Toxicology International (Formerly Indian Journal of Toxicology)

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Bio-Antioxidants Protect the Buffalo Bone Marrow Derived Mesenchymal Stem Cells against Oxidative Stress Induced During Freeze-Thaw Cycle


Affiliations
1 Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
2 Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
3 Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
     

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The present investigation was planned to evaluate the effect of curcumin (CUR) and resveratrol (RES) on oxidative stress indicators (LPO, O2- radical, ROS and TPC), antioxidant status (GSH, GPx, SOD, CAT and GST) and cytotoxicity markers (ALP, LDH, GGT and CK-MB) in mesenchymal stem cells (MSCs) derived from buffalo bone marrow during the freeze-thaw cycle of one month. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs were found to be positive for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4). Results revealed a significant reduction in (p≤0.05) in oxidative stress parameters and cytotoxicity markers, while, the levels of cellular antioxidants were found to be significantly increased (p≤0.05) in CUR/RES alone and in combination groups as compared to the control group. In conclusion, the addition of bioantioxidants in the cryopreservation medium has improved the post-thaw cell recovery through suppression of oxidative stress-induced during the freeze-thaw cycle.

Keywords

Antioxidants, Buffalo, Curcumin, Mesenchymal Stem Cells, Oxidative Stress, Resveratrol.
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  • Bio-Antioxidants Protect the Buffalo Bone Marrow Derived Mesenchymal Stem Cells against Oxidative Stress Induced During Freeze-Thaw Cycle

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Authors

M. Sharma.
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
Navjot Kaur.
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
D. Singh.
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
M. K. Lonare.
Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India
R. Udehiya.
Department of Veterinary Surgery and Radiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141001, Punjab., India

Abstract


The present investigation was planned to evaluate the effect of curcumin (CUR) and resveratrol (RES) on oxidative stress indicators (LPO, O2- radical, ROS and TPC), antioxidant status (GSH, GPx, SOD, CAT and GST) and cytotoxicity markers (ALP, LDH, GGT and CK-MB) in mesenchymal stem cells (MSCs) derived from buffalo bone marrow during the freeze-thaw cycle of one month. MSCs were isolated and cultured in high glucose DMEM supplemented with 15% FBS. MSCs were found to be positive for alkaline phosphatase (AP) activity and stem cell markers (CD73 and OCT4). Results revealed a significant reduction in (p≤0.05) in oxidative stress parameters and cytotoxicity markers, while, the levels of cellular antioxidants were found to be significantly increased (p≤0.05) in CUR/RES alone and in combination groups as compared to the control group. In conclusion, the addition of bioantioxidants in the cryopreservation medium has improved the post-thaw cell recovery through suppression of oxidative stress-induced during the freeze-thaw cycle.

Keywords


Antioxidants, Buffalo, Curcumin, Mesenchymal Stem Cells, Oxidative Stress, Resveratrol.

References





DOI: https://doi.org/10.18311/10.18311%2Fti%2F2021%2Fv28i1%2F24809