Toxicology International (Formerly Indian Journal of Toxicology)

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Amelioration of Streptozotocin Induced Cytotoxicity by Mimosa pudica Root Extract - A Proteomic Based In-Vitro Study


Affiliations
1 Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
2 Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam, India
3 Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India
     

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The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts.

Keywords

LC-MS, Mimosa pudica, Proteomics, RIN5F.
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  • Ahuchaogu AA, Chukwu OJ, Echeme JO. Secondary metabolites from Mimosa Pudica: Isolation, purification and NMR characterization. IOSR Journal of Applied Chemistry. 2017; 10(3):15–20. https://doi:10.9790/5736-1003011520

  • Ahmad H, Sehgal S, Mishra A, Gupta R. Mimosa pudica L. (Laajvanti): An overview. Pharmacognosy Reviews. 2012; 6(12):115–24. PMid: 23055637 PMCid: PMC3459453, https://doi.org/10.4103/0973-7847.99945

  • Kokane DD, More RY, Kale MB, Nehete MN, Mehendale PC GC. Evaluation of wound healing activity of root of Mimosa pudica. J Ethnopharmacol. 2009; 124(2)8:311–5. PMid: 19397984. https://doi:10.1016/j.jep.2009.04.038

  • Sahu PR, Khalkho AS, Kumari S, Alam S. Studies on ethnomedicinal uses and formulation of herbal drugs from medicinal plants of Ranchi District - A survey. American Journal of Ethnomedicine [Internet]. 2015; 2(5):284–96. www.ajethno.comhttp://www.ajethno.com

  • Sunday RM, Obuotor EM, Kumar A. Antioxidant and antidiabetic properties of Mimosa pudica seeds in Streptozotocin-induced diabetic Wistar rats. Asian Journal of Biotechnology. 2019; 12(1):1–8. https://doi.org/10.3923/ajbkr.2020.1.8

  • Gupta R, Vairale MG, Deshmukh RR, Chaudhary PR, Wate SR. Ethnomedicinal uses of some plants used by Gond tribe of Bhandara district, Maharashtra. Indian Journal of Traditional Knowledge. 2010; 9(4):713–7. http://nopr.niscair.res.in/handle/123456789/10324

  • Joseph B, George J, Mohan J. Review article pharmacology and traditional uses of Mimosa pudica. International Journal of Pharmaceutical Sciences and Drug Research. 2013; 5(2):41–4. https://ijpsdr.com/index.php/ijpsdr/article/view/239.

  • Muhammad G, Hussain MA, Jantan I, Bukhari SNA. Mimosa pudica L., a high-value medicinal plant as a source of bioactives for pharmaceuticals. comprehensive reviews in Food Science and Food Safety. 2016; 15(2):303–15. PMid: 33371596. https://doi.org/10.1111/1541-4337.12184

  • Kaur P, Kumar N, Shivananda TN, Kaur G. Phytochemical screening and antimicrobial activity of the plant extracts of Mimosa pudica L. against selected microbes. Journal of Medicinal Plant Research. 2011; 5(22):5356–9. https://opensiuc.lib.siu.edu/ebl/vol2009/iss5/8

  • Chitra G, Athira KA, Anitha CT. Phytochemical screening and antibacterial activity of Mimosa pudica L. and Mimosa invisa L. against selected microbes. Nature Environment and Pollution Technology. 2012; 11(3):431-3. https://neptjournal.com/upload-images/NL-39-15-(15)B-1874

  • Anonymous. The Ayurvedic Formulary of India. In: The Ayurvedic Formulary of India. 2003. p. 1–110.

  • Ministry for Health and Family Welfare. The Ayurvedic Pharmacopoeia of India. Vol. 2, The Ayurvedic Pharmacopoeia of India. 2010. 171 p. http://www.ayurveda.hu/api/API-Vol-2

  • Banik G, Bawari M, Choudhury MD, Choudhury S, Sharma GD. Some anti-diabetic plants of Southern Assam. Assam University Journal of Science and Technology. 2010; 5(1):114–9. https://indiabiodiversity.org/biodiv/content/documents/document-ab21e2b6-05f4-47c0-9053-5b0abfcacb69/151

  • Adinortey MB, Agbeko R, Boison D, Ekloh W, Kuatsienu LE, Biney EE, Affum OO, Kwarteng J, Nyarko AK. Phytomedicines used for Diabetes Mellitus in Ghana: A systematic search and review of preclinical and clinical evidence. Evidence-based Complementary and Alternative Medicine. 2019; 2019. PMid:31118963 PMCid: PMC6500637. https://doi.org/10.1155/2019/6021209

  • Sutar NG, Sutar UN, Behera BC. Antidiabetic activity of the leaves of Mimosa pudica Linn. in albino rats. Journal of Herbal Medicine and Toxicology [Internet]. 2009; 3(1):123–6. https://www.researchgate.net/publication/268438284

  • Azwanida. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants. 2015; 04(03):3–8. https://doi.org/10.4172/2167-0412.1000196

  • Promega. Trypsin Gold, Trypsin Gold, Mass Spectrometry Grade, Instruction for Use of Product. 2015; 1–8. Available from: www.promega.com

  • Bateman A, Martin MJ, Orchard S, Magrane M, Agivetova R, Ahmad S, Alpi E, Bowler-Barnett EH, Britto R, Bursteinas B B-A-JH. UniProt: The universal protein knowledge base in 2021. Nucleic Acids Research. 2020; 49(8):D480–9. PMid: 33237286 PMCid: PMC7778908. https://doi.org/10.1093/nar/gkaa1100

  • Oliveros JC. VENNY. An interactive tool for comparing lists with Venn Diagrams. 2007. https://bioinfogp.cnb.csic.es/tools/venny/index.html

  • Wu J, Yan LJ. Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 2015; 8:181–8. PMid: 25897251 PMCid: PMC4396517. https://doi.org/10.2147/DMSO.S82272

  • Mardilovich K, Pankratz SL, Shaw LM. Expression and function of the insulin receptor substrate proteins in cancer. Cell Communication and Signaling. 2009; 7:1–15. PMid: 19534786 PMCid: PMC2709114. https://doi. org/10.1186/1478-811X-7-14

  • Sun XJ, Rothenberg P, Kahn CR, Backer JM, Araki E, Wilden PA, Cahill DA, Goldstein BJ, White MF. Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein. Nature. 1991; 352(6330):73–7. PMid: 1648180. https://doi:10.1038/352073a0

  • Pelicci G, Lanfrancone L, Grignani F, McGlade J, Cavallo F, Forni G, Nicoletti I, Grignani F, Pawson T, Pelicci PG. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction. Cell. 1992; 70(1):93–104. PMid: 1623525. https://doi.org/10.1016/0092-8674(92)90536-L

  • Zhou QL, Jiang ZY, Mabardy AS, Del Campo CM, Lambright DG, Holik J, Fogarty KE, Straubhaar J, Nicoloro S, Chawla A, Czech MP. A novel pleckstrin homology domain-containing protein enhances insulin-stimulated Akt phosphorylation and GLUT4 translocation in adipocytes. Journal of Biological Chemistry. 2010; 285(36):27581–9. PMid: 20587420 PMCid: PMC2934625. https://doi.org/10.1074/jbc.M110.146886

  • Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kučera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell. 2007; 129(2):359–70. PMid: 17448994. https://doi.org/10.1016/j.cell.2007.02.044


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  • Amelioration of Streptozotocin Induced Cytotoxicity by Mimosa pudica Root Extract - A Proteomic Based In-Vitro Study

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Authors

Pallavi S. Jamadagni
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Sharad D. Pawar
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Shrirang Jamadagni
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Shridhar Chougule
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Manish Wanjari
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
Manish Gautam
Assam Down Town University, Panikhaiti, Guwahati - 781026, Assam, India
Sudesh Gaidhani
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India
Arun Gurav
Regional Ayurveda Research Institute, Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, Gandhi Bhavan Road, Kothrud, Pune - 411038, Maharashtra, India
N. Shrikanth
Central Council for Research in Ayurvedic Sciences, Ministry of AYUSH, 61-65, Institutional Area, Opposite Janakpuri D Block, New Delhi - 110058, India

Abstract


The in-vitrocytotoxicity was studied in a pancreatic cell line (RIN5F) using streptozotocin-induced cellular damage. The Cytotoxic dose of aqueous and hydro-alcoholic extracts of Mimosa pudica root powder before and after streptozotocin treatment was studied and protein expression was explored. Both the extracts were subjected to metabolite analysis by LCMS. Mimosa pudica root powder extracts prevented Streptozotocin-induced cellular damage. Proteomics studies indicated both aqueous and hydro-alcoholic extracts of Mimosa pudica regulate proteins related to insulin secretion, glucose uptake, and insulin resistance with marked changes in proteins involved in insulin secretion.  The study first time reports cytotoxicity of Mimosa pudica in RIN5F cells and explores protein expression after Mimosa pudica treatment. It was also observed that the abundance of Mimosine is more in Hydro-alcoholic extracts as compared to aqueous extracts.

Keywords


LC-MS, Mimosa pudica, Proteomics, RIN5F.

References