Toxicology International (Formerly Indian Journal of Toxicology)

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Hemato-Biochemical Alterations Mediated by Carbendazim Exposure and Protective Effect of Quercetin in Male Rats


Affiliations
1 Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
2 Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
     

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The main objective of study was to investigate the effect of Carbendazim (CBZ) a most popular agriculture fungicide on hemato-biochemical alterations in male rats and possible ameliorative effect of quercetin. Sprague Dawley male rats were administered CBZ @100, 200 and 400 mg/kg body weight, PO and quercetin (25mg/kg body weight, PO) alone and concomitant in corn oil for the period of 28 days. At the end of exposure period, blood was collected and hemato-biochemical parameters were evaluated in blood and plasma samples. Sub-acute exposure of carbendazim resulted in non-significant decrease (p > 0.05) in haemoglobin, PVC and TEC and increase in MCV, MCH and platelets count in CBZ treated groups. Moreover, administration of CBZ significantly increased (p < 0.05) the plasma ALT, AST, ALP, cholesterol and triglyceride while, significantly decreased (p < 0.05) the plasma protein and globulin level was noticed. Non-significant change in calcium, chloride and phosphorous was observed in CBZ alone treated groups. However, quercetin supplementation concomitant in CBZ treated animals prevented and restored the alterations caused by exposure of CBZ. This study concluded that quercetin has ability to ameliorate the toxic effect caused by CBZ on hemato-biochemical biomarkers in living beings.

Keywords

Carbendazim, Haemato-Biochemical, Quercetin, Rats.
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  • Hemato-Biochemical Alterations Mediated by Carbendazim Exposure and Protective Effect of Quercetin in Male Rats

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Authors

N. V. Patil
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
M. K. Lonare
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
M. Sharma
Department of Veterinary Physiology and Biochemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
P. C. Lalhriatpuia
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
S. P. S. Saini
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India
S. Rampal
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana − 141004, Punjab, India

Abstract


The main objective of study was to investigate the effect of Carbendazim (CBZ) a most popular agriculture fungicide on hemato-biochemical alterations in male rats and possible ameliorative effect of quercetin. Sprague Dawley male rats were administered CBZ @100, 200 and 400 mg/kg body weight, PO and quercetin (25mg/kg body weight, PO) alone and concomitant in corn oil for the period of 28 days. At the end of exposure period, blood was collected and hemato-biochemical parameters were evaluated in blood and plasma samples. Sub-acute exposure of carbendazim resulted in non-significant decrease (p > 0.05) in haemoglobin, PVC and TEC and increase in MCV, MCH and platelets count in CBZ treated groups. Moreover, administration of CBZ significantly increased (p < 0.05) the plasma ALT, AST, ALP, cholesterol and triglyceride while, significantly decreased (p < 0.05) the plasma protein and globulin level was noticed. Non-significant change in calcium, chloride and phosphorous was observed in CBZ alone treated groups. However, quercetin supplementation concomitant in CBZ treated animals prevented and restored the alterations caused by exposure of CBZ. This study concluded that quercetin has ability to ameliorate the toxic effect caused by CBZ on hemato-biochemical biomarkers in living beings.

Keywords


Carbendazim, Haemato-Biochemical, Quercetin, Rats.

References





DOI: https://doi.org/10.18311/ti%2F2018%2F21569