Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

In Vitro Micropropagation of Gerbera Using Auxillary Bud


Affiliations
1 Plant Tissue Culture Division, Jain Irrigation Systems Ltd., Jain Hills, Jalgaon, M.S., India
     

   Subscribe/Renew Journal


A protocol for rapid clonal micropropagation of gerbera was developed by auxillary bud.MS+BAP 2.0+NAA 0.5 mg/l, which was given early bud initiation (19.40 days), with higher initiation per cent (91.66%) along with longer shoot length (2.5 cm). MS+BAP 2.0+NAA 0.5+Ads 100 mg/ l produced higher number of shoots (7.33 shoots/explants). The micro shoots were ischolar_mained (96%) in just 8.56 days on MS+NAA 2.0+activated charcoal 750 mg/l. In vitro ischolar_mained plants were acclimatized on sand + soil + FYM+ leaf mould. Plants shows 82.43 per cent survival rate during acclimatization. The plants were established in the field after acclimatization.

Keywords

Gerbera, Auxillary Bud, Micropropagation
Subscription Login to verify subscription
User
Notifications
Font Size


  • Altaf, N., Khan, A.R., Ali, L. and Bhatti, I.A. (2009). Tissue culture of gerbera. Pakistan J. Bot., 41(1): 7-10.

  • Anonymous (2003). OmzettabelKamerplanten.Vakbl Bloemist., 21a: 136137.

  • Aswath, C.R. and Choudhary, M.L. (2002). Rapid plant regeneration fromGerbera jamesonii Bolus callus cultures. Acta Bot. Croatica, 61: 125-134.

  • Barbosa, M.H.P., Pinto, J.E.B.P., Pinto, C.A.B.P. and Innecco, R. (1994). In vitro propagation of Gerbera jamesonii Bolus ex Hook cv. Appelbloesem using young capitulums. Revista Ceres., 41: 386-395.

  • Bhatia, R., Singh, K.P. and Singh, M.C. (2008). Effect of growth regulators on regeneration from leaf derived callus and shoot proliferation in gerbera. Indian J. Hort., 65:312316.

  • Broek, Van Den L., Haydu, J.J., Hodges, A.W. and Neves, E.M. (2004). Production, marketing and distribution of cut flowers in the United

  • States and Brazil. Annual Report of Florida Agricultural Experiment Station, University of Florida. pp. 119.

  • Chebet, D.K., Okeno, J.A. and Mathenge, P. (2003). Biotechnological approaches to improve horticultural crop production. Acta Hort., 625: 473477.

  • Kanwar, J.K. and Kumar, S. (2008). In vitro propagation of Gerbera-a review. Hort. Sci. (Prague). 35:3544.

  • Kumar, S. and Kanwar, J.K. (2006). Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultured in vitro. Folia Hort., 18:57-64.

  • Meyer, H.J. and Staden, J.V. (1988). The In vitro culture of Gerbera aurantiaca. Plant Cell Tiss. Org. Cult., 14(1): 25-30.

  • Murashige, I. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant, 15: 473-487.

  • Naz, S., Naz, F., Tariq, A., Aslam F., Ali, A. and Athar, M. (2012). Effect of different explants on in vitro propagation of gerbera (Gerbera jamesonii). African J. Biotechnol., 11(37): 9048-9053.

  • Parthasarthy, V.A. and Nagaraju, V. (1996). Morphogenetic response of gerbera shoots to media and benzyl amino purine. Ann. Plant Physiol., 10: 3439.

  • Parthasarthy, V.A. and Nagaraju, V. (1999). In vitro propagation in Gerbera jamesonii Bolus. Indian J. Hort., 56: 8285.

  • Posada, M., Ballesteros, N., Obando, W. and Angarita, A. (1999). Micropropagation of gerbera from floral buds. Acta Hort., 482: 329-332.

  • Rajagopalan, C. (2000). Export potential of Indian floriculture and need of policy environment. Floric Today, 9:2933.

  • Reynoird, J.P., Chriqui, D., Noin, M., Brown, S. and Marie, D. (1993). Plant propagation from in vitro leaf culture of several gerbera species. Plant Cell Tiss. Org. Cult., 33: 203-210.

  • Schiva, T. (2000). Strategies for development of commercial floriculture in Asia and the Pacific. Report of the APO seminar, 2nd 6th May, 2000, NEW DELHI (INDIA). pp. 2738.

  • Schiva, T., Lercari, B. and Giusta, R. (1982). Micropropagation of gerbera: variable response to in vitro culture. AnnalidellIstituto-

  • Sperimentale-per-la-Floricoltura, 13: 56-61.

  • Sharma, G. and Srivastiva, R. (2005). Combinations and concentrations of growth regulators for somatic embryogenesis in gerbera. Ann. agric. Res., 26: 214217.

  • Thakur, P.S., Ghorade, R.B. and Rathod, T.H. (2004). Micropropagation studies in gerbera. Ann. Plant Physiol., 18: 133135.


Abstract Views: 413

PDF Views: 0




  • In Vitro Micropropagation of Gerbera Using Auxillary Bud

Abstract Views: 413  |  PDF Views: 0

Authors

A. R. Kadu
Plant Tissue Culture Division, Jain Irrigation Systems Ltd., Jain Hills, Jalgaon, M.S., India

Abstract


A protocol for rapid clonal micropropagation of gerbera was developed by auxillary bud.MS+BAP 2.0+NAA 0.5 mg/l, which was given early bud initiation (19.40 days), with higher initiation per cent (91.66%) along with longer shoot length (2.5 cm). MS+BAP 2.0+NAA 0.5+Ads 100 mg/ l produced higher number of shoots (7.33 shoots/explants). The micro shoots were ischolar_mained (96%) in just 8.56 days on MS+NAA 2.0+activated charcoal 750 mg/l. In vitro ischolar_mained plants were acclimatized on sand + soil + FYM+ leaf mould. Plants shows 82.43 per cent survival rate during acclimatization. The plants were established in the field after acclimatization.

Keywords


Gerbera, Auxillary Bud, Micropropagation

References