Toxicology International (Formerly Indian Journal of Toxicology)

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Amelioration of Streptozotocin Induced Cytotoxicity by Mimosa pudica Root Extract - A Proteomic Based In-Vitro Study


Affiliations
1 Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
2 Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam, India
3 Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India
     

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The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts.

Keywords

LC-MS, Mimosa pudica, Proteomics, RIN5F.
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  • Amelioration of Streptozotocin Induced Cytotoxicity by Mimosa pudica Root Extract - A Proteomic Based In-Vitro Study

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Authors

Pallavi S. Jamadagni
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Sharad D. Pawar
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Shrirang Jamadagni
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Shridhar Chougule
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Manish Wanjari
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Manish Gautam
Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam, India
Sudesh Gaidhani
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India
Arun Gurav
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
N. Shrikanth
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India

Abstract


The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts.

Keywords


LC-MS, Mimosa pudica, Proteomics, RIN5F.

References