Toxicology International (Formerly Indian Journal of Toxicology)

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Effect of Ginkgo Biloba Extract on Histomorphometric Changes of Hippocampus Against Fluoride Toxicity In Wistar Rats


Affiliations
1 Department of Anatomy, Fathima Institute of Medical Sciences (FIMS), Dr. N.T.R. University of Health Sciences, Kadapa – 516003, Andhra Pradesh,, India
2 Department of Anatomy, Viswabharathi Medical College and General Hospital, Dr. N.T.R. University of Health Sciences, Kurnool – 518463, Andhra Pradesh, India
     

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Prolonged ingestion of fluoride leads to the pathogenesis known as fluorosis. Fluoride exposure leads to neuro degenerative changes such as reduction in the neuronal cell size and number. Exposure to fluoride showed neurodegenerative changes like shrunken neurons, increased in folding of the nuclear membrane, mitochondrial alterations, and dilated rough endoplasmic reticulum cisternae and clusters of vesicles near the Golgi bodies. Present study is to explore the histomorphometric changes of the hippocampus of fluoride induced brain and treated with Ginkgo biloba Extract (GBE). Thirty number (30) adult male Wistar rats were randomly separated into 5 Groups (n=6). Group1 (Control) supplemented with water, 2 to 5 Groups were supplemented with 100ppm of sodium fluoride for 30 days, while the Groups 3, 4, and 5 were Ginkgo biloba extract treated at 50mg/kg, 100mg/kg and 200mg/kg body weight for 15 days, after sodium fluoride treatment, results showed that, 200mg/kg GBE provided effective and complete protection against fluoride toxicity. From the present study concluded that 50, 100 and 200 mg/kg GBE was significantly attenuated the fluoride induced toxicity in a dose depending manner. Present histomorphometrical study showed delayed neuronal death in the CA1 and CA3 regions of hippocampus of fluoride group that was significantly attenuated by GBE (50,100 and 200 mg/kg) in a dose dependant manner.

Keywords

Fluoride, Ginkgo biloba, Hippocampus, Histomorphometry, Neuron.
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  • Effect of Ginkgo Biloba Extract on Histomorphometric Changes of Hippocampus Against Fluoride Toxicity In Wistar Rats

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Authors

Sugavasi Raju
Department of Anatomy, Fathima Institute of Medical Sciences (FIMS), Dr. N.T.R. University of Health Sciences, Kadapa – 516003, Andhra Pradesh,, India
Gudemalla Kanchanalatha
Department of Anatomy, Viswabharathi Medical College and General Hospital, Dr. N.T.R. University of Health Sciences, Kurnool – 518463, Andhra Pradesh, India

Abstract


Prolonged ingestion of fluoride leads to the pathogenesis known as fluorosis. Fluoride exposure leads to neuro degenerative changes such as reduction in the neuronal cell size and number. Exposure to fluoride showed neurodegenerative changes like shrunken neurons, increased in folding of the nuclear membrane, mitochondrial alterations, and dilated rough endoplasmic reticulum cisternae and clusters of vesicles near the Golgi bodies. Present study is to explore the histomorphometric changes of the hippocampus of fluoride induced brain and treated with Ginkgo biloba Extract (GBE). Thirty number (30) adult male Wistar rats were randomly separated into 5 Groups (n=6). Group1 (Control) supplemented with water, 2 to 5 Groups were supplemented with 100ppm of sodium fluoride for 30 days, while the Groups 3, 4, and 5 were Ginkgo biloba extract treated at 50mg/kg, 100mg/kg and 200mg/kg body weight for 15 days, after sodium fluoride treatment, results showed that, 200mg/kg GBE provided effective and complete protection against fluoride toxicity. From the present study concluded that 50, 100 and 200 mg/kg GBE was significantly attenuated the fluoride induced toxicity in a dose depending manner. Present histomorphometrical study showed delayed neuronal death in the CA1 and CA3 regions of hippocampus of fluoride group that was significantly attenuated by GBE (50,100 and 200 mg/kg) in a dose dependant manner.

Keywords


Fluoride, Ginkgo biloba, Hippocampus, Histomorphometry, Neuron.

References





DOI: https://doi.org/10.18311/ti%2F2022%2Fv29i1%2F28484