Asian Journal of Bio Science

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Isolation, Partial Purification, Product Formation and Characterization of β- Glucosidase from Roots of Hordeum vulgare L.


Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
     

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β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.


Keywords

Hordeum vulgare, β-Glucosidase, Purification, Characterization.
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  • Isolation, Partial Purification, Product Formation and Characterization of β- Glucosidase from Roots of Hordeum vulgare L.

Abstract Views: 243  |  PDF Views: 7

Authors

Suman Tiwari
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
O. P. Verma
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Shruti Shukla
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.


Keywords


Hordeum vulgare, β-Glucosidase, Purification, Characterization.