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Immunomodulating Effects of Allium sativum in Renal Tissue of LPS Challenged Mice
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Allium sativum contain various medicinal properties like immunomodulatory, antitumorigenetic, antiatherosclerotic and antimicrobial. In the present study, we analyzed the impact of hydroethanol extract of Allium sativum on cytokine production in LPS challenged mice. LPS significantly induced the activation of NF-kB and also increased the levels of NO, TNF-α, IL-6, IFN-Y, PGE2 with the suppression of IL-10 production. In the results we found that Allium sativum significantly attenuated the LPS-induced phosphorylation of NF-ĸB and normalized the levels of NO, TNF-α, IL-6, IFN-Y, PGE-2 and IL-10. In summary, it was deduced that garlic may promote an anti-inflammatory effect by inhibition of NF-kB activity in the tissue.
Keywords
Allium sativum, Anti-inflammatory, Cytokines, Lipopolysaccharide (LPS), NF-kB.
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- Kabanov DS, Vwedenskaya OY, Fokina MA, Morozova EM, Grachev SV, Prokhorenko IR. Impact of CD14 on reactive oxygen species production from human leukocytes primed by Escherichia coli lipopolysaccharides. Oxidative Medicine and Cellular Longevity. 2019. PMid: 30944694 PMCid: PMC6421816. https://doi.org/10.1155/2019/6043245
- Gholamnezhad Z, Hassanabad ZF. Effects of lipopolysaccharide-induced septic shock on rat isolated kidney, possible role of nitric oxide and protein kinase C pathways. Iranian Journal of Basic Medical Sciences. 2018 Oct; 21(10):1073.
- Minasyan H. Sepsis: Mechanisms of bacterial injury to the patient. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine. 2019 Dec; 27(1):19. PMid: 30764843 PMCid: PMC6376788. https://doi.org/10.1186/s13049019-0596-4
- Imo C, Za’aku JS. Medicinal properties of ginger and garlic: A review. Current Trends of Biomedical Engineering and Biosciences. 2019; 18:2.
- Puccinelli M, Stan S. Dietary bioactive diallyl trisulfide in cancer prevention and treatment. International Journal of Molecular Sciences. 2017 Aug; 18(8):1645. PMid: 28788092 PMCid: PMC5578035. https://doi.org/10.3390/ijms18081645
- Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and [15N] nitrate in biological fluids. Analytical Biochemistry. 1982 Oct; 126(1):131–8. https://doi.org/10.1016/0003-2697(82)90118-X
- Mc Manus FA, Mowry RW. Staining methods: In histology and histochemistry. (P.B. Hoeber Ed). New York: Harper and Brothers; 1965.
- Patel NS, Chatterjee PK, Di Paola R, Mazzon E, Britti D, De Sarro A, Cuzzocrea S, Thiemermann C. Endogenous interleukin-6 enhances the renal injury, dysfunction and inflammation caused by ischemia/reperfusion. J Pharmacol Exp Ther. 2005; 312:1170–8. PMid: 15572648. https://doi.org/10.1124/jpet.104.078659
- Shin JH, Ryu JH, Kang MJ, Hwang CR, Han J, Kang D. Shortterm heating reduces the anti-inflammatory effects of fresh raw garlic extracts on the LPS-induced production of NO and pro-inflammatory cytokines by downregulating allicin activity in RAW 264.7 macrophages. Food and Chemical Toxicology. 2013 Aug; 58:545–51. PMid: 23583806. https://doi.org/10.1016/j.fct.2013.04.002
- Xiao ZY, Zheng QY, Jiang YY, Zhou B, Yin M, Wang HB, Zhang JP. Effects of esculentoside A on production of interleukin-1, 2 and prostaglandin E~ 2. Acta Pharmacologica Sinica. 2004 Jun; 25(6):817-21.
- Marquez Martin A, De La Puerta Vazquez R, FernandezArche A, Ruiz-Gutierrez V. Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages. Free Radical Research. 2006 Jan; 40(3):295–302. PMid: 16484046. https://doi.org/10.1080/10715760500467935
- Heba G, Krzemiński T, Porc M, Grzyb J, Ratajska A, Dembinska-Kiec A. The time course of tumor necrosis factor-α, inducible nitric oxide synthase and vascular endothelial growth factor expression in an experimental model of chronic myocardial infarction in rats. Journal of Vascular Research. 2001; 38(3):288–300. PMid: 11399901. https://doi.org/10.1159/000051057
- Salvemini D, Ischiropoulos H, Cuzzocrea S. Roles of nitric oxide and superoxide in inflammation. InInflammation Protocols. 2003. p. 291–303). Humana Press. PMid: 12769497. https://doi.org/10.1385/1-59259-374-7:291
- Funk CD, Funk LB, Kennedy ME, Pong AS, Fitzgerald GA. Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression and gene chromosomal assignment. The FASEB Journal. 1991 Jun; 5(9):2304–12. PMid: 1907252. https://doi.org/10.1096/fasebj.5.9.1907252
- Subbaramaiah K, Dannenberg AJ. Cyclooxygenase 2: A molecular target for cancer prevention and treatment. Trends in Pharmacological Sciences. 2003 Feb; 24(2):96–102. https://doi.org/10.1016/S0165-6147(02)00043-3
- Gradolatto A, Canivenc-Lavier MC, Basly JP, Siess MH, Teyssier C. Metabolism of apigenin by rat liver phase I and phase II enzymes and by isolated perfused rat liver. Drug Metabolism and Disposition. 2004 Jan; 32(1):58–65. PMid: 14709621. https://doi.org/10.1124/dmd.32.1.58
- Freeman F, Kodera Y. Garlic chemistry: Stability of S-(2propenyl)-2-propene-1-sulfinothioate (allicin) in blood, solvents and simulated physiological fluids. Journal of Agricultural and Food Chemistry. 1995 Sep; 43(9):2332–8. https://doi.org/10.1021/jf00057a004
- Jeong HJ, Koo HN, Na HJ, Kim MS, Hong SH, Eom JW, Kim KS, Shin TY, Kim HM. Inhibition of TNF-α and IL-6 production by aucubin through blockade of NF-κB activation in RBL-2H3 mast cells. Cytokine. 2002 Jun; 18(5):252–9. PMid: 12161100. https://doi.org/10.1006/cyto.2002.0894
- Kim SW, Choi SC, Choi EY, Kim KS, Oh JM, Lee HJ, Oh HM, Kim S, Oh BS, Kimm KC, Lee MH. Catalposide, a compound isolated from Catalpa ovata, attenuates induction of intestinal epithelial proinflammatory gene expression and reduces the severity of trinitrobenzene sulfonic acid-induced colitis in mice. Inflammatory Bowel Diseases. 2004 Sep; 10(5):564–72. PMid: 15472516. https://doi.org/10.1097/00054725200409000-00010
- Faggioni R, Fantuzzi G, Fuller J, Dinarello CA, Feingold KR, Grunfeld C. IL-1β mediates leptin induction during inflammation. Am J Physiol Regul Integr Comp Physiol. 1998; 274:R204–8. PMid: 9458919. https://doi.org/10.1152/ajpregu.1998.274.1.R204
- Sharma A, Sharma V, Kansal L. Amelioration of leadinduced hepatotoxicity by Allium sativum extracts in Swiss albino mice. Libyan Journal of Medicine. 2010 Jan; 5(1):4621. PMid: 28156294. https://doi.org/10.3402/ljm.v5i0.4621
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