Toxicology International (Formerly Indian Journal of Toxicology)

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Safety Evaluation and In-vitro Antioxidant Activity of Exopolysaccharide Produced by an Indigenous Species of Pleurotus pulmonarius RDM9


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1 Department of Microbiology, Panjab University, Chandigarh – 160014, India
     

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Higher fungus is capable of producing exopolysaccharides of different and unique sugar moieties. This property offers the use of EPS in various food and cosmetic applications. This study was designed to ensure that the EPS obtained from Pleurotus pulmonarius is safe for human consumption and is proposed to be used as a bioemulsifier and bioflocculant in food applications. Toxicological examination showed no sign of toxicity and mortality after treating BALB/c mice for consecutively 14 days by administering respective dose of EPS (100μl of sterilized PBS for control group and a dose of 50-400 mg EPS/kg in a constant volume of 100μl PBS for the test groups) via orogastric gavage. No significant change was recorded in body mass, morphology and behaviour of animals. Both ALT and AST activity were not affected by EPS in treated mice and hence the liver physiology was found to be normal. Histopathological examination of liver, kidney and small intestine showed no significant change in the morphology of the organs. The antioxidant capacity of purified EPS from P. pulmonarius was studied in in-vitro conditions by studying different biochemical methods and the results revealed that the test EPS showed a strong total antioxidant capacity and H2O2 scavenging activity.

Keywords

Antioxidant Activity, Exopolysaccharide, Pleurotus pulmonarius, White Rot Fungi.
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  • Safety Evaluation and In-vitro Antioxidant Activity of Exopolysaccharide Produced by an Indigenous Species of Pleurotus pulmonarius RDM9

Abstract Views: 250  |  PDF Views: 1

Authors

Deepika Malik
Department of Microbiology, Panjab University, Chandigarh – 160014, India
Deepak K. Rahi
Department of Microbiology, Panjab University, Chandigarh – 160014, India
Vijay Prabha
Department of Microbiology, Panjab University, Chandigarh – 160014, India

Abstract


Higher fungus is capable of producing exopolysaccharides of different and unique sugar moieties. This property offers the use of EPS in various food and cosmetic applications. This study was designed to ensure that the EPS obtained from Pleurotus pulmonarius is safe for human consumption and is proposed to be used as a bioemulsifier and bioflocculant in food applications. Toxicological examination showed no sign of toxicity and mortality after treating BALB/c mice for consecutively 14 days by administering respective dose of EPS (100μl of sterilized PBS for control group and a dose of 50-400 mg EPS/kg in a constant volume of 100μl PBS for the test groups) via orogastric gavage. No significant change was recorded in body mass, morphology and behaviour of animals. Both ALT and AST activity were not affected by EPS in treated mice and hence the liver physiology was found to be normal. Histopathological examination of liver, kidney and small intestine showed no significant change in the morphology of the organs. The antioxidant capacity of purified EPS from P. pulmonarius was studied in in-vitro conditions by studying different biochemical methods and the results revealed that the test EPS showed a strong total antioxidant capacity and H2O2 scavenging activity.

Keywords


Antioxidant Activity, Exopolysaccharide, Pleurotus pulmonarius, White Rot Fungi.

References





DOI: https://doi.org/10.18311/ti%2F2018%2F22509