Toxicology International (Formerly Indian Journal of Toxicology)

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Potential of Zingiber officinale Extract against Chlorpyrifos Toxicity in Caprine Testicular Cells


Affiliations
1 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
2 Department of Biochemistry, Sri Venkateshvara College, University of Delhi, Delhi – 110021, India
3 Department of Library and Information Science, Kurukshetra University, Kurukshetra – 136119, Haryana, India
     

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Chlorpyrifos, an organophosphate insecticide, is currently under studies due to its reproductive toxicity in non-targeted organisms. The present study on its efficacy as reproductive toxicant, revealed its effect on testicular cells of caprines. CPF was found to decrease cell viability in testicular cells, induced histo-architectural alterations like loss of tubular interactions and empty luminal spaces in testis; initiated DNA damage associated apoptosis as revealed by MTT, Histology and EB/AO fluorescence assay respectively, at both the doses (10 and 50 μM). CPF mediated toxicity induced oxidative stress in testicular cells as revealed by dose- and time-dependent increase in lipid peroxidation and decline in ferric reducing antioxidant power. However, supplementation of Zingiber officinale (ginger) extract reduced the CPF mediated decrease in cell viability, histological defects, incidence of apoptosis, and generated oxidative stress parameters; thus, proving its potential as a therapeutic agent against CPF, preventing testicular cells toxicity mediated reproductive disorders.

Keywords

Apoptosis, Chlorpyrifos, Testicular Cells, Zingiber officinale.
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  • Potential of Zingiber officinale Extract against Chlorpyrifos Toxicity in Caprine Testicular Cells

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Authors

Priyanka Saraf
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Harish Panchal
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Kameshwar Sharma
Department of Biochemistry, Sri Venkateshvara College, University of Delhi, Delhi – 110021, India
Sanjeev Sharma
Department of Library and Information Science, Kurukshetra University, Kurukshetra – 136119, Haryana, India
Jitender Kumar Bhardwaj
Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra – 136119, Haryana, India

Abstract


Chlorpyrifos, an organophosphate insecticide, is currently under studies due to its reproductive toxicity in non-targeted organisms. The present study on its efficacy as reproductive toxicant, revealed its effect on testicular cells of caprines. CPF was found to decrease cell viability in testicular cells, induced histo-architectural alterations like loss of tubular interactions and empty luminal spaces in testis; initiated DNA damage associated apoptosis as revealed by MTT, Histology and EB/AO fluorescence assay respectively, at both the doses (10 and 50 μM). CPF mediated toxicity induced oxidative stress in testicular cells as revealed by dose- and time-dependent increase in lipid peroxidation and decline in ferric reducing antioxidant power. However, supplementation of Zingiber officinale (ginger) extract reduced the CPF mediated decrease in cell viability, histological defects, incidence of apoptosis, and generated oxidative stress parameters; thus, proving its potential as a therapeutic agent against CPF, preventing testicular cells toxicity mediated reproductive disorders.

Keywords


Apoptosis, Chlorpyrifos, Testicular Cells, Zingiber officinale.

References