Flavonoid Against N-Nitrosodiethylamine-induced Renal Histopathological Damage in Male Mice

Aims: This study is an attempt to evaluate the tissue protective efficacy of isolated flavonoid and hydro‑ethanolic extract of Euphorbia neriifolia (HEEN) leaves against N‑nitrosodiethylamine (DENA) induced renal carcinoma. Materials and Methods: Carcinogenicity was induced in Albino mice by oral administration of DENA (50 mg/kg body weight). The HEEN (150 and 400 mg/kg body weight), Butylated hydroxyanisole (BHA; 0.5 and 1%), and Euphorbia neriifolia flavonoid (ENF; 50 mg/kg body weight) were evaluated for their possible tissue carcinogenesis protective potential. Results: DENA treated animals showed alterations in normal renal histo‑architecture, which comprised of necrosis (N) and vacuolization of the cells. On the other hand, the mice treated with Euphorbia neriifolia lower (ENL) and higher (ENH) dose and ENF before intoxicated with DENA showed that the renal cells were normal (Day 31). Whereas, BHA higher (BHAH) and lower (BHAL) dose failed to diminish the abnormalities caused by DENA. Conclusions: The findings of the present study Suggested that ENH and ENF showed highest renal-protective activity among all the pretreatments. The results could also be expressed in the order of ENH > ENF > ENL > BHAH > BHAL.