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As a thermal-mechanical-chemical pretreatment, steam explosion allows a synergism process of heating that disintegrates lignocellulosic structural components, as well as shearing forces that lead to moisture expansion. Essential oil refers to a complex mixture of compounds that are volatile and have strong odor. This research aimed to identify the influence of steam explosion as pretreatment process on the micro histological features of Citrus hystrix DC leaf and the yield of essential oil using hydrodistillation extraction. The fallowing process and material variables were used steam at pressure of 1-4 bar, and kaffir leaves-water ratio of 33%.  An application of steam explosion resulted in leaf tissue damaged and produced higher essential oil yield.  The highest yield of essential oil 1.386% (w/w) was obtained at the saturated pressure of 2 bar. Therefore, this study concludes steam explosion as a potential process to apply in essential oil extraction.


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